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MS2-adRNA (3'UAG)

Plasmid

#170128

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(3'UAG)-MS2

Use

Lentiviral

Expression

Mammalian

Promoter

Human U6 and mouse U6

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-hRIP3-FL V460P

Plasmid

#61379

Purpose

Expresses Human full length RIPK3 with V460P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with V460Pmutation (RIPK3 Human)

Tags

EYFP

Expression

Mammalian

Mutation

changed V460 to P

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pEYFP-N1-hRIP3-FL V458P

Plasmid

#61377

Purpose

Expresses Human full length RIPK3 with V458P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with V458Pmutation (RIPK3 Human)

Tags

EYFP

Expression

Mammalian

Mutation

changed V458 to P

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmCup_C

Plasmid

#146041

Purpose

Insect Expression of DmCup

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmCup (cup Fly)

Expression

Insect

Mutation

P418S

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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Ets2DBM

Plasmid

#28129

Depositor

Pumin Zhang

Article

Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

Insert

Ets2 (ETS2 Human)

Use

Mouse Targeting and Retroviral

Expression

Mammalian

Mutation

The putative destruction box (RGTL) residues 20–2…

Available Since

April 12, 2011

Availability

Academic Institutions and Nonprofits only
pILGFP86

Plasmid

#83543

Purpose

Used to evaluate the expression output of RPL4A promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

RPL4A promoter-yEGFP

Expression

Yeast

Promoter

RPL4A

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-mCE(K294A)

Plasmid

#82461

Purpose

Expression of myc epitope and catalytic inactive form of murine mRNA Capping Enzyme (K294A) in mammalian cells

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

mCE (K294A) (Rngtt Mouse)

Tags

myc

Expression

Mammalian

Mutation

Lysine 294 changed to Alanine

Promoter

CMV

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pUPD_OsU3 (GB1184)

Plasmid

#68209

Purpose

Provides the O. sativa U3 RNA polIII promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

OsU3 promoter

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pHH0103_NCK1-1/3

Plasmid

#91472

Purpose

Protein expression and purification of human SH3 domain construct NCK1-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCK1-1/3 (NCK1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO DNAJB7

Plasmid

#19476

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB7 (DNAJB7 Human)

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0112

Plasmid

#117548

Purpose

Level 1 Golden Gate Cassette: xCas9 3.7 expression cassette for dicotyledonous plants

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

2x35S+5'UTR OMEGA (pICH51288) + Sp-xCas9 3.7 (no stop codon) (pEPOR0SP0011) + YFP (pICSL50005) + Nos (pICH41421)

Expression

Plant

Promoter

2x35S+5'UTR OMEGA (pICH51288)

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO DNAJB8

Plasmid

#19477

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB8 (DNAJB8 Human)

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1X-Nup88-mut

Plasmid

#87337

Purpose

To express human Nup88 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP88 (NUP88 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ERKsubDD-Venus-NES

Plasmid

#84630

Purpose

Encodes C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

ERKsubDD-Venus-NES

Tags

6xHis, Nuclear export signal (NES), T7 tag (gene …

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pPAct2noUTR (GB0192)

Plasmid

#68177

Purpose

Provides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtAct2 promoter (no UTR)

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pHH0103_SHANK1-1/1

Plasmid

#91517

Purpose

Protein expression and purification of human SH3 domain construct SHANK1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SHANK1-1/1 (SHANK1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-CC2

Plasmid

#51221

Purpose

expression of amino acid 917-1040 of chicken DCTN1 p150Glued in mammalian cells

Depositor

Trina Schroer

Article

Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

Insert

DCTN1 (DCTN1 Chicken)

Expression

Mammalian

Mutation

aa917-1040

Promoter

CMV

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmCaf40

Plasmid

#79251

Purpose

Expresses EGFP-tagged DmCaf40 in S2 cells

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCaf40 (Rcd-1 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pHH0103_NCK2-3/3

Plasmid

#91256

Purpose

Protein expression and purification of human SH3 domain construct NCK2-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCK2-3/3 (NCK2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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Vh-HA-FRB

Plasmid

#20225

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

FRB (MTOR Human)

Tags

HA and Vh

Expression

Mammalian

Mutation

T2098L mutation

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only