We narrowed to 136 results for: G2

1. pcDNA3-HA+WT GADD45

   Plasmid

   #24929

   Depositor

   Xin Wang

   Article

   Yang et al J Biol Chem. 2000 Nov 24. 275(47):36892-

   Insert

   GADD45 (GADD45A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLL3.7m-YFP-SLBP(18-126)-IRES-H1-Skylan62A

   Plasmid

   #205760

   Purpose

   Single-color FUCCI4 sensor for S/G2 cell cycle transition and G2/M transition

   Depositor

   Edward Boyden

   Article

   Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

   Insert

   YFP-SLBP(18-126)-IRES-H1-Skylan62A
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   Available Since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. RepairTemplate_AAVS1-OsTIR1-F74G-SNAP

   Plasmid

   #217665

   Purpose

   Repair template to integrate OsTIR1-F74G-SNAP into the human AAVS1 safe harbour locus

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   OsTir1 F74G-SNAP-Blast-AAVS1
   

   Use

   Tags

   SNAP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS DRR mCherry Donor EF1a BFP

   Plasmid

   #98896

   Purpose

   Homologous recombination donor for pDRR. Expresses mCherry after repait by HR.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   Neomycin-T2A-mCherry-GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC CBA-SpCas9.EF1a-BFP.sgLMNA

   Plasmid

   #98971

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNA and spCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330_human CDK1

   Plasmid

   #118597

   Purpose

   One shot system to create CDK1as human cells. Encode Cas9 and guideRNA to inactivate human CDK1 gene.

   Depositor

   William Earnshaw

   Article

   Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

   Insert

   guide RNA against human CDK1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. BDS-2 3x WT (p53 binding site)

   Plasmid

   #16515

   Depositor

   Bert Vogelstein

   Article

   Hermeking et al Mol Cell. 1997 Dec . 1(1):3-11.

   Insert

   p53 binding site (SFN Human)
   

   Use

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pUHD Cdk2 WT HA

   Plasmid

   #27650

   Depositor

   Greg Enders

   Article

   Hu et al Mol Cell Biol. 2001 Apr . 21(8):2755-66.

   Insert

   Cdk2 (CDK2 Human)
   

   Use

   Tags

   HA (YDVPDYA)

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

9. BDS-2 3x MUT (p53 binding site)

   Plasmid

   #16516

   Depositor

   Bert Vogelstein

   Article

   Hermeking et al Mol Cell. 1997 Dec . 1(1):3-11.

   Insert

   p53 binding site (SFN Human)
   

   Use

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Mutant p53 binding sites

   Promoter

   Available Since

   March 28, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pUHD Cdk2 DN HA

    Plasmid

    #27651

    Depositor

    Greg Enders

    Article

    Hu et al Mol Cell Biol. 2001 Apr . 21(8):2755-66.

    Insert

    Cdk2 (CDK2 Human)
    

    Use

    Tags

    HA (YDVPDYA)

    Expression

    Mammalian

    Mutation

    D146N (Dominant Negative)

    Promoter

    Available Since

    Feb. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

11. KHBD00351

    Plasmid

    #34472

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG16778 (lov Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pLCN DSB Repair Reporter (DRR)

    Plasmid

    #98895

    Purpose

    DSB Repair Reporter (DRR). Expresses GFP after cut of two inverted ISce1 sites and repair by NHEJ.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    Neomycin-T2A-ISce1-ISce1-eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. RepairTemplate_NIPBL_mEGFP_mAID

    Plasmid

    #217664

    Purpose

    Repair template to endogenously tag NIPBL with mEGFP-mAID Tag

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    NIPBL_mEGFP_mAID (NIPBL Human)
    

    Use

    Tags

    mAID and meGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1_Cas9-hGem_gRNA2

    Plasmid

    #217659

    Purpose

    expresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locus

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    sgRNA2 targeting the human AAVS1 safe harbour locus
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. ATP1A1_G2_Dual_sgRNA

    Plasmid

    #173201

    Purpose

    Coselection for ABE or NHEJ in human cells. Vector for tandem expression of ATP1A1 G2 sgRNA with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G2 sgRNA + user-specified sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. mEmerald-PCNA-19

    Plasmid

    #54221

    Purpose

    Localization: Nucleus, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Lu et al J Cell Biol. 2010 Jun 14;189(6):967-80.

    Insert

    PCNA-19 (PCNA Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3

    Plasmid

    #98974

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 3 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

    Plasmid

    #98975

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 4 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5 FRT TO GFP hBub1 K821R

    Plasmid

    #59813

    Purpose

    Allows the integration of GFP hBub1 K821R in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Perera et al J Cell Sci. 2010 Mar 1;123(Pt 5):653-9.

    Insert

    Bub1 (BUB1 Human)
    

    Use

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    K821R

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5 FRT TO GFP hBub1 WT

    Plasmid

    #59812

    Purpose

    Allows the integration of GFP hBub1 in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Perera et al J Cell Sci. 2010 Mar 1;123(Pt 5):653-9.

    Insert

    Bub1 (BUB1 Human)
    

    Use

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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