1,862 results

1. pCFJ2214 - Intron GG1 - 900 bp

   Plasmid

   #159880

   Purpose

   Golden Gate compatible intron with PATCs for reduced germline silencing

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   Intron GG1 - 900 bp
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCZGY3195

   Plasmid

   #172695

   Purpose

   Punc-25-gfp1-10 Expresses split GFP parts 1-10 specifically in C. elegans GABAergic motor neurons (only shows active fluorescence when binds to GFP11)

   Depositor

   Yishi Jin

   Article

   Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

   Insert

   Punc-25
   

   Use

   Tags

   GFP 1-10

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAS2-his-24::mNeptune

   Plasmid

   #124332

   Purpose

   Expressing Nuclear Localized mNeptune

   Depositor

   Andrew Leifer

   Insert

   mNeptune (his-24 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV myc DAF-12

   Plasmid

   #32566

   Depositor

   Al Fisher

   Article

   Hochbaum et al PLoS Genet. 2011 Jul;7(7):e1002179. Epub

   Insert

   DAF-12 (daf-12 Nematode)
   

   Use

   Tags

   myc epitope

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDD316

   Plasmid

   #137020

   Purpose

   mTurq2-GLO^SEC^2xHA vector with ccdB markers for cloning homology arms

   Depositor

   Daniel Dickinson, Bob Goldstein

   Insert

   mTurq2-GLO^SEC^2xHA + ccdB
   

   Use

   CRISPR and Cre/Lox

   Tags

   2xHA and C. elegans GLO-optimized mTurq2

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBN281

   Plasmid

   #86726

   Purpose

   Tissue-specific FLP expression plasmid for C. elegans

   Depositor

   Peter Askjaer

   Article

   Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

   Insert

   Plin-31::FLP D5
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSR35

   Plasmid

   #69256

   Purpose

   phsp16.41::CRE for integration on ttTi14024, Chr X

   Depositor

   Sander van den Heuvel

   Article

   Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

   Insert

   Cre recombinase
   

   Use

   Cre/Lox; Mossci

   Tags

   Expression

   Worm

   Mutation

   codon-optimzed index 1.0

   Promoter

   hsp16.41

   Available since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGC158

   Plasmid

   #19620

   Depositor

   Jane Hubbard

   Article

   Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

   Insert

   Plag-2::FLP::let-858(3’)
   

   Use

   Flp/frt

   Tags

   Expression

   Worm

   Mutation

   Promoter

   lag-2 promoter

   Available since

   March 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBN258

   Plasmid

   #86721

   Purpose

   Tissue-specific FLP expression plasmid for C. elegans

   Depositor

   Peter Askjaer

   Article

   Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

   Insert

   Punc-119::FLP D5
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFB-GluCl_cryst

    Plasmid

    #31488

    Depositor

    Eric Gouaux

    Article

    Hibbs et al Nature. 2011 Jun 2. 474(7349):54-60.

    Insert

    C. elegans glutamate-gated chloride channel alpha, crystallized construct
    

    Use

    Tags

    8x-His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pGC240

    Plasmid

    #19625

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Plim-7

    Use

    Flp/frt

    Tags

    4xNLS

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNM3810

    Plasmid

    #154119

    Purpose

    mec-4 promoter driving QF in an RCME vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    mec-4p::QF::act-4 '3
    

    Use

    Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOD2049-csnControl

    Plasmid

    #100007

    Purpose

    MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2048-csnDEG. Expression is controlled by Posm-6 (ciliated sensory neuron specific).

    Depositor

    Karen Oegema

    Article

    Wang et al Development. 2017 Jun 15. pii: dev.15009

    Inserts

    zif-1 (zif-1 Nematode)
    unc-119
    

    Use

    Targeting vector for mos1 transposon mediated sin…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMLS297

    Plasmid

    #73737

    Purpose

    SapTrap STOP codon + let-858 UTR N-terminal connector donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    STOP codon + let-858 3'-UTR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pISlin41w(s) (lin-41 microRNA wt reporter)

    Plasmid

    #11906

    Depositor

    David Bartel

    Article

    Lewis et al Cell. 2003 Dec 26. 115(7):787-98.

    Insert

    lin-41 miRNA target sequence (lin-41 Nematode)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJW4.21

    Plasmid

    #62875

    Purpose

    Bacterial expression of MBP-MEG-1

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    MBP-MEG-1
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCM5.34

    Plasmid

    #21390

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    fbf-2 3'utr in pDONRP2RP3
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS2-his-24::tagBFP

    Plasmid

    #124320

    Purpose

    Expressing Nuclear Localized tagBFP

    Depositor

    Andrew Leifer

    Insert

    tagBFP (his-24 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMDJ16 - pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]

    Plasmid

    #159802

    Purpose

    Expression construct with ubiquitous expression to test fluorescence level on microscope

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCFJ914

    Plasmid

    #44489

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1 [Peft-3 GFP H2B tbb-2 UTR, NeoR]
    

    Use

    Gateway expression clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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