162,981 results

1. TFORF0623

   Plasmid

   #142666

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   DDIT3 (DDIT3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRIM28 gRNA (BRDN0001146162)

   Plasmid

   #76142

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIM28

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIM28 (TRIM28 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNL(CMV)TK2ALuc2/CMV/WPREdU3

   Plasmid

   #163965

   Purpose

   Co-expression of HSV-1 TK and firefly luciferase in mammalian cells

   Depositor

   Jakob Reiser

   Article

   Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

   Insert

   HSV-1 TK and FLuc
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHCMV-SS-antiEEscFv-TM

   Plasmid

   #198304

   Purpose

   Displays anti-EE scFv from the surface of mammalian cells

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   pHCMV-SS-antiEEscFv-TM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSems LifeAct-StayGold

   Plasmid

   #222947

   Purpose

   Expression of LifeAct tagged with StayGold in mammalian cells

   Depositor

   Jacob Piehler

   Article

   Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:

   Insert

   LifeAct
   

   Use

   Tags

   StayGold

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. ecALDH

   Plasmid

   #67921

   Purpose

   Expresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Insert

   Escherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTol2 - myo6b:CaSR-EGFP, cryaa:mCherry

   Plasmid

   #191500

   Purpose

   GROW AT 30 DEGREES. A construct used to create transgenic fish that express CaSR-EGFP in hair cells and an mCherry marker in the lens of the eye

   Depositor

   Michael Granato

   Article

   Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

   Insert

   CaSR-EGFP-SV40
   

   Use

   Creation of transgenic fish using tol2 transgenes…

   Tags

   Expression

   Mutation

   Promoter

   myo6b

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTSara

   Plasmid

   #60720

   Purpose

   Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORF

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Inserts

   N Terminal fragment of T7 RNAP split betwen 179-180
   N Terminal fragment of T7 RNAP split betwen 179-180
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T7 RNAP split between positions 179-180 and wt T7…

   Promoter

   PBAD

   Available since

   Dec. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pN1-OPA1(123Nt)-mScarletI

   Plasmid

   #129343

   Purpose

   Mitochondrial intermembrane space localization

   Depositor

   Yasushi Okada

   Insert

   mScarlet(i) (Addgene 85044)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MC5R-Tango

    Plasmid

    #66431

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    MC5R (MC5R Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only