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PiggyBac EF1a-eGFP-Puro U6 CLCN3 g1

Plasmid

#170828

Purpose

PiggyBac Cas13d sgRNA plasmid for CLCN3 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d CLCN3 gRNA1

Use

Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 CLCN3 g2

Plasmid

#170829

Purpose

PiggyBac Cas13d sgRNA plasmid for CLCN3 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d CLCN3 gRNA2

Use

Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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FGF8-P2A-iCre

Plasmid

#210800

Purpose

Recombinase reporter for FGF8 expression

Depositor

Yuejun Chen

Article

You et al Cell Stem Cell. 2023 Apr 6;30(4):473-487

Insert

FGF8 (FGF8 Human)

Use

Cre/Lox

Tags

iCre

Expression

Mammalian

Promoter

endougeneous FGF8 promoter

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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APCDD1-P2A-tdTomato

Plasmid

#210801

Purpose

Fluorescent reporter for APCDD1 expression

Depositor

Yuejun Chen

Article

You et al Cell Stem Cell. 2023 Apr 6;30(4):473-487

Insert

APCDD1 (APCDD1 Human)

Tags

tdtomato

Expression

Mammalian

Promoter

endougeneous APCDD1 promoter

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLCHKOv3-mClover3

Plasmid

#209034

Purpose

Lentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites, mClover3 and puromycin selection marker

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ7578 pHR (hU6-crPROX-EFS-PuroR-WPRE)

Plasmid

#214882

Purpose

Lentiviral vector encoding RfxCas13d targeting PROX guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crPROX-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti4/V5-DEST-AERRIE

Plasmid

#204023

Purpose

Lentiviral vector expressing human long non-coding RNA AERRIE (linc01013)

Depositor

Reinier Boon

Article

Pham et al Front Cell Dev Biol. 2021 Jan 11;8:61907

Insert

long non-coding RNA AERRIE (LINC01013 Human)

Use

Lentiviral

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV puro PRDM14 ΔSET domain

Plasmid

#193680

Purpose

Constitutive retroviral expression of PRDM14

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

PRDM14 (PRDM14 Human)

Use

Retroviral

Mutation

Deletion of SET domain

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV puro PRDM14 ΔZinc finger domain

Plasmid

#193679

Purpose

Constitutive retroviral expression of PRDM14

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

PRDM14 (PRDM14 Human)

Use

Retroviral

Mutation

Deletion of Zinc finger domain

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV puro PRDM14

Plasmid

#193677

Purpose

Constitutive retroviral expression of PRDM14

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

PRDM14 (PRDM14 Human)

Use

Retroviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgINTS12 guide 2

Plasmid

#193607

Purpose

INTS12 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgINTS12 guide 2 (INTS12 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgINTS12 guide 1

Plasmid

#193606

Purpose

INTS12 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgINTS12 guide 1 (INTS12 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgRELA guide 2

Plasmid

#193592

Purpose

RELA knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgRELA guide 2 (RELA Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV puro PRDM14 ΔSET+ΔZinc finger domains

Plasmid

#193678

Purpose

Constitutive retroviral expression of PRDM14

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

PRDM14 (PRDM14 Human)

Use

Retroviral

Mutation

Deletion of SET and Zinc finger domains

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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PLL5.0-Anillin ShRNA-GFP-AnillinA740D, E758K

Plasmid

#187274

Purpose

Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin A740D,E758K

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)

Use

Lentiviral

Tags

EGFP

Mutation

Anillin A740D, E758K

Promoter

U6, 5'LTR

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-mDia1-GBD (1-305 aa) in pEGFPN1

Plasmid

#187280

Purpose

Express EGFP-mDia1 GTPase-binding domain

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

mDia1 (Diaph1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

GBD (1-305aa)

Promoter

CMV

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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IL2-mCherry-rGBD in pmCherryN1

Plasmid

#187286

Purpose

Express IL2-Rhotekin GTPase-binding domain-mCherry

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit, Rhotekin (IL2, Rtkn Human, Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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piwi_sgRNA

Plasmid

#186653

Purpose

piwi sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Piwi sgRNA Plasmid (piwi Fly)

Expression

Insect

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_C_FH_Nbr

Plasmid

#186662

Purpose

Donor plasmid to endogenously tag Nbr gene in Drosophila melanogaster at the C-terminal.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Nbr C-terminal 3xFlag-3xHA Tag Donor Plasmid (Nbr Fly)

Tags

3xFlag-3xHA

Expression

Insect

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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Nbr_C_sgRNA2

Plasmid

#186664

Purpose

Nbr C-tag sgRNA2 plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Nbr sgRNA 2 Plasmid (Nbr Fly)

Expression

Insect

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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