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  5. pRC49-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-2A-EGFP_NLS-WPRE

    Plasmid
    #123363
    Purpose
    Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP.
    Depositor
    Sidi Chen
    Article
    Chow et al Nat Methods. 2019 May;16(5):405-408. doi
    Insert
    EGFP-NLS
    Use
    CRISPR, Lentiviral, and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    123363-plasmid-map-sequence-id-240469

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  6. pU6-neo-TET

    Plasmid
    #51286
    Purpose
    expresses U6 RNA, using its own pol III enhancer sequence and tagged with the self splicing intron neo cassette
    Depositor
    Astrid Roy-Engel
    Article
    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:
    Insert
    U6 (RNU6-1 Human)
    Use
    Tags
    neoTET cassette with a tetrahymena self-splicing …
    Expression
    Mammalian
    Mutation
    Promoter
    U6 upstream enhancer sequence
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    51286-plasmid-map-sequence-id-78368

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  10. pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #190033
    Purpose
    vector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promoters
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized hfCas13X
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Y672A,Y676A
    Promoter
    CBh, SV40, hU6
    Available since
    Sept. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    190033-plasmid-map-sequence-id-371640

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  11. pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #190034
    Purpose
    vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized hfCas13d
    Use
    Tags
    Expression
    Mammalian
    Mutation
    A134V,A140V,A141V,A143V
    Promoter
    CBh, SV40, hU6
    Available since
    Sept. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    190034-plasmid-map-sequence-id-371641

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  12. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid
    #175570
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    XbaI site added upstream of U6 promotor. G to C …
    Promoter
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    175570-plasmid-map-sequence-id-344992

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  13. pDG330

    Plasmid
    #100898
    Purpose
    SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100898-plasmid-map-sequence-id-195326

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  14. PtPuc3_diaCas9_sgRNA

    Plasmid
    #109219
    Purpose
    Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum
    Depositor
    Per Winge
    Article
    Sharma et al Sci Rep. 2018 Sep 26;8(1):14401. doi: 10
    Inserts
    LHCF2 promoter
    diaCas9
    LHCF1 terminator
    U6 promoter
    sgRNA
    U6 3' region
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    LHCF1 terminator, LHCF2 promoter, U6 3' regi…
    Available since
    June 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    109219-plasmid-map-sequence-id-260727

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  15. pDG335

    Plasmid
    #100899
    Purpose
    SpCas9n (D10A Nickase mutant) with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100899-plasmid-map-sequence-id-195324

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  17. pCRUSH

    Plasmid
    #54480
    Purpose
    Mouse genomic ROSA26 targeting vector, expresses EGFP, cre/lox conditional U6 promoter pgk-puro
    Depositor
    Laurie Jackson-Grusby
    Article
    Brown et al Genesis. 2014 Jan;52(1):39-48. doi: 10.1
    Type
    Empty backbone
    Use
    Cre/Lox, Mouse Targeting, and RNAi
    Tags
    SA-EGFP-polyA, U6 promoter, loxP, and pgk-puro
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 18, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. pCFD3.1-w-dU6:3gRNA

    Plasmid
    #123366
    Purpose
    gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).
    Depositor
    Simon Bullock
    Insert
    U6:3-gRNA
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    April 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    123366-plasmid-map-sequence-id-242548

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  19. pJR255

    Plasmid
    #78549
    Purpose
    For expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. CMV driven mCherry visible marker.
    Depositor
    John Rossi
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6, CMV
    Available since
    Jan. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    78549-plasmid-map-sequence-id-191088

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  20. pJR288

    Plasmid
    #78550
    Purpose
    For expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. Ef1a driven mCherry visible marker.
    Depositor
    John Rossi
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6, EF1a
    Available since
    Jan. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    78550-plasmid-map-sequence-id-191096

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Showing: 781 - 800 of 4687 results