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We narrowed to 57,395 results for: plasmid

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Showing: 8141 - 8160 of 57395 results

  1. pRTC2-puro

    Plasmid
    #190567
    Purpose
    Expresses the full-length human L1 (L1.3) retrotransposon and an engineered neomycin retrotransposition reporter to monitor retrotransposition efficiency in cultured mammalian cells.
    Depositor
    Anthony Furano
    Article
    Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112
    Insert
    L1 (L1.3) with reporter cassette
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    190567-plasmid-map-sequence-id-393296

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  4. pSLPB2B-FKBP12_F36V-KRAB-SpdCas9-tagBFP-PGK-Blasticidin

    Plasmid
    #187957
    Purpose
    FKBP12 (F36V mutant) degron-tagged KRAB-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    FKBP12_F36V-KRAB-SpdCas9-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS
    Expression
    Mammalian
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187957-plasmid-map-sequence-id-373706

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  9. PEA1-Nuclease-Puro

    Plasmid
    #171992
    Purpose
    Delivers all prime editing nuclease components in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Expression
    Mammalian
    Mutation
    GC to CA point mutation in SpCas9 to restore nucl…
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    171992-plasmid-map-sequence-id-338139

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  10. pJRH623

    Plasmid
    #171633
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-non-targeting
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1-a and U6
    Available Since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171633-plasmid-map-sequence-id-336492

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  12. pCAT003

    Plasmid
    #171628
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-TRAC
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    CAG and EF1-a
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171628-plasmid-map-sequence-id-336485

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  14. pBJ2

    Plasmid
    #167131
    Purpose
    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter
    Depositor
    Akira Mine
    Article
    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d
    Insert
    luxCDABE
    Use
    Luciferase
    Expression
    Bacterial
    Promoter
    Kanamycin promoter
    Available Since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167131-plasmid-map-sequence-id-330437

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  15. pARC8-LambdaRedBeta-EcSSB-EcMutLE32K

    Plasmid
    #162576
    Purpose
    Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency. Contains a dominant negative mismatch repair protein.
    Depositor
    George Church
    Article
    Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038
    Inserts
    Lambda Red-Beta
    E. coli SSB
    EcMutLE32K
    Expression
    Bacterial
    Mutation
    E32K mutated to give dominant negative phenotype
    Promoter
    pBAD
    Available Since
    May 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162576-plasmid-map-sequence-id-326400

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  19. LifeAct-14 (LA-14)

    Plasmid
    #158750
    Purpose
    Lentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.
    Depositor
    Minhajuddin Sirajuddin
    Article
    Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152
    Insert
    LifeAct-14
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Deletion mutation for last three amino acids from…
    Promoter
    CMV immediate early
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158750-plasmid-map-sequence-id-310555

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Showing: 8141 - 8160 of 57395 results