We narrowed to 1,583 results for: pbluescript

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fabp10a:GCaMP6s; cryaa:mCherry

Plasmid

#230060

Purpose

Expression of genetically encoded calcium indicator in zebrafish hepatocytes.

Depositor

Sumeet Pal Singh

Article

Pozo-Morales et al Hepatology. 2023 Mar 1;77(3):789-801. do

Insert

GCaMP6s

Use

Zebrafish expression

Promoter

fabp10a

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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HEK4-53bp-tag-insertion-pegRNA

Plasmid

#227673

Purpose

PegRNA for 53 bp insertion

Depositor

Scot Wolfe

Article

Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

Insert

SpCas9 sgRNA scaffold (EPHA3 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pJBM3

Plasmid

#225290

Purpose

E. coli-Campylobacter shuttle vector; KanR

Depositor

Robert Moore

Article

McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

Insert

Replication elements from pCC388 for pJBM3

Expression

Bacterial

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pMK486 (Hygro-P2A-2mAID)

Plasmid

#214398

Purpose

2mAID for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

Hygro-P2A-2mAID

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMK446 (BSD-P2A-BromoTag-mClover)

Plasmid

#214376

Purpose

BromoTag-mClover for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BSD-P2A-BromoTag-mClover

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMK447 (BSD-P2A-BromoTag-mCherry)

Plasmid

#214377

Purpose

BromoTag-mCherry for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BSD-P2A-BromoTag-mCherry

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMK451 (BSD-P2A-dTAG)

Plasmid

#214381

Purpose

dTAG for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BSD-P2A-dTAG

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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p1153

Plasmid

#222930

Purpose

Shuttle vector carrying a Candida auris centromere (CEN 4) with Nourseothricin selectable marker

Depositor

Brian Wickes

Article

Determann B et al J Fungi (Basel). 2024 Jul 11;10(7):477.

Insert

CEN 4

Expression

Bacterial

Available Since

July 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBstSK+IRES-V5-IPpoI

Plasmid

#206321

Purpose

A plasmid that facilitates production of mRNA encoding V5 epitope tagged I-PpoI

Depositor

Brian McStay

Article

van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

Insert

IRES element-V5 epitope tag- IPpoI open reading frame

Use

Mrna production

Tags

V5-IPpoI fusion protein

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro EGFP nLAP +0

Plasmid

#194305

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(EGFP)

Use

Bacterial cloning vector

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMK438 (Stag-3FLAG-P2A-Hygro)

Plasmid

#168233

Purpose

C-terminal tagging with Stag-3FLAG

Depositor

Masato Kanemaki

Article

Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

Insert

Stag-3FLAG-P2A-Hygro

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-URA-GAL1-fSNAP-3xHA

Plasmid

#188935

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-fSNAP-3xHA

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-HALO-Hygromycin

Plasmid

#188929

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-HALO-Hygromycin

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fCLIP-URA

Plasmid

#188922

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-URA

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fCLIP-NAT

Plasmid

#188921

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-NAT

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pJJS009

Plasmid

#188332

Purpose

PCR template for repair templates to tag a gene with a mKate2 and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

mIAA7::mKate2::Linker

Expression

Bacterial

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJJS003

Plasmid

#188326

Purpose

PCR template for repair templates to tag a gene with a mCherry and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

mIAA7::mCherry::Linker

Expression

Bacterial

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-tRNA-Ser

Plasmid

#174318

Purpose

For in vitro transcription of mouse tRNAs

Depositor

Bruce Beutler

Article

Yue et al Science. 2021 May 14;372(6543). pii: 372

Insert

tRNA-ser

Expression

Bacterial

Promoter

T7

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDS1904

Plasmid

#175596

Purpose

Integrative vector for Candida albicans with dominant SAT1 marker

Depositor

Dominique Sanglard

Article

Dorsaz et al Front Microbiol. 2017 Aug 3;8:1478. doi:

Type

Empty backbone

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pKSB-610 U6-sgRNA2 GGAA AGAG

Plasmid

#173672

Purpose

To clone and express a gRNA in Anopheles

Depositor

Eric Marois

Article

Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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