We narrowed to 24,878 results for: promoter

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pL-CRISPR.EFS.PAC

Plasmid

#57828

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-PAC

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro CXCR4 siRNA-1

Plasmid

#12271

Depositor

Bob Weinberg

Article

Orimo et al Cell. 2005 May 6. 121(3):335-48.

Insert

CXCR4 siRNA (CXCR4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On shYap1-4

Plasmid

#193670

Purpose

Tet inducible knockdown of Yap1

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

Yap1 (Yap1 Mouse)

Use

Lentiviral

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-CD86-mTFP1

Plasmid

#223606

Purpose

Lentiviral expression of human CD86 with mTFP1 tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

mTFP1

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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VRK1-dTag-puro

Plasmid

#199651

Purpose

Expresses VRK1 C-term. Tagged with dTAG

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

VRK1 (VRK1 Human)

Use

Lentiviral

Tags

dTAG

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_FH-AGO2-WT

Plasmid

#92006

Purpose

Expresses FLAG-HA-AGO2 (WT)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Tags

FLAG-HA

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV p2GM AMPK alpha2hp1 alpha1hp1

Plasmid

#89492

Purpose

shRNA against AMPK alpha2 and alpha1 in mouse/human to knock down protein expression

Depositor

Russell Jones

Article

Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi

Insert

2 hairpins against mouse/human AMPKalpha1 and alpha2

Use

Retroviral

Expression

Mammalian

Promoter

U6 (The GFP-Puro is from a PGK promoter; the shRN…

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only
pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)

Plasmid

#64217

Purpose

Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
hU6 promoter; BbsI sites for sgRNA
H1 promoter; BamHI site for shRNA

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-mCherry

Expression

Mammalian

Promoter

CBh, H1, and U6

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pLX317-RBFOX1

Plasmid

#115442

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

RBFOX1 (RBFOX1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α P405A Δ450-572-pBabe-Puro

Plasmid

#25958

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 KBTBD4 R313PRR

Plasmid

#184629

Purpose

Mammalian expression vector with N-terminal Flag for KBTBD4 R313PRR expression in mammalian cells

Depositor

Vincenzo D'Angiolella

Article

Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

Insert

N-terminal 3xFlag tagged KBTBD4 R313PRR (KBTBD4 Human)

Expression

Mammalian

Mutation

R313PRR

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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lenti U6-sgRNA-acRNA SYN-dCas9-P2A-EGFP

Plasmid

#159086

Purpose

Expresses dCas9-P2A-EGFP driven by human SYN promoter and empty CRISPR Display sgRNA/accessory RNA from U6 promoter.

Depositor

Jeremy Day

Article

Carullo et al Nucleic Acids Res. 2020 Aug 18. pii: 589

Insert

empty crRNA-acRNA backbone, dCas9-P2A-EGFP

Use

CRISPR and Lentiviral

Tags

EGFP and FLAG

Expression

Mammalian

Promoter

hSYN, U6

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-mTET2 CD mutant

Plasmid

#89896

Purpose

Expresses mouse TET2 CD mutant in mammalian cells

Depositor

Yue Xiong

Article

Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

Insert

TET2 (Tet2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

CD (cysteine-rich, dioxygenase domain) truncation…

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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HA-Rab8a-CA (Q67L)

Plasmid

#101050

Purpose

Expresses HA-tagged human Rab8-CA (Q67L) in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

Rab8a (RAB8A Human)

Tags

HA

Expression

Mammalian

Mutation

Q67L

Promoter

CMV

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-mGFP-Eps15DN

Plasmid

#223621

Purpose

Transient expression of human Eps15 with mGFP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

Eps15 (EPS15 Human)

Tags

mGFP

Expression

Mammalian

Mutation

Eps15 _ 95-295aa

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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Polymerase-unfused click editor - pCMV-T7-PCV2-nCas9 (LM2702)

Plasmid

#208961

Purpose

Polymerase-unfused click editor comprised of PCV2 fused to nCas9, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

PCV2-XTEN-nSpCas9-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A)

Promoter

CMV and T7

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-TRE-NS1-dTomato

Plasmid

#175278

Purpose

AAV vector mediating inducible bicistronic expression of NS1 gene of YFV-17D and dTomato

Depositor

Wei Xu

Article

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

NS1; dTomato (POLY Yellow fever virus strain 17D)

Use

AAV

Tags

dTomato (from bidirectional promoter)

Promoter

bidirectional TRE promoter

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-myr-AKT-HA

Plasmid

#31790

Depositor

Xin Chen

Article

Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

Insert

Akt1 (Akt1 Mouse)

Use

Entry vector

Tags

HA and Myr

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-HNF1A-WT-Flag-UTR

Plasmid

#183237

Purpose

Expression of FLAG tagged HNF1A

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

HNF1 homeobox A (HNF1A Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pIRIGF-IKZF1-V2

Plasmid

#69045

Purpose

expression of IKZF1 fused to firefly luciferase with co-expression of Renilla luciferase, both under IRES control.

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF1 (IKZF1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

splice variant 2

Promoter

IRES

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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