Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 154 results for: G2

Clear keywords
Showing: 61 - 80 of 154 results

  2. gRNA3_NIPBL_Cas9-hGem-for_N-terminal_tagging

    Plasmid
    #217662
    Purpose
    expresses Cas9-hGem and guideRNA for N terminal tagging of NIPBL
    Depositor
    Daniel Gerlich
    Publication
    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA3 for N terminal tagging of NIPBL (NIPBL Human)
    Use
    CRISPR
    Expression
    Bacterial and Mammalian
    Promoter
    U6
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    217662-plasmid-map-sequence-id-440854

    Enlarge

  4. AAVS1_Cas9-hGem_gRNA2

    Plasmid
    #217659
    Purpose
    expresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locus
    Depositor
    Daniel Gerlich
    Publication
    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA2 targeting the human AAVS1 safe harbour locus
    Use
    CRISPR
    Expression
    Bacterial and Mammalian
    Promoter
    U6
    Available since
    July 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    217659-plasmid-map-sequence-id-438226

    Enlarge

  5. pPtGE35

    Plasmid
    #107999
    Purpose
    Expresses TevCas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation
    Depositor
    David Edgell
    Publication
    Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    OriT
    40SRPS8 Promoter
    ShBle
    40SRPS8 Terminator
    Cen6-ArsH4-His3
    I-TevI nuclease and partial linker domain
    Use
    CRISPR and Synthetic Biology; Episomal vector for…
    Tags
    Cas9
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107999-plasmid-map-sequence-id-217151

    Enlarge

  8. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

    Plasmid
    #98975
    Purpose
    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.
    Depositor
    Jan Karlseder
    Publication
    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LMNA sgRNAs pair 4 and Cas9(D10A)
    Expression
    Mammalian
    Available since
    Nov. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    98975-plasmid-map-sequence-id-199218

    Enlarge

  9. pGMC00023 (aka pRC0434)

    Plasmid
    #195309
    Purpose
    Lentiviral expression vector derived from LEGO-G2 with G418/Neomycin selection
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195309-plasmid-map-sequence-id-385585

    Enlarge

  11. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

    Plasmid
    #98973
    Purpose
    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.
    Depositor
    Jan Karlseder
    Publication
    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LMNA sgRNAs pair 2 and Cas9(D10A)
    Expression
    Mammalian
    Available since
    Nov. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    98973-plasmid-map-sequence-id-199215

    Enlarge

  12. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3

    Plasmid
    #98974
    Purpose
    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.
    Depositor
    Jan Karlseder
    Publication
    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LMNA sgRNAs pair 3 and Cas9(D10A)
    Expression
    Mammalian
    Available since
    Nov. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    98974-plasmid-map-sequence-id-199217

    Enlarge

  13. pGMC00020 (aka pRC0431)

    Plasmid
    #195304
    Purpose
    Lentiviral expression vector derived from LEGO-G2 with blasticidin selection
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195304-plasmid-map-sequence-id-385590

    Enlarge

  14. pGMC00021 (aka pRC0432)

    Plasmid
    #195305
    Purpose
    Lentiviral expression vector derived from LEGO-G2 with puromycin selection
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    195305-plasmid-map-sequence-id-385584

    Enlarge

  17. pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1

    Plasmid
    #98976
    Purpose
    Cas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.
    Depositor
    Jan Karlseder
    Publication
    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LMNA sgRNAs pair 1 and Cas9(N863A)
    Expression
    Mammalian
    Available since
    Nov. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    98976-plasmid-map-sequence-id-199220

    Enlarge

  18. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

    Plasmid
    #98972
    Purpose
    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.
    Depositor
    Jan Karlseder
    Publication
    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LMNA sgRNAs pair 1 and Cas9(D10A)
    Expression
    Mammalian
    Available since
    Nov. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    98972-plasmid-map-sequence-id-199211

    Enlarge

  19. TFORF2151

    Plasmid
    #142920
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Publication
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXG1 (FOXG1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142920-plasmid-map-sequence-id-397278

    Enlarge

  20. pEG302 22aa SunTag NtDRMcd SUP

    Plasmid
    #115489
    Purpose
    CRISPR-Cas9 SunTag system to target NtDRMcd to the SUPERMAN locus with two guide RNAs
    Depositor
    Steven Jacobsen
    Publication
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    April 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    115489-plasmid-map-sequence-id-242029

    Enlarge

Showing: 61 - 80 of 154 results