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DCT.VV.Orf66

Plasmid

#12754

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf66

Expression

Mammalian

Available Since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf36

Plasmid

#12724

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf36

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf47

Plasmid

#12735

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf47

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf54

Plasmid

#12742

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf54

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
pIRES:hTRAAK(W262S)

Plasmid

#133078

Purpose

Mammalian expression vector. It will generate the human TRAAK channel full lenght W262S mutant

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

Potassium channel subfamily K member 4 (KCNK4, TRAAK) W262S (KCNK4 Human)

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pIRES:hTRAAK(G124I)

Plasmid

#133079

Purpose

Mammalian expression vector. It will generate the human TRAAK channel full lenght G124I mutant

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

Potassium channel subfamily K member 4 (KCNK4, TRAAK) G124I (KCNK4 Human)

Expression

Mammalian

Mutation

G124I

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cerulean-Epac2(R53A)-Venus

Plasmid

#138197

Purpose

FRET-based Epac2 activation sensor with R53A mutation in Epac2 coding sequence.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2(R53A)-Venus (Rapgef4 Mouse, Synthetic)

Tags

Cerulean and Venus

Expression

Mammalian

Mutation

Contains arginine-to-alanine substitution at resi…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cerulean-Epac2(R447)-cpV[E172]

Plasmid

#138198

Purpose

FRET-based Epac2 activation sensor with R447A mutation in Epac2 coding sequence.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2(R447A)-cpVenus[E172] (Rapgef4 Mouse, Synthetic)

Tags

Cerulean and cpV[E172]

Expression

Mammalian

Mutation

Contains arginine-to-alanine substitution at resi…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cerulean-Epac2(R466A)-Venus

Plasmid

#138199

Purpose

FRET-based Epac2 activation sensor with R466A mutation in Epac2 coding sequence.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2(R466A)-Venus (Rapgef4 Mouse, Synthetic)

Tags

Cerulean and Venus

Expression

Mammalian

Mutation

Contains arginine-to-alanine substitution at resi…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cerulean-Epac2(L426W)-Venus

Plasmid

#138200

Purpose

FRET-based Epac2 activation sensor with L426W mutation in Epac2 coding sequence.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2(L426W)-Venus (Rapgef4 Mouse, Synthetic)

Tags

Cerulean and Venus

Expression

Mammalian

Mutation

Contains leucine-to-tryptophan substitution at re…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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5013 TRV2-eTnpBc-reRNA4ChlH

Plasmid

#251824

Purpose

Editing H subunit of magnesium chelatase (ChlH) genes in Nicotiana benthamiana

Depositor

Savithramma Dinesh-Kumar

Article

Nagalakshmi et al Nat Plants. 2026 Mar;12(3):503-511. doi:

Insert

Enhanced variant of ISDra2 eTnpBc with guide targeting NbChlH with HDV at 3' end

Tags

AtFDnls

Expression

Plant

Mutation

N4Y/R110K/V192L/L222I

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pLV_3xFLAG-ATP6V0A3-mScarlet

Plasmid

#228867

Purpose

Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG and C-terminal mScarlet

Depositor

Wilhelm Palm

Article

Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

Insert

ATP6V0A3 (TCIRG1 Human)

Use

Lentiviral

Tags

3xFLAG and mScarlet

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-SRRM2-mCherry

Plasmid

#174089

Purpose

Inducibly expresses SRRM2-mCherry in mammalian cells with the Tet-on system

Depositor

Wesley Sundquist, Katie Ullman

Article

Williams et al Elife. 2021 Aug 4;10. pii: 63743. doi: 1

Insert

SRRM2 (SRRM2 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

TREtight

Available Since

Sept. 12, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-NLS-R-GECO

Plasmid

#32462

Purpose

Mammalian expression of nucleus-targeting red fluorescent genetically encoded Ca2+ indicator for optical imaging

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

R-GECO1.0

Tags

NLS sequence DPKKKRKV

Expression

Mammalian

Mutation

Substitutions relative to the mApple-derived anal…

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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p(HA)HIF1alpha (P402A,P564A)

Plasmid

#52636

Purpose

mammalian expression of HA tagged HIF1alpha (full length) with P402A,P564A mutation

Depositor

Eric Huang

Article

Kageyama et al FASEB J. 2004 Jun;18(9):1028-30. Epub 20

Insert

HIF1alpha P402A, P564A (HIF1A Human)

Tags

HA

Expression

Mammalian

Mutation

P402A, P564A

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLV_3xFLAG-ATP6V0A3

Plasmid

#228866

Purpose

Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG

Depositor

Wilhelm Palm

Article

Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

Insert

ATP6V0A3 (TCIRG1 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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PGK-DTAG-CTERM-GFP

Plasmid

#185770

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, dTAG

Mutation

none

Promoter

PGK

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV/D377Y-mPCSK9

Plasmid

#58376

Purpose

Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

Depositor

Jacob Bentzon

Article

Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

Has Service

AAV8

Insert

proprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)

Use

AAV

Expression

Mammalian

Mutation

D377Y

Promoter

HCRApoE/hAAT

Available Since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pSP64-mHCN2

Plasmid

#53060

Purpose

Expresses alpha subunit of mouse HCN2 channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Chen et al J Biol Chem. 2000 Nov 17;275(46):36465-7

Insert

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (Hcn2 Mouse)

Use

Xenopus oocyte expression

Mutation

E55G, R237H, and K283R polymorphisms compared to …

Promoter

SP6

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 DNAJB1

Plasmid

#19522

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1 Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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