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pLVX-5'-aptamer-tagged-SVA-lncRNA-AK057321-mcherry

Plasmid

#202818

Purpose

Lentiviral vector to express 5'-aptamer tagged SVA-lncRNA AK057321 with CMV for mcherry expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

SVA-lncRNA AK057321

Use

Lentiviral

Expression

Mammalian

Mutation

addition of a dual aptamer RNA tag 5' to the…

Promoter

U6

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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mRFP-LAP2β

Plasmid

#228029

Purpose

lentiviral construct for mRFP-LAP2β expression

Depositor

David Pellman

Article

Zhao et al Dev Cell. 2023 May 22;58(10):847-865.e10

Insert

LAP2b (Tmpo Rat)

Use

Lentiviral

Tags

mRFP

Expression

Mammalian

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-attBr-ZsGreen-attP

Plasmid

#51265

Purpose

Bxb1-responsive fluorescent reporter plasmid containing reverse complementary ZsGreen flanked by Bxb1 attB/P recognition sites in head-to-head orientation

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

attBr-ZsGreen-attP

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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prk5-MFSD12-TEV-3xFLAG-3xHA

Plasmid

#149504

Purpose

Transient expression of human MFSD12 with recoded nucleotide sequence.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

MFSD12 (MFSD12 Human)

Use

Transient expression

Tags

TEV-3XHA-3XFLAG

Expression

Mammalian

Mutation

Codon Optimized Sequence

Promoter

CMV

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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Control click editor - pCMV-T7-PCV2-deadCas9-EcKlenow (EMK500)

Plasmid

#208944

Purpose

A control CE1 construct with catalytically inactivated SpCas9, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

PCV2-XTEN-dSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

dSpCas9(D10A/H840A);EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available Since

Nov. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-ENK

Plasmid

#184604

Purpose

For expression of a fluorescent sensor for enkephalin in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-ENK

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-TEV-HsFbxo7-129-398/Skp1

Plasmid

#202592

Purpose

Bacterial expression of HsFbxo7-129-398 and HsSkp1

Depositor

Brenda Schulman

Article

Kraus et al EMBO Rep. 2023 Aug 3;24(8):e56399. doi:

Inserts

Fbxo7 (FBXO7 Human)
Skp1 (SKP1 Human)

Tags

GST, TEV

Expression

Bacterial

Mutation

aa 129-398 only

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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LexAop2-myr::4xSNAPf

Plasmid

#87638

Purpose

For creating drosophila transgenics expressing LexAop2-myr4x::SNAPf

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

myr::4xSNAPf

Expression

Insect

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCRPVsheLL

Plasmid

#46952

Purpose

encode CRPV L1 and L2, Bicistronic, too large to self-package

Depositor

John Schiller

Article

Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

Insert

CRPV L1+L2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pTT3-ecdCD86-ST3-His

Plasmid

#210664

Purpose

Expression of the extracellular domain of human CD86 fused to Spytag003 + Histag in HEK293 (or similar)

Depositor

Omer Dushek

Article

Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

Insert

Extracellular domain of human CD86 fused to Spytag003 and Histag (CD86 Human)

Tags

Histag and Spytag003

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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