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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,554 results for: ICL

Showing: 61 - 80 of 1554 results

  5. pcS-RDG-C1C2

    Plasmid
    #200163
    Purpose
    This plasmid encodes non-binder control monobody (RDG) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.
    Depositor
    Masako Harada
    Article
    Komuro et al Bioengineering (Basel). 2022 Jan 29;9(2)
    Inserts
    RDG-C1C2
    C1C2 domain of lactadherin
    Use
    Tags
    HA and HIS
    Expression
    Mammalian
    Mutation
    sequence adopted from doi: 10.1371/journal.pone.0…
    Promoter
    Available since
    Sept. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    200163-plasmid-map-sequence-id-409210

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  6. pEABR NA 3C FLAG SA

    Plasmid
    #234987
    Purpose
    For production of Extracellular Vesicles (EVs), with the transmembrane region of Neuraminidase protein fused to Streptavidin on the vesicles surface
    Depositor
    Daniel Leahy, Monica Gonzalez-Magaldi
    Article
    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.
    Insert
    EABR-NA
    Use
    Tags
    HRV 3C site, FLAG, Strep-Tactin (SA)
    Expression
    Mammalian
    Mutation
    In the Streptavidin coding sequence Glu44Val/Ser4…
    Promoter
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    234987-plasmid-map-sequence-id-468750

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  8. pLenti-PalmGRET

    Plasmid
    #158221
    Purpose
    BRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.
    Depositor
    Charles P. Lai
    Article
    Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014
    Insert
    PalmGFP-Nluc
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    158221-plasmid-map-sequence-id-309022

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  9. pHLsec-dasTMPRSS2

    Plasmid
    #207850
    Purpose
    Expresses directed activation strategy TMPRSS2 in mammalian cells, such as HEK293F- or CHO-cells
    Depositor
    Ingrid De Meester
    Article
    Verhulst et al J Extracell Vesicles. 2025 Mar;14(3):e70
    Insert
    transmembrane serine protease 2 (TMPRSS2 Human)
    Use
    Tags
    AviTag, 8xHIS tag
    Expression
    Mammalian
    Mutation
    deleted amino acid 1-108, S251D R252D Q253D S254D…
    Promoter
    CAG
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    207850-plasmid-map-sequence-id-419957

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  10. EP001: pLenti CMV Blast::eGFP-RC-I-1-H11

    Plasmid
    #209301
    Purpose
    Expression plasmid for RC-I-1-H11 (de novo virus-like particle designed by David Baker lab) with N-terminal eGFP
    Depositor
    Adam Cohen
    Insert
    RC-I-1-H11
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mutation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    209301-plasmid-map-sequence-id-414434

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  11. EP002: pLenti CMV Blast::HaloTag-RC-I-1-H11

    Plasmid
    #209302
    Purpose
    Expression plasmid for RC-I-1-H11 (de novo virus-like particle designed by David Baker lab) with N-terminal HaloTag
    Depositor
    Adam Cohen
    Insert
    RC-I-1-H11
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mutation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    209302-plasmid-map-sequence-id-414430

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  12. pOET5 rota-VLP

    Plasmid
    #218147
    Purpose
    Forms rotavirus-like particles in insect cells by expression of VP6 (GenBank ID: ACL93331.1).
    Depositor
    Minna Hankaniemi
    Insert
    Rotavirus VP6
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218147-plasmid-map-sequence-id-433323

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  14. pOET5 GII.4 Sydney noro-VLP

    Plasmid
    #218092
    Purpose
    Forms Sydney GII.4 Sydney norovirus-like particle in insect cells by expression of VP1 from strain GII.4/Sydney/NSW0514/2012/AU (GenBank ID: AFV08795).
    Depositor
    Minna Hankaniemi
    Insert
    noro-VLP
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218092-plasmid-map-sequence-id-433318

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  16. pPLP1H-mCf

    Plasmid
    #229984
    Purpose
    LIC vector for polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.
    Depositor
    Barry Lutz
    Article
    Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39
    Type
    Empty backbone
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229984-plasmid-map-sequence-id-458288

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  17. pPLP2H-mCf

    Plasmid
    #229985
    Purpose
    LIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.
    Depositor
    Barry Lutz
    Article
    Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39
    Type
    Empty backbone
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229985-plasmid-map-sequence-id-458287

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  18. pPLP3H-mCf

    Plasmid
    #229987
    Purpose
    LIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.
    Depositor
    Barry Lutz
    Article
    Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39
    Type
    Empty backbone
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229987-plasmid-map-sequence-id-458283

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  19. pOET5 CVB3-VLP

    Plasmid
    #218146
    Purpose
    Forms coxsackievirus 3-like particle in insect cells by expression of VP1–VP4 polyprotein (GeneID M33854.1) and 3CD protease (GeneID M33854.1).
    Depositor
    Minna Hankaniemi
    Inserts
    VP1–VP4
    3CD
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    CMV and Polyhedrin
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218146-plasmid-map-sequence-id-433321

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  20. MS2_SC2_S_Delta

    Plasmid
    #175950
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Delta SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Delta (Amino Acid 319-869)
    Promoter
    T7
    Available since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175950-plasmid-map-sequence-id-345521

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Showing: 61 - 80 of 1554 results