We narrowed to 14,587 results for: pET

1. pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis

   Plasmid

   #176575

   Purpose

   Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

   Insert

   Dispersin B
   

   Use

   Tags

   Avitag, Hexahistidine tag, and mEGFP

   Expression

   Bacterial

   Mutation

   E184Q W330Y

   Promoter

   T7

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET29b-AviTag(K10A)-eGFP-dspB(E184Q)-6xHis

   Plasmid

   #175788

   Purpose

   Plasmid for overexpression of recombinant AviTagged(nonfunctional), His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

   Insert

   Dispersin B
   

   Use

   Tags

   AviTag, Hexahistidine tag, and eGFP

   Expression

   Bacterial

   Mutation

   E184Q

   Promoter

   T7

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET29b-AviTag-eGFP-dspB(E184Q W330Y)-6xHis

   Plasmid

   #175801

   Purpose

   Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

   Insert

   Dispersin B
   

   Use

   Tags

   AviTag, Hexahistidine tag, and eGFP

   Expression

   Bacterial

   Mutation

   E184Q W330Y

   Promoter

   T7

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a-Coh-ddFLN4-ELP(MV7E2)3-His-ybbR

   Plasmid

   #157672

   Purpose

   Expression of Coh (type III from Ruminococcus flavefaciens) with ddFLN4, ELP linker, His6x tag, and ybbR Tag.

   Depositor

   Michael Nash

   Article

   Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

   Insert

   Cohesin (R.f) with ddFLN4, ELP, Histag and ybbR tag
   

   Use

   Tags

   ddFLN4, ELP, Histag and ybbR tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-ybbR-His-ELP(MV7E2)3-ddFLN4-SpyCatcher

   Plasmid

   #157674

   Purpose

   Expression of SpyCatcher with ddFLN4, ELP linker, His6x tag, and ybbR Tag.

   Depositor

   Michael Nash

   Article

   Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

   Insert

   SpyCatcher with ddFLN4, ELP, Histag and ybbR tag
   

   Use

   Tags

   ddFLN4, ELP, Histag and ybbR tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-ybbr-XMod-Doc (BMB-KO, S199AzF)-HIS

   Plasmid

   #153445

   Purpose

   E. coli expression (amber suppression) of Rc. XDocB binding mode B knock-out mutant with serine at position 199 replaced with amber codon.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc.XDocB
   

   Use

   Tags

   6xHis and ybbr tag

   Expression

   Bacterial

   Mutation

   Serine 199 was mutated to amber codon (TAG). Argi…

   Promoter

   T7 promoter

   Available since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-ybbr-XMod-Doc (BMA-KO, S199AzF)-HIS

   Plasmid

   #153444

   Purpose

   E. coli expression (amber suppression) of Rc. XDocB binding mode A knock-out mutant with serine at position 199 replaced with amber codon.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc.XDocB
   

   Use

   Tags

   6xHis and ybbr tag

   Expression

   Bacterial

   Mutation

   Serine 199 was mutated to amber codon (TAG). Argi…

   Promoter

   T7 promoter

   Available since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET-GST-TNRC6A-(935-984)-NES-mut

   Plasmid

   #42051

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   GST and His

   Expression

   Bacterial

   Mutation

   contain aa935-984, F951A, F955A, I958A, F960A

   Promoter

   Available since

   April 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. M61 pPET28c-Xenopus beta catenin domains R10C

   Plasmid

   #17202

   Depositor

   Randall Moon

   Insert

   beta catenin (ctnnb1.L Frog)
   

   Use

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   domains R10C

   Promoter

   Available since

   Sept. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

10. M60 pPET28a-Xenopus beta catenin domains NR7

    Plasmid

    #17201

    Depositor

    Randall Moon

    Insert

    beta catenin (ctnnb1.L Frog)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    domains NR7

    Promoter

    Available since

    Sept. 12, 2012

    Availability

    Academic Institutions and Nonprofits only