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Addgene

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We narrowed to 28,546 results for: ple

Showing: 781 - 800 of 28546 results

  6. TRUPATH Triple GaoA

    Plasmid
    #196051
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaoA-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196051-plasmid-map-sequence-id-385865

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  8. pLenti6.2-ccdB-Nanoluc

    Plasmid
    #87075
    Purpose
    Lentiviral Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase
    Depositor
    Mikko Taipale
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Nanoluc luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    87075-plasmid-map-sequence-id-215540

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  10. pLEX307-APN-puro

    Plasmid
    #158454
    Purpose
    Constitutive lentiviral expression of human APN.
    Depositor
    Alejandro Chavez, Sho Iketani
    Insert
    APN (ANPEP Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158454-plasmid-map-sequence-id-308145

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  12. plex_307-ccdB-g418

    Plasmid
    #201141
    Purpose
    pLEX_307 plasmid with g418 marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201141-plasmid-map-sequence-id-395514

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  15. pLenti-PE2-BSD

    Plasmid
    #161514
    Purpose
    Expression of PE2 in mammalian cells. Can be used to generate stable cell lines by Blasticidin selection
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Sep 21. pii: 10.103
    Insert
    PE2-P2A-BSD
    Use
    Lentiviral
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α core promoter
    Available since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161514-plasmid-map-sequence-id-317692

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  17. pLenti-nSpCas9-APOBEC3A

    Plasmid
    #209040
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC3A is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    APOBEC3A-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209040-plasmid-map-sequence-id-413592

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  18. TRUPATH Triple GaZ

    Plasmid
    #196053
    Purpose
    Encodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaz-RLuc8 (GNAZ Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196053-plasmid-map-sequence-id-387469

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Showing: 781 - 800 of 28546 results