We narrowed to 222 results for: EN2

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pCEP-Puro-TetOn-L1RP-ORF1_mNeonGreen2

Plasmid

#202566

Purpose

Expresses an endogenous-like L1RP LINE-1 element with a C-terminal mNeonGreen2 fluorophore on the ORF1 protein for fluorescence microscopy

Depositor

Liam Holt

Article

Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

Insert

LINE-1

Tags

mNeonGreen2 on ORF1p

Expression

Mammalian

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsStaufen2-T1_V

Plasmid

#147745

Purpose

Mammalian Expression of HsStaufen2-T1

Depositor

Elisa Izaurralde

Insert

HsStaufen2-T1 (STAU2 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pXD80EN2-Elen0501-(+)-Eddh-noAccs(ElA2)

Plasmid

#225310

Purpose

Expresses Eggerthella lenta A2 (+)-Eddh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eddh

Expression

Bacterial

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pXD70EN20-Pct5-GPA_25810-(+)-Eldh1(GpDSM19378)

Plasmid

#225306

Purpose

Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh1

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD70EN21-Pct5-GPA_25800-(+)-Eldh2(GpDSM19378)

Plasmid

#225307

Purpose

Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh2

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-EF1A-mNeonGreen2x-SREBP1-P2A-Puro

Plasmid

#228401

Purpose

Encodes a N-terminal truncated version of SREBP1 lacking part of the transcriptional activation domain and fused to a tandem of mNeongreen to visualize the import of SREBP1

Depositor

Dirk Daelemans

Article

Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

Insert

SREBP1 (SREBF1 Human)

Tags

mNeonGreen2x

Expression

Mammalian

Mutation

deleted N terminal activation domain (amino acids…

Promoter

EF1a

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0248 EF1a_mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin_T2A-mCherry_Gen2

Plasmid

#231208

Purpose

Single transcript cageIL-2 (mRens) + ER-renin (H2M) (Lenti)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

mRens-cageIL-2-c-myc_T2A_ERret-H2M-Renin_T2A-mCherry (IL2 Human)

Use

Lentiviral

Mutation

L80F, R81D, L85V, I86V, I92F

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0230 EF1a_Puro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry_Gen2

Plasmid

#231207

Purpose

Single transcript cageIL-10 (mRens) + ER-renin (H2M) (Lenti)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Puro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)

Use

Lentiviral

Mutation

L147I, R148H, I203V, L290V

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Puro-P2A-NES/PKI-mNeonGreen2x-NLS/FUS

Plasmid

#228400

Purpose

Encodes NES/PKI-mNeonGreen2x-NLS/FUS which is a tandem of mNeongreen with an N-terminal PKI NES and a C-terminal FUS NLS that shuttles between the nucleus and the cytoplasm

Depositor

Dirk Daelemans

Article

Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

Insert

NES/PKI-mNeonGreen2x-NLS/FUS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pEN244 - CTCF-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct

Plasmid

#92140

Purpose

Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using BLASTICIDIN selection. Auxin-inducible degron system.

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

AID[71-114]-eGFP

Use

Mouse Targeting

Expression

Mammalian

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBluescript Engrailed-2

Plasmid

#16950

Depositor

Richard Harland

Article

Hemmati-Brivanlou et al Development. 1991 Mar . 111(3):715-24.

Insert

Engrailed-2 (en2-b Frog)

Use

Xenopus probe

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only
TFORF1096

Plasmid

#143760

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

EN2 (EN2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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MAC_N_ETV6

Plasmid

#167755

Purpose

Expresses ETV6 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

ETV6 (EN2 Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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RET-WT

Plasmid

#125252

Purpose

Expresses human RET in mammalian cells

Depositor

Sissy Jhiang

Article

Xing et al Endocrinology. 1996 May;137(5):1512-9. d

Insert

RET (RET Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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RET-V5

Plasmid

#139627

Purpose

V5-His-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

RET (RET Human)

Tags

3xV5-6xHis

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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RET-SHC

Plasmid

#139617

Purpose

StrepIII-HA-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

RET (RET Human)

Tags

StrepIII-HA

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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RET_KD-V5

Plasmid

#139628

Purpose

V5-His-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

RET KD (RET Human)

Tags

3xV5-6xHis

Expression

Mammalian

Mutation

K758R

Promoter

CMV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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RET_Y1062F-SHC

Plasmid

#139620

Purpose

StrepIII-HA-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

RET_Y1062F (RET Human)

Tags

StrepIII-HA

Expression

Mammalian

Mutation

Y1062F

Promoter

CMV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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RET_Y981F-SHC

Plasmid

#139621

Purpose

StrepIII-HA-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

RET_Y981F (RET Human)

Tags

StrepIII-HA

Expression

Mammalian

Mutation

Y981F

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pET_mNG2(1-10)_32aalinker_mNG2(11)

Plasmid

#82611

Purpose

Bacterial expression vector for split mNeonGreen2 screening

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

mNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)

Expression

Bacterial

Mutation

split before mutations

Promoter

T7

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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