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pBEL2296

Plasmid

#164601

Purpose

Complementation plasmid for MlaD (A20F)

Depositor

Damian Ekiert, Gira Bhabha

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Use

Tags

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE)-MlaD(Ala20Phe)-MlaC-MlaB

Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2294

Plasmid

#164599

Purpose

Complementation plasmid for MlaD (F13A)

Depositor

Damian Ekiert, Gira Bhabha

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Use

Tags

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE)-MlaD(Phe13Ala)-MlaC-MlaB

Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2290

Plasmid

#164596

Purpose

Complementation plasmid for MlaD (G11F)

Depositor

Damian Ekiert, Gira Bhabha

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Use

Tags

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE)-MlaD(Gly11Phe)-MlaC-MlaB

Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL1647

Plasmid

#155148

Purpose

Expresses MlaF 1-246, tagless

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-246)

Use

Tags

Expression

Mutation

Promoter

araBAD

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1840

Plasmid

#155150

Purpose

Expresses MlaF-6xHis

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-6xHis

Use

Tags

Expression

Mutation

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1266

Plasmid

#155142

Purpose

Expresses MlaF, tagless, araBAD promoter

Depositor

Damian Ekiert, Gira Bhabha

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF

Use

Tags

Expression

Mutation

Promoter

araBAD

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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JB42

Plasmid

#128924

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rac1QL-BirA*-HA-MAO (RAC1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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JB54

Plasmid

#128925

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rac1TN-BirA*-HA-MAO (RAC1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pTK643

Plasmid

#114703

Purpose

Express NuMA(214-705)-RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA(214-705)-RFP-Nano

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TetO-Snu114-fSNAPc

Plasmid

#106989

Purpose

open reading frame of Snu114 inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

Snu114

Use

Tags

fSNAP

Expression

Mutation

Promoter

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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rpaA_NS1

Plasmid

#101829

Purpose

drives expression of rpaA from the NS1 site in the S. elongatus PCC7942 genome

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

RpaA with its native promoter

Use

Cyanobacteria cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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P0050::rpaA

Plasmid

#101830

Purpose

drives expression of rpaA from an rpaA-independent promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

RpaA with p0050 promoter (+ gentamicin resistance casette)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Ptrc::galP_NS1

Plasmid

#101843

Purpose

drives expression of galP transporter from NS1 locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

Ptrc promoter driving GalP transporter expression

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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C064

Plasmid

#174353

Purpose

Plasmid for expressing frog PABPC1 (C-His) in E.coli

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

PABPC1 (pabpc1.S Frog)

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pAG/MNase

Plasmid

#123461

Purpose

Expresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.

Depositor

Steven Henikoff

Article

Meers et al Elife. 2019 Jun 24;8. pii: 46314. doi: 1

Insert

Protein A-Protein G-Micrococcal Nuclease

Use

Tags

6xHIS and HA

Expression

Bacterial

Mutation

Promoter

Lac

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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GAL4BD-VP16/pDON207

Plasmid

#201230

Purpose

GAL4-VP16 transcription factor

Depositor

Michael Ayliffe

Article

Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

Insert

GAL4BD-VP16

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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pME Integrin beta 1b

Plasmid

#105976

Purpose

entry clone for gateway cloning, Full length Integrin beta 1b without stop

Depositor

Caren Norden

Article

Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

Insert

Integrinbeta1b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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NES-mCherry-P4Mx1

Plasmid

#108143

Purpose

PI4P biosensor

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

X.leavis map2k1.L(32-44):mCherry:L. penomophila SidM(546-647)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFsynW TeTx-LC

Plasmid

#197284

Purpose

Encodes for viral expression of the light chain of tetanus toxin.

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

Tetanus toxin light chain

Use

Lentiviral

Tags

Expression

Mutation

N/A

Promoter

hSyn (human synapsin I promoter)

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-tdMCP-msfGFP(V206K)-GBI

Plasmid

#205163

Purpose

express tdMCP-msfGFP(V206K)-GBI

Depositor

Weirui Ma

Article

Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

Insert

tdMCP-msfGFP(V206K)-GBI

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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