166,289 results

1. pTXB1-pA-M.EcoGII

   Plasmid

   #192873

   Purpose

   pA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578

   Depositor

   Chong Tang

   Article

   Weng et al Genome Biol. 2023 Mar 29;24(1):61. doi:

   Insert

   pA-M.EcoGII
   

   Tags

   3X Flag Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEM705 Gsi (Ser)

   Plasmid

   #109352

   Purpose

   GsX chimera with Gαi C-terminus (Pertussis toxin insensitive mutant)

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Gsi chimera
   

   Tags

   None

   Expression

   Mammalian

   Mutation

   Changed Cys at position 391 to Serine (Pertussis …

   Promoter

   CAG

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA5-FRT/TO-3*Flag-APEX2 N-term NONO

   Plasmid

   #187580

   Purpose

   Express FLAG epitope and APEX2-tagged NONO fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   NONO (NONO Human)
   

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

   Plasmid

   #221235

   Purpose

   Tet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA
   

   Use

   Lentiviral

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pT7-EGFP-C1-HsEDC4_S

   Plasmid

   #147455

   Purpose

   Mammalian Expression of HsEDC4. Please note that this plasmid does not contain the T7 promoter.

   Depositor

   Elisa Izaurralde

   Article

   Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

   Insert

   HsEDC4 (EDC4 Human)
   

   Expression

   Mammalian

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDH_Str-Ii

   Plasmid

   #65313

   Purpose

   Cytoplasmic ER hook only (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Invariant chain (p33)
   

   Use

   Lentiviral

   Mutation

   Streptavidin in its cytoplasmic part

   Promoter

   CMV

   Available Since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV.hSyn.eGFP.WPRE.bGH

   Plasmid

   #105539

   Purpose

   AAV expression of EGFP from hSyn promoter

   Depositor

   James M. Wilson

   Has Service

   AAV1

   Insert

   EGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pf hPGK mc2 IRES puro

   Plasmid

   #206237

   Purpose

   For expressing cloned exons as circular RNA.

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUK21

   Plasmid

   #49788

   Purpose

   E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)

   Depositor

   Joachim Messing

   Article

   Vieira et al Gene. 1991 Apr;100:189-94.

   Type

   Empty backbone

   Use

   Cloning vector

   Promoter

   lacZ

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDual-IN-SMN1

    Plasmid

    #215721

    Purpose

    dual-color reporter express eGFP constitutively ; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7; expresses SMN1

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Insert

    SMN1 EXON 7 (SMN1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge