Addgene

We narrowed to 1,598 results for: pfas

Showing: 781 - 800 of 1598 results

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pFastBac StrepII msfGFP TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-Rgfp)

Plasmid

#55221

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

StrepII and msfGFP

Expression

Insect

Mutation

Promoter

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V1)

Plasmid

#55222

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

SNAPf and TwinStrepII

Expression

Insect

Mutation

Promoter

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V3)

Plasmid

#55223

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

SNAPf and TwinStrepII

Expression

Insect

Mutation

Promoter

Available since

July 9, 2014

Availability

Academic Institutions and Nonprofits only

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pFGAMS-EGFP

Plasmid

#99107

Purpose

Expresses human PFAS-EGFP fusion in mammalian cells

Depositor

Stephen Benkovic

Article

An et al Science. 2008 Apr 4;320(5872):103-6. doi

Insert

PFAS (PFAS Human)

Use

Tags

EGFP (enhanced green fluorescent protein)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pFGAMS-mCherry

Plasmid

#166024

Purpose

Expresses human PFAS-mCherry fusion in mammalian cells

Depositor

Stephen Benkovic

Article

Doigneaux et al J Biol Chem. 2020 Jul 10;295(28):9551-95

Insert

PFAS-mCherry (PFAS Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pFGAMS-mMaple3

Plasmid

#154912

Purpose

Expresses human PFAS-mMaple3 fusion in mammalian cells

Depositor

Stephen Benkovic

Article

Chan et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

PFAS (PFAS Human)

Use

Tags

mMaple3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pFGAMS-mEos2

Plasmid

#105128

Purpose

Expresses human FGAMS-mEos2 fusion in mammalian cells

Depositor

Stephen Benkovic

Article

French et al Science. 2016 Feb 12;351(6274):733-7. do

Insert

PFAS (PFAS Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCCM

Plasmid

#162708

Purpose

Second CCM operon cloned from H. neapolitanus; pFA expressing the remaining 11 genes in the H. neapolitanus CCM cluster

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

Second CCM operon cloned from H. neapolitanus

Use

Tags

Expression

Bacterial

Mutation

Promoter

PLtet0-1 promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-ProtC-FLAG-AU1

Plasmid

#162097

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to ProtC-FLAG-AU1

Use

Lentiviral

Tags

ProtC-FLAG-AU1

Expression

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3mC_Puro-T2A-mCherry-ProtC-FLAG-AU1

Plasmid

#162117

Purpose

mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; mCherry linked to ProtC-FLAG-AU1

Use

Lentiviral

Tags

ProtC-FLAG-AU1

Expression

Mutation

WT

Promoter

EF1A

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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ER-FAST

Plasmid

#216340

Purpose

For testing localization of pFAST protein to endoplasmic reticulum

Depositor

Alison Tebo

Insert

PRL_pFAST

Use

Tags

myc for FAST protein

Expression

Mammalian

Mutation

n/a

Promoter

CMV

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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Peroxisomes-FAST

Plasmid

#216343

Purpose

For testing localization of pFAST protein to peroxisomes

Depositor

Alison Tebo

Insert

pFAST_SKL

Use

Tags

myc for FAST protein

Expression

Mammalian

Mutation

n/a

Promoter

CMV

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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Golgi-FAST

Plasmid

#216341

Purpose

For testing localization of pFAST protein to Golgi complex

Depositor

Alison Tebo

Insert

ST6Gal1_pFAST

Use

Tags

myc for FAST protein

Expression

Mammalian

Mutation

n/a

Promoter

CMV

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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Mito-FAST

Plasmid

#216342

Purpose

For testing localization of pFAST protein to mitochondria

Depositor

Alison Tebo

Insert

Cox8_pFAST

Use

Tags

myc for FAST protein

Expression

Mammalian

Mutation

n/a

Promoter

CMV

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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Peroxisomes-FAST-IRES-iRFP670

Plasmid

#216347

Purpose

For testing localization of pFAST and iRFP670 proteins to peroxisomes

Depositor

Alison Tebo

Insert

pFAST_SKL-IRES-iRFP670_PXMP2

Use

Tags

myc for FAST protein only

Expression

Mammalian

Mutation

n/a

Promoter

CMV

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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Mito-FAST-IRES-iRFP670

Plasmid

#216346

Purpose

For testing localization of pFAST and iRFP670 proteins to mitochondria

Depositor

Alison Tebo

Insert

Cox8_pFAST-IRES-Tomm20_iRFP670

Use

Tags

myc for FAST and iRF670 proteins

Expression

Mammalian

Mutation

n/a

Promoter

CMV

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ER-FAST-IRES-iRFP670

Plasmid

#216344

Purpose

For testing localization of pFAST and iRFP670 proteins to endoplasmic reticulum

Depositor

Alison Tebo

Insert

PRL_pFAST-IRES-CANX_iRFP670

Use

Tags

myc for FAST and iRF670 proteins

Expression

Mammalian

Mutation

n/a

Promoter

CMV

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Golgi-FAST-IRES-iRFP670

Plasmid

#216345

Purpose

For testing localization of pFAST and iRFP670 proteins to Golgi complex

Depositor

Alison Tebo

Insert

ST6Gal1_pFAST-IRES-Sys1_iRFP670

Use

Tags

myc for FAST and iRF670 proteins

Expression

Mammalian

Mutation

n/a

Promoter

CMV

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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2C::tdTomato Reporter

Plasmid

#40281

Depositor

Samuel Pfaff

Article

Macfarlan et al Nature. 2012 Jul 5;487(7405):57-63.

Insert

2C Promoter (2-cell stage promoter)

Use

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only

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betaRBPIG

Plasmid

#16422

Depositor

Juergen Hescheler

Article

Pfannkuche et al Nat Protoc. 2008. 3(9):1510-26. doi: htt

Insert

loxP flanked RFP and blasticidin-resistance plus puromycin-acetyltransferase IRES GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only