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pAP693-3

Plasmid

#99492

Purpose

nirFP::3xFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

nirFP::3xFlag

Expression

Bacterial

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pJH1922

Plasmid

#132361

Purpose

Prgef-1 INS-1::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-1::GFP

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

INS-1 (ins-1 Nematode)

Tags

GFP

Expression

Worm

Promoter

Prgef-1

Available Since

Oct. 28, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCG34

Plasmid

#26940

Depositor

Geraldine Seydoux

Article

Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

Insert

cgh-1 (cgh-1 Nematode)

Tags

GFP

Expression

Worm

Available Since

June 22, 2011

Availability

Academic Institutions and Nonprofits only

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pJRK253

Plasmid

#173750

Purpose

CeLINC plasmid

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Phsp-16.48::CRY2(olig)::VHH(GFP)::unc-54 3' UTR

Expression

Worm

Promoter

Phsp-16.48

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pNH013

Plasmid

#42147

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1

Use

Synthetic Biology

Available Since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pOX-SK4

Plasmid

#16116

Depositor

Larry Salkoff

Article

Wei et al Neuropharmacology. 1996 . 35(7):805-29.

Insert

small conductance Ca2+-activated K+ channel SK4 (B0399.1 Nematode)

Use

Xenopus oocyte expression

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pMS4 (Empty insertion vector with SEC)

Plasmid

#154837

Purpose

Vector that can be modified to add gene(s) of interest to C. elegans ChrII:8420157 site via CRISPR with SEC selection

Depositor

Patrick Phillips

Article

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

SEC

Use

CRISPR and Cre/Lox

Expression

Worm

Mutation

sqt-1(e1350)

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pGEM-GluCl_cryst

Plasmid

#31487

Depositor

Eric Gouaux

Article

Hibbs et al Nature. 2011 Jun 2. 474(7349):54-60.

Insert

C. elegans glutamate-gated chloride channel alpha, crystallized construct

Use

For rna synthesis for expression in x. laevis ooc…

Tags

8x-His

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pSR06

Plasmid

#69152

Purpose

read-outloxN mCherry to GFP switch for integration on ttTi5605, Mos Chr II

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
eGFP

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0 and codon-optimzed index…

Promoter

rps-27

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pJL2

Plasmid

#184130

Purpose

For rescuing null mrp-1 mutation and labelling mrp-1 protein. Translational fusion construct. pPD95.75_Pmrp-1::mrp-1(isoform C cDNA)::gfp.

Depositor

Alejandro Aballay

Article

Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

Insert

MRP-1 (mrp-1 Nematode)

Tags

GFP

Expression

Worm

Promoter

Pmrp-1

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pJL6

Plasmid

#184134

Purpose

For labelling (RFP) the apical region of the intestine. Translational fusion construct. coel::RFP_Pvha-6::vha-6::dsred.

Depositor

Alejandro Aballay

Article

Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

Insert

CELE_VW02B12L.1 (vha-6 Nematode)

Tags

RFP

Expression

Worm

Promoter

Pvha-6 (894bp)

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pJL1

Plasmid

#184129

Purpose

For rescuing null mrp-1 mutation. pPD95.77_Pmrp-1::mrp-1(isoform C cDNA)::SL2::gfp. SL2 prevents fusion of MRP-1 and GFP.

Depositor

Alejandro Aballay

Article

Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

Insert

MRP-1 (mrp-1 Nematode)

Tags

GFP

Expression

Worm

Promoter

Pmrp-1 (3kb)

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pJL3

Plasmid

#184131

Purpose

For rescuing null mrp-1 mutation and labelling mrp-1 protein. Intestinal specific Pges-1 promoter drives MRP-1 expression. Translational fusion construct. pPD95.75_Pges-1::mrp-1(isoform C cDNA)::gfp.

Depositor

Alejandro Aballay

Article

Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 1

Insert

MRP-1 (mrp-1 Nematode)

Tags

GFP

Expression

Worm

Promoter

Pges-1 (3.3kb)

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pJW1759

Plasmid

#154315

Purpose

NT Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::TEV::AID*::3xFLAG for NT slot

Use

CRISPR

Expression

Worm

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJH2827

Plasmid

#179542

Purpose

Pnmr-1::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of miniSOG-SL2 RFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Tags

wCherry

Expression

Worm

Promoter

Pnmr-1

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCM3.11

Plasmid

#19605

Depositor

Geraldine Seydoux

Insert

pgl-1 ORF+3' in pDONR201 (pgl-1 Nematode)

Expression

Worm

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pGC48

Plasmid

#19679

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R2-R1)-L4440

Use

Gateway Cloning and RNAi

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pClneoEGFPC RSF-1

Plasmid

#66862

Purpose

Express GFP-fused C.elegans RSF-1 in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

Insert

RSF-1 (rsf-1 Nematode)

Tags

GFP

Expression

Mammalian

Mutation

S18G, S229T, I403T and F563S mutations in RSF-1 c…

Promoter

CMV

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pGC235

Plasmid

#19683

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Plim-7

Use

Gateway Cloning

Available Since

March 3, 2009

Availability

Academic Institutions and Nonprofits only

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pCFJ150-cul4

Plasmid

#80353

Purpose

Pmex-5:GFP::H2B::PEST::cul-4 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Article

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::cul-4 3'UTR

Expression

Worm

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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