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pJUMP39-1A(sfGFP)

Plasmid

#126983

Purpose

Level 1 vector with alternative selection marker (chloramphenicol). OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a- mECitrine-kanMX6

Plasmid

#105150

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

Insert

mECitrine-kanMX6

Tags

mECitrine

Expression

Bacterial and Yeast

Mutation

F64L, Q69M, A206K

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pJH807_pJH_LldR_Pcon-sfGFP.2TAG

Plasmid

#163738

Purpose

Constitutive expression of green fluorescent protein encoding two stop codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.2TAG

Expression

Bacterial

Mutation

gfp.N39TAG,Y135TAG

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH809_pJH_LldR_Pcon-sfGFP.10TAG

Plasmid

#163739

Purpose

Constitutive expression of green fluorescent protein encoding ten stop codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.10TAG

Expression

Bacterial

Mutation

gfp with first 10 leucine codons mutated to amber

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB8382

Plasmid

#126916

Purpose

Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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IDP431

Plasmid

#86999

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

CLIP-Nrx1B-4xλN(i)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pRS316Nab3

Plasmid

#110533

Purpose

Expresses yeast NAB3 using the endogenous promoter

Depositor

Daniel Reines

Article

Loya et al Nucleic Acids Res. 2012 Aug;40(15):7476-

Insert

NAB3 (NAB3 Budding Yeast)

Expression

Yeast

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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IDP455

Plasmid

#87001

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

barcode landing site Gfp-4xboxB

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pJUMP27ts-1A(lacZ)

Plasmid

#126977

Purpose

Level 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

RepA A56V thermosensitive mutation

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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SEPT11 sh1 3m shRNA

Plasmid

#122021

Purpose

Negative control shRNA for SEPT11 sh1 shRNA (plasmid 122020).

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 shRNA (Sept11 Rat)

Use

RNAi

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pZA71

Plasmid

#174785

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_synthesized NbJIM2 (NbJIM2syn)-3xFLAG, Kmr plant selection marker

Tags

3xFLAG

Expression

Plant

Mutation

WT

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pZA68

Plasmid

#174781

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E/D481V-eGFP, Kmr plant selection marker

Tags

eGFP

Expression

Plant

Mutation

L17E/D481V

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pZA66

Plasmid

#174779

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-D481V-eGFP, Kmr plant selection marker

Tags

eGFP

Expression

Plant

Mutation

D481V

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pZA55

Plasmid

#174768

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E without STOP codon

Use

Unspecified

Mutation

L17E

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pZA56

Plasmid

#174769

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E/D481V without STOP codon

Use

Unspecified

Mutation

L17E/D481V

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pZA54

Plasmid

#174767

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-D481V without STOP codon

Use

Unspecified

Mutation

D481V

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pZA53

Plasmid

#174766

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn without STOP codon

Use

Unspecified

Mutation

WT

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pZA65

Plasmid

#174778

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-eGFP, Kmr plant selection marker

Tags

eGFP

Expression

Plant

Mutation

WT

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pZA67

Plasmid

#174780

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E-eGFP, Kmr plant selection marker

Tags

eGFP

Expression

Plant

Mutation

L17E

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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AtFLS2pro::GUS

Plasmid

#87167

Purpose

expression pattern of AtFLS2

Depositor

Silke Robatzek

Article

Beck et al J Exp Bot. 2014 Dec;65(22):6487-98. doi:

Insert

AT5G46330 promoter

Tags

GUS

Promoter

AtFLS2

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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