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Showing: 781 - 800 of 806 results

  7. pNOC_dCas9_sgRNA Td2

    Plasmid
    #176258
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Expression
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176258-plasmid-map-sequence-id-347041

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  8. pNOC_dCas9_sgRNA Td1

    Plasmid
    #176257
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 1
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176257-plasmid-map-sequence-id-347030

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  9. pNOC_dfnCas12a_crRNA Td2

    Plasmid
    #176255
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Expression
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176255-plasmid-map-sequence-id-347038

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  12. AAV-short_BrokenHeart (no BC)

    Plasmid
    #86950
    Purpose
    AAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.
    Depositor
    Joseph Dougherty, Rob Mitra
    Article
    Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    Brokenheart construct
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    86950-plasmid-map-sequence-id-172437

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  14. pNOC_dCas9-KRAB_sgRNA Td2

    Plasmid
    #176264
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Expression
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176264-plasmid-map-sequence-id-347050

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  15. pNOC_dfnCas12a-KRAB_crRNA Td2

    Plasmid
    #176261
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Expression
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176261-plasmid-map-sequence-id-346586

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  20. pAAV-hDlx-Flex-dTomato-Fishell_7

    Plasmid
    #83894
    Purpose
    Cre recombinase-dependent dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, and AAV9
    Insert
    tdTomato
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hDlx
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    83894-plasmid-map-sequence-id-163144

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Showing: 781 - 800 of 806 results