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pCMV-MfPylRS (human-opti)

Plasmid

#164081

Purpose

To express a Methanosarcina flavescens derived PylRS in mammalian cells for genetic code expansion

Depositor

Tao Liu

Article

Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

Insert

MfPylRS(human-opti)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-MtPylRS (human-opti)

Plasmid

#164080

Purpose

To express a Methanosarcina thermophila derived PylRS in mammalian cells for genetic code expansion

Depositor

Tao Liu

Article

Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

Insert

MtPylRS-478(human-opti)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_aa 1-420 POLI

Plasmid

#201582

Purpose

Expresses the catalytic domain (aa 1-420) of human DNA polymerase iota with a HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

DNA polymerase iota (POLI Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Expresses amino acids 1-420 of DNA polymerase iota

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_aa 1-449 POLH

Plasmid

#201583

Purpose

Expresses the catalytic domain (aa 1-449) of human DNA polymerase eta with a HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

POLH (POLH Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Expresses amino acids 1-449 of DNA polymerase eta

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_aa 1-542 POLK

Plasmid

#201585

Purpose

Expresses the N-terminal clasp domain and catalytic domain (aa 1-542) of human DNA polymerase kappa with a HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

Polymerase kappa (POLK Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Expresses amino acids 1-542 of DNA polymerase kap…

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-Neo-ISG15-LRAA

Plasmid

#80405

Purpose

overexpression of nonconjugative ISG15

Depositor

Isidoro Martinez

Article

Gonzalez-Sanz et al J Virol. 2016 Jan 13;90(7):3428-38. doi:

Insert

ISG15 (ISG15 Human)

Use

Tags

Expression

Mammalian

Mutation

nonconjugative ISG15, LRAA

Promoter

CMV

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-FLAG-mRuby2

Plasmid

#175294

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with mRuby2

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-Frankenbody-FLAG-mEGFP

Plasmid

#175293

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with mEGFP

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-PARP1-3xFlag-7xKQ

Plasmid

#111576

Purpose

Expresses full length PARP1 with K to Q mutations in automodification domain in mammalian cells

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

PARP1 (PARP1 Human)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

lysine to glutamine mutation at the following ami…

Promoter

CMV

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-FLAG-Halo

Plasmid

#175296

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with HaloTag

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam APC

Plasmid

#16507

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

APC (APC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-FLAG-SNAP

Plasmid

#175295

Purpose

Track mature FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with SNAP-tag

Use

Tags

SNAP-tag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-SARS-CoV-2 Nsp3TMC111A

Plasmid

#204207

Purpose

Mammalian expression of SARS-CoV-2 Nsp3

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )

Use

Tags

FLAGx1

Expression

Mammalian

Mutation

Cys to Ala in active site of PLpro

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2-Sox9 T236A

Plasmid

#111455

Purpose

Transient expression of Flag-tagged human Sox9 with T236A mutation

Depositor

Sharon Pine

Article

Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

Insert

Sox9 (SOX9 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

T236A mutation

Promoter

CMV

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcmv3-STING-C88S-FLAG

Plasmid

#198187

Purpose

Mammalian expression of FLAG-tagged mouse STING1 C88S

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

TMEM173 (Sting1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2-Sox9 T240A

Plasmid

#111457

Purpose

Transient expression of Flag-tagged human Sox9 with T240A mutation

Depositor

Sharon Pine

Article

Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

Insert

Sox9 (SOX9 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

T240A

Promoter

CMV

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-XPACK-DNAJC5 (L115R)

Plasmid

#205717

Purpose

Mammalian expression of XPACK-DNAJC5 (L115R)

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

XPACK (GCINSKRKD)

Expression

Mammalian

Mutation

L115R

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-DNAJC5 (L115R)-HaloTag

Plasmid

#205711

Purpose

Mammalian expression of DNAJC5 (L115R)-HaloTag

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

L115R

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-mTagBFP2-FRB-Rab7

Plasmid

#214273

Purpose

Mammalian expression of mTagBFP2-FRB-Rab7 (late endosome marker)

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

mTagBFP2-FRB-Rab7A (RAB7A Canis lupus familiaris (Dog))

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcmv3-STING-C1471S-FLAG

Plasmid

#198188

Purpose

Mammalian expression of FLAG-tagged mouse STING1 C1471S

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

TMEM173 (Sting1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

The cysteine in STING at position 147 mutates to …

Promoter

CMV

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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