We narrowed to 159,258 results for: addgene

1. SPIN2B (SPIN2BA-c011)

   Plasmid

   #98238

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   SPIN2BA (SPIN2B Human)
   

   Use

   Tags

   TEV-His6-Flag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TMPRSS2 g1 + Cas9

   Plasmid

   #153015

   Purpose

   A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

   Depositor

   Jason Sheltzer

   Insert

   Cas9 + TMPRSS2 gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP2

   Expression

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 4P7A

   Plasmid

   #61954

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   MLH1:JMC01L-F09:C205690 (MLH1 Human)
   

   Use

   Tags

   6xHis and MHHHHHHSSGRENLYFQG

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available since

   March 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. SMYD3

   Plasmid

   #32048

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SMYD3 (SMYD3 Human)
   

   Use

   Tags

   His (with TEV cleavage)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pTFAM.1.0-gDNA

   Plasmid

   #132443

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   TFAM (TFAM Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PRDM11

   Plasmid

   #32858

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PRDM11 (PRDM11 Human)
   

   Use

   Tags

   His (with TEV cleavage)

   Expression

   Bacterial

   Mutation

   amino acids 79-314

   Promoter

   Available since

   Jan. 6, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pTF271 (pFA6a-TEV-6xGly-12xHis-HphMX)

   Plasmid

   #44081

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   12xHis tag, 6xGly linker, ADH1 transcription term…

   Expression

   Mutation

   Promoter

   Available since

   April 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p-dCas9-p300-S1396R-S1397R-Hygro

   Plasmid

   #104419

   Purpose

   transient expression of dCas9-dp300 fusion protein

   Depositor

   Stephan Beck

   Insert

   dp300
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   S1396R-S1397R

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. integrin beta6 Y774F pBabe puro

   Plasmid

   #13599

   Depositor

   Dean Sheppard

   Insert

   integrin beta 6 Y774F (ITGB6 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Y774F

   Promoter

   Available since

   Jan. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

10. integrin alpha9 EGFP pBabe puro

    Plasmid

    #13605

    Depositor

    Dean Sheppard

    Insert

    integrin alpha 9 (ITGA9 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pPyCAG-Nanog(K140A)-IP

    Plasmid

    #62998

    Purpose

    Expresses Mouse Nanog K140A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).

    Depositor

    Shinya Yamanaka

    Insert

    Mouse Nanog K140A Mutant (Nanog Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Lysine 140 (141 AA in Mouse Protein) to A…

    Promoter

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pZC11 (pFA6a-TEV-6xGly-V5-KanMX)

    Plasmid

    #44097

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    6xGly linker, ADH1 transcription terminator, KanM…

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. XLone-BSD NFIL3 P2A eGFP NLS

    Plasmid

    #140030

    Purpose

    Tunable and temporal expression control of NFIL3 and nuclear eGFP

    Depositor

    Xiaoping Bao

    Insert

    NFIL3 (NFIL3 Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTF267 (pFA6a-TEV-6xGly-12xHis-KanMX)

    Plasmid

    #44093

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    12xHis tag, 6xGly linker, ADH1 transcription term…

    Expression

    Mutation

    Promoter

    Available since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CBS

    Plasmid

    #73238

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CBS (CBS Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV H2afy-V5

    Plasmid

    #175122

    Purpose

    Lentiviral expression of mouse Macroh2a1-V5

    Depositor

    Larry Gerace

    Insert

    Macroh2a1 (Macroh2a1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTF275 (pFA6a-TEV-6xGly-2xStrep-His3MX)

    Plasmid

    #44070

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    2xStrep tag, 6xGly linker, ADH1 transcription ter…

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. cTRE-PtenWT

    Plasmid

    #135663

    Purpose

    Introduce Dox-inducible (TRE promoter) wildtype Pten cDNA into (ES) cells by recombination-mediated cassette exchange

    Depositor

    Florian Karreth

    Insert

    Pten (Pten Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTF280 (pFA6a-PSc-6xGly-2xStrep-HphMX)

    Plasmid

    #44076

    Depositor

    Tim Formosa

    Type

    Empty backbone

    Use

    Pcr-based yeast c-terminal tagging

    Tags

    2xStrep tag, 6xGly linker, ADH1 transcription ter…

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGRHL2.1.0-gDNA

    Plasmid

    #175863

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    GRHL2 (GRHL2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge