We narrowed to 13,360 results for: Mpl;

1. pAC8_MF-p10-Flag-Cter(VE5738)

   Plasmid

   #161798

   Purpose

   Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter Flag tag under the p10 promoter.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Insert

   C-terminal Flag tag
   

   Tags

   Flag

   Expression

   Insect

   Promoter

   p10

   Available Since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAC8_PH-10His-Nter-GWs-Lox (VE5588)

   Plasmid

   #161806

   Purpose

   Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter 10 His tag under the pH promoter.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Insert

   N-terminal 10His tag
   

   Tags

   10 His

   Expression

   Insect

   Promoter

   PH

   Available Since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC8_MF-p10-mCh PH-mCh (VE5625)

   Plasmid

   #139769

   Purpose

   Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an mCherry cDNA under each promoter.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Insert

   mCherry fluorescent protein
   

   Expression

   Insect

   Promoter

   PH or p10

   Available Since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIG-707pUC19-tNGFR-P2A-TTC37

   Plasmid

   #186122

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   TTC37 (SKIC3 Human)
   

   Use

   CRISPR

   Mutation

   WT with tNGFR sequence

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-MBP-Foldon-ATG9 (692-839)

   Plasmid

   #188087

   Purpose

   To make ATG9 C-terminal tail trimer for expression in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG9 (ATG9A Human)
   

   Tags

   MBP tag

   Expression

   Mammalian

   Mutation

   ATG9 (692-839)

   Promoter

   CMV

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBrain-GFP-TACC3KDP(S558A)-shTACC3

   Plasmid

   #59357

   Purpose

   Expresses GFP-tagged human TACC3 (RNAi-resistant) with S558A substitution and knocks down endogenous TACC3 via shRNA.

   Depositor

   Stephen Royle

   Article

   Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

   Insert

   Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
   

   Use

   RNAi

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Silent mutations in amino acids 30-33 to confer s…

   Promoter

   CMV

   Available Since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGAPTrap-mtagtdTom_IRESMuro

   Plasmid

   #82355

   Purpose

   Vector targeting the mTagtandem tomato gene to the GAPDH locus of human cells. It is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

   Depositor

   Ed Stanley

   Article

   Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

   Insert

   mtagTandemTomato-IRESMuro
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDF-casBCDE(g8)

   Plasmid

   #89727

   Purpose

   Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing 33-nt spacer (referred to as 'wt') matching a protospacer in the M13 phage genome.

   Depositor

   Konstantin Kuznedelov, Konstantin Severinov

   Article

   Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

   Insert

   CRISPR array
   

   Expression

   Bacterial

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEBTet-SNAP-Cep27

   Plasmid

   #136808

   Purpose

   Mammalian expression of the centrosomal protein Cep27 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-Cep27 (HAUS2 Human, Synthetic)
   

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Promoter

   CMV-TetO2

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKCAG-mCherry-OptoJNKi

    Plasmid

    #89738

    Purpose

    Expression of light-regulated JNK inhibitor, wild-type photosensor, inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    OptoJNKi
    

    Use

    Fluorescent protein

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMP108 Nanog-Venus-2a-mCherry

    Plasmid

    #159743

    Purpose

    Nanog targeting vector to insert fluorescent reporter of protein and gene expression levels from endogenous locus in mouse embryonic stem cells. Please see depositor comments for more detail.

    Depositor

    Sergi Regot

    Article

    Pokrass et al Dev Cell. 2020 Nov 9;55(3):328-340.e5. d

    Insert

    Nanog homeobox (Nanog Mouse)
    

    Use

    Mouse Targeting

    Tags

    Venus-2a-mCherry

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmCherry-NES-OptoJNKi

    Plasmid

    #89739

    Purpose

    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to cytoplasm, wild-type photosensor, inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    NLS-OptoJNKi
    

    Use

    Fluorescent protein

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKCAG-H2BmCherry-OptoJNKi

    Plasmid

    #89741

    Purpose

    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to chromatin with H2B, wild-type photosensor, inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    OptoJNKi
    

    Use

    Optogenetics

    Tags

    Histone2B and mCherry

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Epac-S-H96

    Plasmid

    #170342

    Purpose

    CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

    Insert

    CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available Since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST27-GST-ATXN1 FL(30Q)-S776D

    Plasmid

    #21755

    Depositor

    Huda Zoghbi

    Article

    Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 32 amino acids (30 Q…

    Promoter

    CMV

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pET28a-PTBP1-RRM12CCtoSS

    Plasmid

    #89155

    Purpose

    Recombinant expression of PTBP1-RRM12 C250S,C251S in E.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)
    

    Tags

    hexa His

    Expression

    Bacterial

    Mutation

    mutations: Cys 250 to Ser, Cys 251 to Ser

    Promoter

    T7

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pADSL-2XStrep

    Plasmid

    #125684

    Purpose

    expresses human ADSL protein with a C-terminal 2XStrep affinity tag

    Depositor

    Stephen Benkovic

    Article

    Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

    Insert

    ADSL (ADSL Human)
    

    Tags

    2X Strep-Tag II

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIRES:mTREK-1(K271Q)

    Plasmid

    #133273

    Purpose

    Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght K271Q mutant fused to a N-terminal HA tag

    Depositor

    Dan Minor

    Article

    Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

    Insert

    Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
    

    Tags

    HA

    Expression

    Mammalian and Yeast

    Mutation

    K271Q

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. p28BIOHTEV-LIC: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

    Plasmid

    #167848

    Purpose

    Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp10, nsp16
    

    Tags

    6xHis and AviTag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 22, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pGEX-6P-1 p37 SHPmut

    Plasmid

    #113503

    Purpose

    Bacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP box

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p37 (Ubxn2b Mouse)
    

    Tags

    GST-PreScission protease site

    Expression

    Bacterial

    Mutation

    F215A/E218A/QKL220-222AAA

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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