We narrowed to 3,099 results for: GAI

1. pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9

   Plasmid

   #107857

   Purpose

   To knock-out TDP43

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   gRNA against TARDBP (Exon 1) (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST- IP2.0(codon for C.elegans)

   Plasmid

   #109216

   Purpose

   IP2.0 for C. elegans (Codon optimization and introns).

   Depositor

   Takeshi Ishihara

   Article

   Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

   Insert

   IP2.0 (Inverse Pericam 2.0)
   

   Use

   Tags

   His-tag

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. ZP60 Axin2

   Plasmid

   #16882

   Depositor

   Randall Moon

   Article

   Weidinger et al Curr Biol. 2005 Mar 29. 15(6):489-500.

   Insert

   axin2 (axin2 Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3HA-ErbB4CTF

   Plasmid

   #17803

   Depositor

   Marius Sudol

   Article

   Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

   Insert

   ErbB4 (ERBB4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Carboxy-terminal fragment, amino acids 676-1292

   Promoter

   Available since

   May 9, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pXPR_BRD003 sgKEAP1-2

   Plasmid

   #74986

   Purpose

   sgKEAP1-2, puromycin selection

   Depositor

   William Hahn

   Article

   Krall et al Elife. 2017 Feb 1;6:e18970. doi: 10.7554

   Insert

   KEAP1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSCALPS-Flag-Mili-EGFP

   Plasmid

   #212576

   Purpose

   Lentiviral expression of Flag-tagged mouse Mili in mammalian cells

   Depositor

   Phillip Zamore

   Article

   Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

   Insert

   Mili (Piwil2 Mouse)
   

   Use

   Lentiviral

   Tags

   5x Flag and SNAP tag

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTorPE-GEM-GECO1

   Plasmid

   #32463

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   GEM-GECO1
   

   Use

   Lab constructed

   Tags

   6x His and modified TorA MNNNDLFQASRRRFLAQLG[G to…

   Expression

   Bacterial

   Mutation

   GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

   Promoter

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCold I-Hero7

   Plasmid

   #187926

   Purpose

   Bacterial expression of heat-resistant obscure (Hero) protein Hero7

   Depositor

   Yukihide Tomari

   Article

   Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

   Insert

   Hero7 (SERF2 Human)
   

   Use

   Tags

   Flag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCold I-Hero45

   Plasmid

   #187931

   Purpose

   Bacterial expression of heat-resistant obscure (Hero) protein Hero45

   Depositor

   Yukihide Tomari

   Article

   Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

   Insert

   Hero45 (SERBP1 Human)
   

   Use

   Tags

   Flag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMKO.1 puro-luc (shRNA)

    Plasmid

    #153962

    Purpose

    Expresses a shRNA against firefly luciferase

    Depositor

    Hiroyuki Konishi

    Article

    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

    Insert

    shRNA against firefly luciferase (19-bp-long target)
    

    Use

    Luciferase, RNAi, and Retroviral

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEJS1026-pMCSG7-HpaCas9

    Plasmid

    #121540

    Purpose

    Expresses a 6X-His tagged type II-C Cas9 from H. parainfluenzae in bacterial cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    HpaCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEJS1027-pMCSG7-SmuCas9

    Plasmid

    #121541

    Purpose

    Expresses a 6X-His tagged type II-C Cas9 from S. muelleri in bacterial cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    SmuCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSW554 - pOp5:SP-mVenus-HDEL

    Plasmid

    #116006

    Purpose

    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

    Depositor

    Sebastien Wolf

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    pOp5:SP(ER)-mVenus-HDEL:tUBQ10::SulfR
    

    Use

    Tags

    HDEL and SP(ER)

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pM-ErbB4CTF

    Plasmid

    #17797

    Depositor

    Marius Sudol

    Article

    Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

    Insert

    ErbB4 (ERBB4 Human)
    

    Use

    Tags

    GAL4-BD

    Expression

    Mammalian

    Mutation

    Carboxy-terminal fragment only, amino acids 676-1…

    Promoter

    Available since

    May 9, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pT2-7xTcf-NLS-ONL

    Plasmid

    #65713

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, ONL
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. Myc-Foxo1-WT 6KR

    Plasmid

    #17558

    Depositor

    Domenico Accili

    Article

    Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

    Insert

    Foxo1 6KR (Foxo1 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K242R, K246R, K259R, K262R, K271R, K291R

    Promoter

    CMV

    Available since

    Oct. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9

    Plasmid

    #107858

    Purpose

    To knock-out TDP43

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    gRNA against TARDBP (Exon 2) (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. sgFFL

    Plasmid

    #59877

    Purpose

    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene.

    Depositor

    Georg Seelig

    Article

    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.

    Insert

    mCherry with intron containing the mouse mir-124–3
    

    Use

    Tags

    PEST and VAMP 3 UTR

    Expression

    Mammalian

    Mutation

    Promoter

    doxycycline (Dox)-inducible promoter (CMV/TO)

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. PHR-GFP

    Plasmid

    #183926

    Purpose

    Optogenetic PHR domain coupled to GFP; binds to CIBN upon blue light exposure

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    PHR-GFP (CRY2 Mustard Weed, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. psiCHECK CHAC1 3'UTR

    Plasmid

    #19861

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    CHAC1 3'UTR (CHAC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge