Addgene

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pRL-TK let7 I

Plasmid

#11332

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation I from figure 4A of paper.

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRLCon1-1746

Plasmid

#31446

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR (short) (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pRL-TK 4x mut G

Plasmid

#11320

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation G (see paper) on inner 2 binding sites.

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK CXCR4 2x

Plasmid

#11306

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

Insert

2 bulged bind sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pRL-TK let7 E

Plasmid

#11328

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation E from figure 4A of paper.

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK let7 F

Plasmid

#11329

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation F from figure 4A of paper.

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK let7 G

Plasmid

#11330

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation G from figure 4A of paper.

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRLCon850-1207

Plasmid

#31447

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragment (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pRL-TK let7 H

Plasmid

#11331

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation H from figure 4A of paper.

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRLCon1-449

Plasmid

#31452

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragment (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pRL-TK 4x mut I

Plasmid

#11322

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation I (see paper) on inner 2 binding sites.

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRLCon1-249

Plasmid

#31453

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragment (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pRLCon1-249_SNP

Plasmid

#31454

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragment (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

SNP rs11574744 in miR-34a binding site

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
OPRL1-DuET

Plasmid

#213360

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

OPRL1 (OPRL1 Human)

Use

Tags

1D4 and FLAG

Expression

Mammalian

Mutation

Promoter

CMV, T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Efg1-N-PrLD

Plasmid

#216397

Purpose

Expresses 6xHis-tagged N-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-N-PrLD

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

deleted amino acids 182-554

Promoter

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Efg1-C-PrLD

Plasmid

#216398

Purpose

Expresses 6xHis-tagged C-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-C-PrLD

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

Promoter

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
hPRL1_1-169

Plasmid

#209792

Purpose

Bacterial expression of human PRL-1 without farnesylation motif

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2020 Aug 14;295(33):11682-1

Insert

PRL-1 (PTP4A1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-169 only

Promoter

T7

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
hPRL1_1-169_C104D

Plasmid

#209793

Purpose

Bacterial expression of human PRL-1 C104D mutant without farnesylation motif

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2020 Aug 14;295(33):11682-1

Insert

PRL-1 (PTP4A1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-169 only; C101D

Promoter

T7

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
hPRL2_1-163

Plasmid

#209794

Purpose

Bacterial expression of human PRL-2 without farnesylation motif

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2020 Aug 14;295(33):11682-1

Insert

PRL-2 (PTP4A2 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-163 only

Promoter

T7

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
hPRL3_1-169_C104D

Plasmid

#209797

Purpose

Bacterial expression of human PRL-3 C104D mutant without farnesylation motif

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2020 Aug 14;295(33):11682-1

Insert

PRL-3 (PTP4A3 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-169 only; C101D

Promoter

T7

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge