164,577 results

1. p20StAD

   Plasmid

   #190268

   Purpose

   Constitutively expresses a protein of interest fused to a GAL4 transcription activating domain. Targeted integration to genomic locus YPRC-delta15, Chromosome XVI.

   Depositor

   Assaf Mosquna

   Article

   Zimran et al ACS Synth Biol. 2022 Aug 19;11(8):2880-2

   Type

   Empty backbone

   Use

   Synthetic Biology; Yeast genome-integrating vector

   Tags

   GAL4-AD

   Expression

   Mutation

   Promoter

   TDH3

   Available Since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCytERM-mTagBFP2

   Plasmid

   #184940

   Purpose

   ER Visualization (OSER assay) Expresses mTagBFP2 in fusion with N-terminal part of p450

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   CytERM-mTagBFP2
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEBTet-SNAP-Cep72

   Plasmid

   #136819

   Purpose

   Mammalian expression of the centrosomal protein Cep72 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-Cep72 (CEP72 Human, Synthetic)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO_H2B-HaloTag11-T2A-EGFP

   Plasmid

   #175522

   Purpose

   Nuclear expression of HaloTag11 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag11
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag11 = HaloTag7-M175W

   Promoter

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSOX11-donor

   Plasmid

   #176360

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Kevin White, Barbara Stranger

   Insert

   SOX11 (SOX11 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-hfYFP

   Plasmid

   #186523

   Purpose

   Hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   Hyperfolder YFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-C2-NLRP3

   Plasmid

   #73955

   Purpose

   Express EGFP-tagged human NLRP3 in mammalian cells

   Depositor

   Christian Stehlik

   Article

   Khare et al Immunity. 2012 Mar 23;36(3):464-76. doi:

   Insert

   NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV iE

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACUH-GFP1-10 mitochondrial matrix

   Plasmid

   #172063

   Purpose

   UAS vector to express GFP1-10 in the mitochondrial matrix

   Depositor

   Daichi Kamiyama

   Article

   Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   GFP1-10 mitochondrial matrix
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-2xFLAG-2xSTREP-CCND2

   Plasmid

   #172629

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged cyclin D2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND2 (CCND2 Human)
   

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pelB-MBP-HtrA-ss

    Plasmid

    #137066

    Purpose

    E. coli expression clone (T7lac promoter) for mature HtrA (aa 28-474) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
    

    Use

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    mature HtrA: lacks the HtrA signal peptide

    Promoter

    T7lac

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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