We narrowed to 25,310 results for: expression vector
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Plasmid#107179PurposeExpression of N-terminal fused Kir7.1 in mammalian cells.DepositorInsertKCNJ13 (KCNJ13 Human)
UseTagsRFPExpressionMammalianMutationPromoterhACTBAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVP16-tev-PRT1
Plasmid#83405Purposebacterial expression vector pVP16 containing Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studiesDepositorInsertPROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
UseTagsHis8, MBP, and tev cleavage siteExpressionBacterialMutationPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pRDA_852
Plasmid#185527PurposeLentiviral vector that enables Cre-mediated expression of two sgRNAs. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.DepositorInsertsFirst sgRNA cassette
Second sgRNA cassette
UseCRISPR, Cre/Lox, and Lentiviral ; Flp/frtTagsExpressionMammalianMutationPromoterHuman U6 and Mouse U6Available sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR/hBIG1
Plasmid#226251PurposeEntry plasmid of hBIG1 for Gateway systemDepositorInsertBIG1 (ARFGEF1 Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmVen-IRES
Plasmid#211428PurposeVector backbone for coupled mammalian expression of N-terminal Venus fusion protein and another proteinDepositorInsertIRES sequence (RPA2 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-Emerald-WPRE-UbC-MCS
Plasmid#211800PurposeLentiviral vector plasmid expressing Emerald under the spleen focus-forming virus (SFFV) promoter and containing a multiple cloning site (MCS) under the Ubiquitin C (UbC) promoterDepositorInsertsEmerald
multiple cloning site
UseLentiviralTagsExpressionMammalianMutationPromoterSFFV and UbCAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHygG6
Plasmid#200219PurposehygR selection GFP marked rapid RMCE integration vectorDepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
ppie.001.301.W257A
Plasmid#137652PurposeExpresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.DepositorInsertppie (PPIE Human)
UseTagsGST-(His)6-thrombinExpressionBacterialMutation001-301-W257APromoterAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
PRPF4.001.522.K45A.pc4
Plasmid#137648PurposeExpresses nuclear localization signal (NLS), C-terminal myc-(His)6 fusion of human gene or portion of gene in human cell culture lines. pcDNA4.1-based vector.DepositorInsertPRPF4 (PRPF4 Human)
UseTagsNLS and myc-(His)6ExpressionMammalianMutation001-522-K45A-pc4PromoterAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
PRPF4.001.522.Y32A.pc4
Plasmid#137646PurposeExpresses nuclear localization signal (NLS), C-terminal myc-(His)6 fusion of human gene or portion of gene in human cell culture lines. pcDNA4.1-based vector.DepositorInsertPRPF4 (PRPF4 Human)
UseTagsNLS and myc-(His)6ExpressionMammalianMutation001-522-Y32A-pc4PromoterAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSDest-ENTR-MCP-ADAR2-DD(E488Q/T490A)-V5
Plasmid#192714PurposeMammalian expression vector containing MS2 coat protein fused to ADAR2 catalytic domain (E488Q/T490A) with V5 tagDepositorInsertMCP-ADAR2-DD(E488Q/T490A)-V5
UseTagsV5ExpressionMammalianMutationE488Q/T490APromoterCMVAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
mApple_pcDNA3.1+
Plasmid#182855PurposeMonomer expression vectorDepositorInsertmApple
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBSIIKS_HIS3_I
Plasmid#109062Purposeyeast shuttle vector with deletable CEN6-ARSH4 cassette in pBIIKS backbone. LoxP site upstream of HIS3. For teaching purposes.DepositorInsertHIS3 (HIS3 Budding Yeast, Zygosaccharomyces rouxii)
UseCre/LoxTagsExpressionBacterial and YeastMutationPromoterHIS3Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUPD2b:TMV-3NTR
Plasmid#106218PurposeProvides Nos terminator and 3' part of Tobacco mosaic virus (3NTR, ribozyme) as level 0 GoldenBraid part (GCTT-CGCT)DepositorInsertInfectious clone of Tobacco mosaic virus, 3' part
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Plekhm1 -PH1/2 pGEX 4T2-GST
Plasmid#73831PurposeExpress human Plekhm1 (PH1 and PH2 domains) in bacterial cellsDepositorInsertPlekhm1 (PLEKHM1 Human)
UseTagsGstExpressionBacterialMutationto vector was cloned Ph1 and Ph2 domains of human…PromoterAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pST44
Plasmid#64007Purposepolycistronic cloning vector for pST44 plasmid suiteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTIN
Plasmid#162885PurposeOverexpression vector for the fission yeast Schizosaccharomyces pombeDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-ActA-IRES-puromycin-pLVx-EF1a
Plasmid#177433PurposeTo express mCherry fused to ActA mitochondria targeting sequence. Lentiviral vector used to make cell lines expressing this mitochondria landmark.DepositorInsertActA tail anchor (actA Listeria monocytogenes)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[]:nnLuz-I{PIV-216}:NosT
Plasmid#212187PurposeThis binary vector expresses mushroom luciferase (nnLuz) containing the PIV intron (216 bp into the gene) with the truncated 35sCAMV promoterDepositorInsertnnLuz-I{PIV-216}
UseTagsExpressionPlantMutationPIV intron added at bp 216, eliminates expression…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only