-
Plasmid#44984DepositorInsertFUS ΔNLS (1-513aa) (FUS Human)
UseTagsGSTExpressionBacterialMutationcontains amino acids 1-513PromotertacAvailable sinceJune 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Su(fu) (CT#116)
Plasmid#13857DepositorInsertSu(fu) (SUFU Chicken)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX Su(fu) (CT#99)
Plasmid#13856DepositorInsertSu(fu) (SUFU Chicken)
UseTagsGSTExpressionBacterialMutationNco I fragment from full length; ~2kbp- ATG to st…PromoterAvailable sinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
TH1150-pOCC177-FUS(Nhe)-PLD_YtoF_RGG_RtoK
Plasmid#221889PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertFUS (FUS Human)
UseTags6xHis-MBP-PreScission and TEV-SNAP-tagExpressionInsectMutationIn PLD all Tyr (Y) changed to Phe (F), in RGG (aa…PromoterPH promoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
TH1148-pOCC177-FUS(Nhe)-PLD_YtoF
Plasmid#221887PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertFUS (FUS Human)
UseTags6xHis-MBP-PreScission and TEV-SNAP-tagExpressionInsectMutationIn PLD all Tyr (Y) changed to Phe (F)PromoterPH promoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
TH1149-pOCC177-FUS(Nhe)-RGG_RtoK
Plasmid#221888PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertFUS (FUS Human)
UseTags6xHis-MBP-PreScission and TEV-SNAP-tagExpressionInsectMutationIn RGG (aa 212-526) most of Arg (R) changed to Ly…PromoterPH promoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS Su(fu) (CT#117)
Plasmid#13858DepositorInsertSu(fu) (SUFU Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.2-FUS-1-526aa-V5
Plasmid#29609DepositorInsertFUS (FUS Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-sport2-mGCN5 FU, FLAG Tagged
Plasmid#23098DepositorInsertmouse Gcn5 mRNA (Kat2a Mouse)
UseTagsFLAGExpressionMammalianMutationmissing 50 bp upstream of ATG start site.PromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
RP1B FUS 1-163 QQ4xSS #1
Plasmid#127194PurposeProtein expression for affinity purificationDepositorInsertFUS 1-163 QQ4xSS #1 (FUS Human)
UseTagshexahistidineExpressionBacterialMutationQQ4xSS #1: Q27S, Q28S, Q93S, Q94S, Q132S, Q133S, …PromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.2-FUS-1-413aa-V5
Plasmid#29612DepositorInsertFUS-1-413aa (FUS Human)
UseTagsV5ExpressionMammalianMutationContains FUS aa# 1-413PromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.2-FUS-1-269aa-V5
Plasmid#29610DepositorInsertFUS-1-269aa (FUS Human)
UseTagsV5ExpressionMammalianMutationContains FUS aa# 1-269PromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.2-FUS-1-373aa-V5
Plasmid#29611DepositorInsertFUS-1-373aa (FUS Human)
UseTagsV5ExpressionMammalianMutationContains FUS aa# 1-373PromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
RP1B FUS 1-163 QQ4xSS #2
Plasmid#127195PurposeProtein expression for affinity purificationDepositorInsertFUS 1-163 QQ4xSS #2 (FUS Human)
UseTagshexahistidineExpressionBacterialMutationQQ4xSS #2:Q8S, Q9S, Q60S, Q62S, Q102, Q103, Q139S…PromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
TH0994-pOCC177-Fus-wt_(1-211)
Plasmid#221886PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertFUS (FUS Human)
UseTags6xHis-MBP-PreScission and TEV-SNAP-tagExpressionInsectMutationPLD domain (aa 1-211)PromoterPH promoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 myc his Su(fu) (CT#768)
Plasmid#13855DepositorInsertSu(fu) (SUFU Chicken)
UseTagshis and mycExpressionMammalianMutationfull ORFPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-Puro-P2A-NES/PKI-mNeonGreen2x-NLS/FUS
Plasmid#228400PurposeEncodes NES/PKI-mNeonGreen2x-NLS/FUS which is a tandem of mNeongreen with an N-terminal PKI NES and a C-terminal FUS NLS that shuttles between the nucleus and the cytoplasmDepositorInsertNES/PKI-mNeonGreen2x-NLS/FUS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-TetOn-FUS-CHOP-FusionRed-FRB-iLID-WPRE-SFFV-MBP-SspB-irFP-NLS-WPRE
Plasmid#223287PurposeA complete OptoMBP cassette for dissolution of FUS-CHOP condensates.DepositorInsertmyc-FUS-CHOP-FusionRed-FRB-iLID, MBP-SspB-irFP-NLS
UseSynthetic Biology; Piggybac transposase insertabl…TagsExpressionMammalianMutationPromoterAvailable sinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
MBP-FUS_FL_WT
Plasmid#98651PurposeExpresses MBP-tagged full length FUSDepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUS-WT-VC
Plasmid#175068Purposeexpresses FUS wildtype tagged to mVenus and mCherry in mammalian cellsDepositorInsertFUS glycine rich protein (FUS Human)
UseTagsHA and mVenus + mCherryExpressionMammalianMutationmVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…PromoterCMV PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-NFLAG-hFUS
Plasmid#50487PurposeProduces lentivirus expressing human FUS with FLAG tag at its N-terminus by co-transfection with psPAX2 and pMD2GDepositorInsertFUS (FUS Human)
UseLentiviralTagsFLAGExpressionMutationPromoterTREAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR4_FUS
Plasmid#26366DepositorInsertFUS (FUS Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceSept. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_FL_12E
Plasmid#98652PurposeExpresses MBP-tagged full length phosphomimetic FUSDepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutation12 S/T to EPromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_FL_6E
Plasmid#98655PurposeExpresses MBP-tagged full length phosphomimetic FUSDepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutation6 S/T to EPromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_LC_WT
Plasmid#98653PurposeExpresses MBP-tagged FUS AA 1-163DepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_LC_12E
Plasmid#98654PurposeExpresses MBP-tagged phosphomimetic FUS AA 1-163DepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutation12 S/T to EPromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR4_FLAG/HA_FUS_His6
Plasmid#26365DepositorInsertFUS (FUS Human)
UseEntry vectorTagsFLAG/HA and His6ExpressionMutationdelta 527 (stop codon)PromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
FUS_Halo_N_allele_400
Plasmid#161767PurposeDonor vector for endogenous tagging of human FUS at the N-terminus with halotagInserthalotag (FUS Human)
UseDonor vector for endogenous taggingTagsExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
CRY2olig-BFP
Plasmid#155355PurposeLentivector to express CRY2olig-BFP controlDepositorInsertcry2olig-BFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUS_Halo_C_allele_400
Plasmid#161768PurposeDonor vector for endogenous tagging of human FUS at the C-terminus with halotagInserthalotag (FUS Human)
UseDonor vector for endogenous taggingTagsExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSupAR-MbPylRS(DiZPK)
Plasmid#91705Purposeexpress Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interestDepositorInsertspylS
pylT
UseTagsExpressionBacterialMutationT810G, C813T, C820G, T821C, T937A, A1046T, G1257APromoterAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS U3-hTR-500
Plasmid#28170DepositorInsertU3-hTR-500 (TERC Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-GFP
Plasmid#86441Purposevisualization and quantifying the out-of-frame mutations from genome editingDepositorInsertmCherry-MCS-sfGFP fusion gene
UseColor visualization in bacteriaTagsmCherry-sfGFPExpressionMutationPromoterPlacAvailable sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLacZa-GFP
Plasmid#86440Purposevisualization and quantifying the out-of-frame mutations from genome editingDepositorInsertLacZalpha-MCS-sfGFP fusion gene
UseColor visualization in bacteriaTagsLacZa-sfGFPExpressionMutationPromoterPlacAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-hYTHDF3-N
Plasmid#155353PurposeExpress human YTHDF3(1-385) with a C-terminal SNAP-tag in mammalian cellsDepositorInsertYTHDF3(1-385)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-hYTHDF1-C2
Plasmid#155350PurposeExpress human YTHDF1(360-559) with a C-terminal SNAP-tag in mammalian cellsDepositorInsertYTHDF1 (360-559)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-hYTHDF1-C1
Plasmid#155349PurposeExpress human YTHDF1(264-559) with a C-terminal SNAP-tag in mammalian cellsDepositorInsertYTHDF1 (262-559)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-hYTHDF2-N
Plasmid#155352PurposeExpress human YTHDF2(1-381) with a C-terminal SNAP-tag in mammalian cellsDepositorInsertYTHDF2(1-381)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-hYTHDF1-N
Plasmid#155351PurposeExpress human YTHDF1(1-359) with a C-terminal SNAP-tag in mammalian cellsDepositorInsertYTHDF1 (1-359)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUS_LC_charge_RtoK
Plasmid#139127Purposeexpress FUS LC with hnRNPA2-like charge patterning where all R are changed to KDepositorInsertFUS (FUS Human)
UseTagsExpressionBacterialMutationAdd K and D to FUS LC so it has the same charge p…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUS_LC_charge
Plasmid#139126Purposeexpress FUS LC with an hnRNPA2 LC-like charge patterningDepositorInsertFUS (FUS Human)
UseTagsExpressionBacterialMutationAdd R, K, D to make FUS LC have similar charge pa…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-ApaLI-FLAG-T2A-mTagBFP2-Puro
Plasmid#211462PurposeLentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLIDepositorInsertApaLI
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterTREAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSET-mEosEM
Plasmid#132708Purposeapplication in Correlative light-electron microscopyDepositorInsertmEosEM
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-GFP1-10-IRES-Puro
Plasmid#211467PurposeLentivirus backbone expressing GFP1-10DepositorInsertGFP1-10
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHO4d-Cas9
Plasmid#67881PurposeCas9nlsHis6 Bacterial Expression constructDepositorInsertCas9
UseCRISPRTagsHis6 and T7 epitopeExpressionBacterialMutationPromoterT7Available sinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ApaLID211A-HA
Plasmid#211459PurposeExpress mitochondrial localized ApaLI with D211A mutationDepositorInsertApaLI-D211A
UseTagsHAExpressionMammalianMutationD211APromoterCMVAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ApaLI*-HA
Plasmid#211461PurposeExpress mitochondrial localized ApaLI with D211A, E225A and K227A mutationsDepositorInsertApaLI mutant
UseTagsExpressionMammalianMutationD211A, E225A, K227APromoterCMVAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ApaLIE225A, K227A-HA
Plasmid#211460PurposeExpress mitochondrial localized ApaLI with E225A and K227A mutationsDepositorInsertApaLI-E225A-K227A
UseTagsHAExpressionMammalianMutationE225A, K227APromoterCMVAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgSmg7
Plasmid#161809Purposeguide RNA for Smg7DepositorInsertsgRNA targeting mouse Smg7 (Smg7 Synthetic, Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only