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pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)

Plasmid

#196703

Purpose

Plasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.

Depositor

Karlene Cimprich

Article

Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

Insert

human RNaseH1 D210N

Expression

Mammalian

Mutation

D210N, catalytically inactive

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-RAD52-WT

Plasmid

#235676

Purpose

Expresses HA-tagged wild type human RAD52 in mammalian cells.

Depositor

Simon Powell

Article

Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

Insert

RAD52 (RAD52 Human)

Tags

HA

Expression

Mammalian

Mutation

wild type

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet3

Plasmid

#189754

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet3 red fluorescent protein

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Arf6 DN T27N

Plasmid

#10831

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf6 T27N (ARF6 Human)

Tags

HA

Expression

Mammalian

Mutation

T27N. Defective in GTP binding. Dominant negati…

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-FCHO1

Plasmid

#196910

Purpose

visualization of clathrin-coaed vesicle formation in living mammalian cells

Depositor

Kenji Sugimoto

Article

Sakaushi et al Biosci Biotechnol Biochem. 2007 Jul;71(7

Insert

FCHO1 (FCHO1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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Lamp1_mStayGold

Plasmid

#221902

Purpose

Expresses mStayGold fused to Lamp1

Depositor

Janelia Integrative Imaging

Insert

Lamp1_mStayGold

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pT2/LTR7-GFP

Plasmid

#62541

Purpose

LTR7-driving reporter

Depositor

Zsuzsanna Izsvak

Article

Wang et al Nature. 2014 Dec 18;516(7531):405-9. doi

Insert

LTR7-GFP

Use

Sleeping beauty transposon

Expression

Mammalian

Promoter

LTR7

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-TadA8e-PcrA

Plasmid

#221228

Purpose

Express HACE Editor as a fusion of TadA-8e adenosine deaminase and PcrA helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

TadA8e-PcrA

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5F-TACE

Plasmid

#31713

Purpose

Mammalian expression of human TACE (ADAM17)

Depositor

Rik Derynck

Article

Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

Insert

TACE (ADAM17 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 13, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pFREE

Plasmid

#92050

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Article

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Inserts

Cas9
gRNA array

Use

CRISPR

Expression

Bacterial

Promoter

pRhamBAD and ptet

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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