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BiDi-[mEGFP]mCherry2

Plasmid

#182875

Purpose

Bi-directional expression vector of monomers

Depositor

Salvatore Chiantia

Article

Petazzi et al J Virol. 2020 Nov 25. pii: JVI.01238-20.

Insert

mCherry2 and mEGFP

Expression

Mammalian

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph

Plasmid

#218366

Purpose

Empty backbone for Tn7-site insertion

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pDSAR

Plasmid

#62292

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, DsRed RFP marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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BiDi-[mCherry2]mEGFP

Plasmid

#182874

Purpose

Bi-directional expression vector of monomers

Depositor

Salvatore Chiantia

Article

Petazzi et al J Virol. 2020 Nov 25. pii: JVI.01238-20.

Insert

mEGFP, mCherry2

Expression

Mammalian

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-shCIAP2-B

Plasmid

#44132

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

CIAP2

Use

Lentiviral and RNAi

Available Since

March 29, 2013

Availability

Academic Institutions and Nonprofits only
pET14b_tev_PDZ2

Plasmid

#59734

Purpose

expresses PDZ2 domain of human ZO-1 in bacteria

Depositor

Alan Fanning

Article

Fanning et al J Biol Chem. 2007 Dec 28;282(52):37710-6

Insert

zonula occludens-1 PDZ2 (TJP1 Human)

Tags

HIS and TEV

Expression

Bacterial

Available Since

Sept. 11, 2014

Availability

Academic Institutions and Nonprofits only
pDSAP

Plasmid

#62293

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, Puromycin resistance marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pDSAY

Plasmid

#62291

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, YFP marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO-shcIAP1A

Plasmid

#44129

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

cIAP1

Use

Lentiviral and RNAi

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only
T7-PseB-His6

Plasmid

#89723

Purpose

Expresses PseB from C. jejuni with an N-terminal T7 tag and a C-terminal His6 tag

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

PseB

Tags

His6

Expression

Bacterial

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSM038

Plasmid

#128293

Purpose

asymmetric plasmid partitioning E. coli - helper plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

PphlF - sfyfp-P2A-motA

Expression

Bacterial

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSPB866

Plasmid

#128304

Purpose

asymmetric plasmid partitioning E. coli - regulatory plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Para - GCN4zip.MSE-cfp-parB

Expression

Bacterial

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_P3-ehaA_LacI

Plasmid

#186315

Purpose

Plasmid for inducible E coli expression of the P3 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

P3-ehaA

Expression

Bacterial

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_AP4-ehaA_LacI

Plasmid

#186316

Purpose

Plasmid for inducible E coli expression of the AP4 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

AP4-ehaA

Expression

Bacterial

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pEGF/HisB-cGK1

Plasmid

#106451

Purpose

expression of murine cyclic GMP-dependent kinase

Depositor

Georg Weber

Article

He et al Biochem Biophys Res Commun. 2004 Aug 13;

Insert

cyclic GMP-dependent kinase (Prkg1 Mouse)

Tags

6xHis

Expression

Mammalian

Mutation

WT

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDL14

Plasmid

#17837

Depositor

Jay Keasling

Article

Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

Insert

gpdA promoter, lacZ, trpC

Use

Synthetic Biology; A. nidulans

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
pGEM cWnt14 (CT#692)

Plasmid

#13947

Depositor

Cliff Tabin

Article

Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

Insert

Wnt-14 (WNT9A Chicken)

Expression

Bacterial

Mutation

Note that this insert is in the reverse direction…

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pmRuby2-IRES-Clover

Plasmid

#171059

Purpose

Expresses mRuby2 and mClover2 in the cytoplasm as negative control for FRET.

Depositor

Ke Xu

Article

Chen et al Light Sci Appl. 2021 May 8;10(1):97. doi

Insert

IRES

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pSARSF534-3

Plasmid

#45618

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

NpuDnaEC35-GFP_C

Tags

H6

Expression

Bacterial

Mutation

SYG->CYG in chromophore

Promoter

T7

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pDL11

Plasmid

#17832

Depositor

Jay Keasling

Article

Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

Insert

alcA promoter, lacZ, argB,

Use

Synthetic Biology; Aspergillus nidulans expression

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
pDSAG

Plasmid

#62289

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, GFP marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCC48

Plasmid

#128285

Purpose

asymmetric plasmid partitioning E. coli - target plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Piq - mRFP, BBa_J23100 - phlF, parS

Expression

Bacterial

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pDSARN

Plasmid

#62295

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, DsRed-NLS marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDSAT

Plasmid

#62290

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, mTurquoise CFP marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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XP1 Xwnt-1

Plasmid

#16990

Depositor

Randall Moon

Article

Wolda et al Dev Biol. 1993 Jan. 155(1):46-57.

Insert

Xwnt-1 (wnt1.L Frog)

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pDL13

Plasmid

#17836

Depositor

Jay Keasling

Article

Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

Insert

alcA promoter, lacZ, trpC

Use

Synthetic Biology; A. nidulans

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
p3E-nlsGFP pA

Plasmid

#80814

Purpose

nlsGFP with pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

nlsGFPpA 3'E

Expression

Mammalian

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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SMX DEST vector

Plasmid

#62399

Purpose

Empty Backbone

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-c2G

Plasmid

#75290

Purpose

Expresses proteins in the cytoplasm as fusions to maltose-binding protein

Depositor

Paul Riggs

Article

Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

Type

Empty backbone

Tags

maltose-binding protein

Expression

Bacterial

Promoter

tac

Available Since

July 20, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pattB-RfB2

Plasmid

#62296

Purpose

docking site transgenesis plasmid for Gateway Cloning and insect transgenesis

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Use

Unspecified

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDSAYN

Plasmid

#62294

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, YFP-NLS marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSABAD219

Plasmid

#45596

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

NpuDnaEC102 intein

Tags

GB1-H6

Expression

Bacterial

Promoter

pBAD

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pSADuet502

Plasmid

#46373

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

NpuDnaB intein

Tags

GB1 and H6-GB1

Expression

Bacterial

Promoter

T7

Available Since

Sept. 16, 2013

Availability

Academic Institutions and Nonprofits only

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Rv2002 M3/pET-28b(+)N-tag

Plasmid

#13057

Depositor

SeWon Suh

Article

Yang et al Proc Natl Acad Sci U S A. 2003 Jan 21. 1

Insert

3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase

Tags

His

Expression

Bacterial

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
p0AmpBC

Plasmid

#237275

Purpose

Empty vector for L0 TypeIIS

Depositor

Eric Young

Article

Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

Type

Empty backbone

Use

Synthetic Biology

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
p1ChlEF

Plasmid

#237277

Purpose

Empty vector for L1 TypeIIS

Depositor

Eric Young

Article

Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

Type

Empty backbone

Use

Synthetic Biology

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
p1ChlFG

Plasmid

#237278

Purpose

Empty vector for L1 TypeIIS

Depositor

Eric Young

Article

Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

Type

Empty backbone

Use

Synthetic Biology

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pHREP-KD-1

Plasmid

#240416

Purpose

Sleeping Beauty vector for inducible expression of an shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.

Depositor

Lienhard Schmitz

Article

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Type

Empty backbone

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pTE5608

Plasmid

#250514

Purpose

Cell-free expression of SecA motor protein for translocation through SecYEG

Depositor

Tobias Erb

Article

Meier et al bioRxiv 2025.12.09.688994

Insert

T7-secA

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pTE5639

Plasmid

#250519

Purpose

Superactive inserter mutant of pSecYEG; cell-free expression of functional SecYEG

Depositor

Tobias Erb

Article

Meier et al bioRxiv 2025.12.09.688994

Insert

T7-secY(T166L)EG

Expression

Bacterial

Mutation

T166L

Promoter

T7

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
p2KanEG

Plasmid

#237279

Purpose

Empty vector for L2 TypeIIS

Depositor

Eric Young

Article

Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL051

Plasmid

#242609

Purpose

Parental vector for building the dual-sgRNA CRISPRi library. modified mU6-sgRNA library vector

Depositor

Brittany Adamson

Article

Simpson et al bioRxiv [Preprint]. 2023 Aug 20:2023.08.

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified mouse U6

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL052

Plasmid

#242610

Purpose

Parental vector for building the dual-sgRNA CRISPRi library. modified hU6-sgRNA library vector

Depositor

Brittany Adamson

Article

Simpson et al bioRxiv [Preprint]. 2023 Aug 20:2023.08.

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified human U6

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
p0AmpCD

Plasmid

#237276

Purpose

Empty vector for L0 TypeIIS

Depositor

Eric Young

Article

Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p0AmpAB

Plasmid

#237274

Purpose

Empty vector for L0 TypeIIS

Depositor

Eric Young

Article

Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLB025

Plasmid

#234634

Purpose

pET-28a(+) based plasmid for expression of H16_A1358 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_A1358

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJB1465

Plasmid

#218625

Purpose

Express engineered TFs Adr1c(S230A), Pip2c, and Oaf1c

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Adr1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJB1401

Plasmid

#218615

Purpose

Express Pex13, Pex14, Pex17, Pex2, Pex10, and Pex12

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex13

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKS1469

Plasmid

#218588

Purpose

Express peroxisomal MvaSp and MvaEp to convert AcCoA to mevalonate

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

MvaS

Tags

ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC3-C-GFP

Plasmid

#222601

Purpose

Expression of MAC3-tagged GFP (C-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge

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