Addgene

We narrowed to 10,960 results for: Vars;

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pEM7070

Plasmid

#214008

Purpose

PrpsM driven expression of ssrA-tagged mNeonGreen (ASV)

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mNeonGreen-ASV

Use

Tags

Expression

Bacterial

Mutation

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM7074

Plasmid

#214012

Purpose

PrpsM driven expression of ssrA-tagged mCerulean (ASV)

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mCerulean-ASV

Use

Tags

Expression

Bacterial

Mutation

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM7068

Plasmid

#214006

Purpose

PrpsM driven expression of ssrA-tagged eGFP (ASV)

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

eGFP-ASV

Use

Tags

Expression

Bacterial

Mutation

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDBD-M

Plasmid

#50572

Purpose

Double Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50571

Depositor

Alexander Varshavsky

Article

Turner et al Science. 2000 Sep 22;289(5487):2117-20.

Insert

DHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin

Use

Tags

Expression

Yeast

Mutation

Promoter

Gal1

Available since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only
pRB1081

Plasmid

#71479

Purpose

Expresses gRNA for dpy-10 conversion using NGAG PAM and VQR Cas9 variant

Depositor

Andrew Fire

Article

Bell et al Genetics. 2015 Dec 17. pii: genetics.115

Insert

dpy-10 gRNA

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pLL3.7_hsa-miR-30c

Plasmid

#25797

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

hsa-miR-30c

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only
pRBUBR1

Plasmid

#20198

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

Insert

UBR1 (UBR1 Budding Yeast)

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pR208IIM1

Plasmid

#45352

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

Insert

UBR1 mutant

Use

Tags

Expression

Yeast

Mutation

P406S, DE317NK

Promoter

ADH

Available since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
pR208IM1

Plasmid

#45351

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

Insert

UBR1 mutant

Use

Tags

Expression

Yeast

Mutation

D176E

Promoter

ADH

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pGL3-PMT-CD41

Plasmid

#25801

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

CD41 promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
pCB116

Plasmid

#53365

Purpose

To express Cup9p‐FLAG from the PGAL1 promoter

Depositor

Alexander Varshavsky

Article

Byrd et al EMBO J. 1998 Jan 2. 17(1):269-77.

Insert

Cup9 (CUP9 Budding Yeast)

Use

Tags

Flag

Expression

Yeast

Mutation

Promoter

GAL1

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pCB210

Plasmid

#31722

Purpose

To express Cup9p‐FLAG from the PCUP9 promoter

Depositor

Alexander Varshavsky

Article

Byrd et al EMBO J. 1998 Jan 2. 17(1):269-77.

Insert

CUP9 (CUP9 Budding Yeast)

Use

Tags

Cup9 promoter and FLAG

Expression

Yeast

Mutation

Promoter

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pKDC.154

Plasmid

#222228

Purpose

Acceptor plasmid for human retron-Eco1 variants.

Depositor

Seth Shipman

Article

Crawford et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEM7065

Plasmid

#214003

Purpose

PrpsM driven expression of ssrA-tagged mCerulean (LVA)

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mCerulean-LVA

Use

Tags

Expression

Bacterial

Mutation

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM7063

Plasmid

#214001

Purpose

PrpsM driven expression of ssrA-tagged eGFP (LVA)

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

eGFP-LVA

Use

Tags

Expression

Bacterial

Mutation

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM8731

Plasmid

#213991

Purpose

PrpsM driven expression of mNeonGreen - sequence codon-optimized for Salmonella

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mNeonGreen

Use

Tags

Expression

Bacterial

Mutation

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM7064

Plasmid

#214002

Purpose

PrpsM driven expression of ssrA-tagged mNeonGreen (LVA)

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mNeonGreen-LVA

Use

Tags

Expression

Bacterial

Mutation

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM9177

Plasmid

#213994

Purpose

PrpsM driven expression of SSAGSAAGS-mGFPmut2

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

SSAGSAAGS-mGFPmut2

Use

Tags

SSAGSAAGS

Expression

Bacterial

Mutation

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM7071

Plasmid

#214009

Purpose

PrpsM driven expression of ssrA-tagged mScarlet-I (ASV)

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mScarlet-I-ASV

Use

Tags

Expression

Bacterial

Mutation

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pNB687

Plasmid

#60119

Purpose

Yellow beetle luciferase

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

YeLuc

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge