We narrowed to 147 results for: Cas3

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pRSFDuet-3C-AcrIC8

Plasmid

#207410

Purpose

Protein purification vector to produce AcrIC8 in E. coli

Depositor

Yan Zhang

Article

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

T7-HRV3C-AcrIC8

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pPV-C1-crRNA-cloning-EF1a-BA

Plasmid

#204623

Purpose

piggyBac vector for crRNA cloning and expression. Blasticidin selectable.

Depositor

Akitsu Hotta

Article

Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pPV-EF1α-mRxxFP1(DMDex55_10)-iP-A

Plasmid

#204622

Purpose

Single-strand annealing (SSA) vector for monitoring crRNA(DMDex55)'s activity. DMD intron 55 region (downstream of exon 55). Puromycin selectable.

Depositor

Akitsu Hotta

Article

Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

Insert

mRFP1

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pPV-C1-crRNA(DMD#20_DMD#23)-EF1a-BA

Plasmid

#204620

Purpose

Expression vector of crRNA (DMD#20) targeting the dystrophin intron 44 just upstream of exon 45, and crRNA(DMD#23)targeting the dystrophin intron 55 just downstream of exon 55. Blasticidin selectable.

Depositor

Akitsu Hotta

Article

Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

Insert

crRNA#20 (targeting DMD intron44) and crRNA#23 (targeting DMD intron 55)

Use

CRISPR

Promoter

U6

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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D. vulgaris Cascade/I-C (Cas5c-Cas8c-Cas7)/pHMGWA

Plasmid

#81185

Purpose

Expresses Desulfovibrio vulgaris Cascade/I-C complex subunits in E. coli with TEV-cleavable N-terminal His-MBP tag on Cas5c

Depositor

Jennifer Doudna

Article

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Inserts

Cas5c
Cas8c
Cas7

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Promoter

T7

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pYZ1389

Plasmid

#215348

Purpose

Nla CRISPRi Delivery Vector

Depositor

Hank Seifert

Article

Geslewitz et al mBio. 2023 Dec 21:e0302523. doi: 10.1128

Insert

Nla CRISPRi Genes (Cas5, Cas8c, Cas7)

Use

CRISPR; Nla i delivery vector

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pEcCas6

Plasmid

#170091

Purpose

expresses E. coli type I-E Cas6

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cas6

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCDF-cascade

Plasmid

#247069

Purpose

L-arabinose inducible E.coli cascade operon inserted in pCDF vector

Depositor

Seokhee Kim

Article

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

E.coli Type I-E CRISPR cas

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMut3_pm32B

Plasmid

#247071

Purpose

L-arabinose inducible pmCDA-XTEN32-CasB(Cas11)-GSSG-UGI

Depositor

Seokhee Kim

Article

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

PmCDA-XTEN32-Cas11(CasB)-UGI

Use

CRISPR

Expression

Bacterial

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pMut20_tadDE32B

Plasmid

#247074

Purpose

L-arabinose inducible TadDE-XTEN32-CasB(Cas11)-GSSG-UGI

Depositor

Seokhee Kim

Article

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

TadDE-XTEN16-CasB-UGI

Use

CRISPR

Expression

Bacterial

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pMut19_tadDE16B

Plasmid

#247073

Purpose

L-arabinose inducible TadDE-XTEN16-CasB(Cas11)-GSSG-UGI

Depositor

Seokhee Kim

Article

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

TadDE-XTEN16-CasB-UGI

Use

CRISPR

Expression

Bacterial

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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D. vulgaris Cas7/pHMGWA

Plasmid

#81184

Purpose

Expresses Desulfovibrio vulgaris Cas7 in E. coli with TEV-cleavable N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Insert

Cas7

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Promoter

T7

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pJEC619

Plasmid

#182955

Purpose

SYNZIP18-Cascade.

Depositor

James Chappell

Article

Villegas Kcam et al ACS Synth Biol. 2022 Jan 25. doi: 10.102

Insert

SynZip18-Cascade

Tags

SynZip18

Expression

Bacterial

Mutation

SynZip18 fusion to CasA

Promoter

J23150

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pSth_Cas6

Plasmid

#178779

Purpose

Expression of S. thermophilus type I-E cas6

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

S. thermophilus type I-E cas6

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEcCas5

Plasmid

#170090

Purpose

expresses E. coli type I-E Cas5

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cas5

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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D. vulgaris Cas5c/pHMGWA

Plasmid

#81182

Purpose

Expresses Desulfovibrio vulgaris Cas5c in E. coli with TEV-cleavable N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Insert

Cas5c

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Promoter

T7

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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D. vulgaris Cas8c/pHMGWA

Plasmid

#81183

Purpose

Expresses Desulfovibrio vulgaris Cas8c in E. coli with TEV-cleavable N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Insert

Cas8c

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Promoter

T7

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pMut12_apo64C

Plasmid

#247072

Purpose

L-arabinose inducible rAPOBEC-XTEN64-CasC(Cas7)-GSSG-UGI

Depositor

Seokhee Kim

Article

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

rAPOBEC-XTEN64-CasC-UGI

Use

CRISPR

Expression

Bacterial

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEcCas7

Plasmid

#170092

Purpose

expresses E. coli type I-E Cas7

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cas7

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEcCas8

Plasmid

#170093

Purpose

expresses E. coli type I-E Cas8

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cas8

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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