We narrowed to 14,442 results for: NTS

1. FSW-HRP-V5-LRRTM1

   Plasmid

   #82536

   Purpose

   Lentiviral vector for expressing HRP-LRRTM1 in neurons

   Depositor

   Alice Ting

   Article

   Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

   Insert

   LRRTM1 (LRRTM1 Human)
   

   Use

   Lentiviral

   Tags

   HRP and V5

   Expression

   Mammalian

   Promoter

   Human Synapsin

   Available Since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-hPPP3CC-FLAG

   Plasmid

   #179162

   Purpose

   Expression vector of human protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

   Depositor

   Masahito Ikawa

   Article

   Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

   Insert

   protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. mH2A2-CT-GFP

   Plasmid

   #45171

   Depositor

   Hunt Willard, Brian Chadwick

   Article

   Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

   Insert

   H2Afy2 (MACROH2A2, H2AFY2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   July 2, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pTwist-SARS-CoV-2 Δ18 P.1

   Plasmid

   #173476

   Purpose

   Encodes SARS-CoV-2 P.1 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 Spike Truncated to remove 18 amino acids
   

   Expression

   Mammalian

   Available Since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3_GLP1R-NTEV-TCS-GV-2xHA

   Plasmid

   #194378

   Purpose

   Split TEV assays

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   GLP1R-NTEV-TCS-GV-2xHA (GLP1R Human, Synthetic)
   

   Tags

   2xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBSFI-myr-akt-T308A/S473A

   Plasmid

   #49191

   Purpose

   cDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 and S to A at position 473 in cloning vector

   Depositor

   Peter Vogt

   Article

   Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Adaptor plasmid

   Tags

   HA tag and myristolation signal

   Mutation

   T308A; S473A

   Promoter

   T7

   Available Since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-mPpp3cc-FLAG-6XHIS

   Plasmid

   #179160

   Purpose

   Expression vector of mouse protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc) tagged with FLAG and 6X HIS at C-terminus, CAG promoter, rabbit globin poly(A) signal.

   Depositor

   Masahito Ikawa

   Article

   Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

   Insert

   protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc Mouse)
   

   Tags

   6XHIS and FLAG

   Expression

   Mammalian

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. PET-eSpCas9-plus-NLS-6xHis

   Plasmid

   #207380

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   eSpCas9-plus
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K848A, R1060A, amino acids 1005-1013 replaced wit…

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCPH2A.X_S139D-GS3

   Plasmid

   #204748

   Purpose

   Expression of Cas9 and human H2AX with the S139D mutation

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AX S139D (H2AX Human)
   

   Expression

   Mammalian

   Mutation

   H2AX with the S139D mutation

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. 2_T7-mScarlet3-KRas

    Plasmid

    #225929

    Purpose

    In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras membrane localisation signal

    Depositor

    Michalis Averof

    Article

    Karapidaki et al bioRxiv 2024.11.12.623055

    Insert

    mScarlet3-KRas
    

    Use

    In vitro transcription

    Promoter

    T7

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-MTS-WT-c-Src-FLAG

    Plasmid

    #44652

    Depositor

    Yoshimi Homma

    Article

    Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

    Insert

    v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
    

    Tags

    FLAG tag and mitochondria-targeting sequence (res…

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. HiBiT-FZD10

    Plasmid

    #195848

    Purpose

    Expresses human FZD10 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD10 (FZD10 Human)
    

    Tags

    HiBiT

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCK340

    Plasmid

    #192639

    Purpose

    Expresses dSpG and MCP-SoxS(R93A/S101A) on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Inserts

    Sp.pCas9-dSpG
    BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    SoxS has R93A and S101A mutations and dSpG has se…

    Promoter

    BBa_J23107 and Sp.pCas9

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCD565

    Plasmid

    #153027

    Purpose

    dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA

    Depositor

    Jesse Zalatan

    Article

    Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

    Insert

    dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

    Plasmid

    #139086

    Purpose

    Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCI-Neo-HKIImut

    Plasmid

    #99240

    Purpose

    Type II Hexokinase expressed in AS-30D rat hepatoma cell line

    Depositor

    Peter Pedersen

    Article

    Mathupala et al J Biol Chem. 1997 Sep 5;272(36):22776-80

    Insert

    Hexokinase II (Hk2 Rat)
    

    Expression

    Mammalian

    Mutation

    Four silent mutations; P (115)L, A (192) V, F (78…

    Promoter

    CMV I.E.

    Available Since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUPD P35S (GB0552)

    Plasmid

    #160554

    Purpose

    35s promoter for N-terminal fusions

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Nucleic Acids Res. 2017 Feb 28;45(4):219

    Insert

    35s
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCEP4-myc-HLA-B57

    Plasmid

    #135516

    Purpose

    Mammalian expression of myc-tagged HLA-B*57:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-B*57:1 (HLA-B Human)
    

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. (364) SB KRAB-SadCas9

    Plasmid

    #163022

    Purpose

    Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    Transposon

    Tags

    KRAB and myc NLS

    Expression

    Mammalian

    Promoter

    TET-On

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2-mkgDUX4

    Plasmid

    #21156

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

    Insert

    DUX4 (DUX4 Human)
    

    Expression

    Bacterial and Mammalian

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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