We narrowed to 1,590 results for: PTS

1. pHR-YTHDC1-FL-mCherry-Cry2

   Plasmid

   #177124

   Purpose

   Express Full-length YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay

   Depositor

   Wenbo Li

   Article

   Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

   Insert

   YTHDC1 (YTHDC1 Human)
   

   Use

   Tags

   mCherry-CRY2

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. plenti-ef1a-dCasRx-FTO-HA-T2A-BSD

   Plasmid

   #177120

   Purpose

   dCasRx mediated RNA tethering of FTO

   Depositor

   Wenbo Li

   Article

   Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

   Insert

   dCasRx-FTO
   

   Use

   Lentiviral

   Tags

   HA and SV40 NLS

   Expression

   Mutation

   dCasRX contains R239A/H244A/R858A/H863A mutations…

   Promoter

   Ef1a

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHR-IDR1-mCherry-Cry2

   Plasmid

   #177125

   Purpose

   Express IDR1 fragment of YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay

   Depositor

   Wenbo Li

   Article

   Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

   Insert

   YTHDC1 (IDR1 domain) (YTHDC1 Human)
   

   Use

   Tags

   mCherry-CRY2

   Expression

   Mammalian

   Mutation

   IDR1 domain only

   Promoter

   SFFV

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRSF_his6_sumo_ZIKV_NS5_MTase

   Plasmid

   #213529

   Purpose

   Expresses the MTase domain of NS5 of ZIKV

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   ZIKA virus NS5 methyltransferase domain
   

   Use

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   MTase domain aa 1-265

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1_ZKSCAN1_Tau10-12K317M

   Plasmid

   #194164

   Purpose

   Insert contains intronic ZKSCAN1 Alu repeat elements flanked by tau cDNA exons 10-12 with FTLD-Tau K317M mutation. Expresses the tau circRNA 12-->10 K317M with 3X flag tag in exon 10.

   Depositor

   Stefan Stamm

   Article

   Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

   Insert

   Microtubule-associated protein tau 10-12 WT (MAPT Human)
   

   Use

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Changed Lysine 317 to Methionine

   Promoter

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHR-YTH-mCherry-Cry2

   Plasmid

   #177126

   Purpose

   Express YTH domain fragment of YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay

   Depositor

   Wenbo Li

   Article

   Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

   Insert

   YTHDC1 (YTH domain) (YTHDC1 Human)
   

   Use

   Tags

   mCherry-CRY2

   Expression

   Mammalian

   Mutation

   YTH domain only

   Promoter

   SFFV

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HI.HslU.RSETB.fl.wt

   Plasmid

   #12556

   Depositor

   David McKay

   Article

   Trame et al Acta Crystallogr D Biol Crystallogr. 200

   Insert

   Haemophilus influenzae heat shock locus U
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-Ef1a-mClover-W377A/W428A YTHDC1-T2A-BSD-siRNA-Resistant

   Plasmid

   #177130

   Purpose

   Generate lentivirus to introduce mClover tagged m6A binding null mutant W377A/W428A YTHDC1

   Depositor

   Wenbo Li

   Article

   Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

   Insert

   YTHDC1 (YTHDC1 Human)
   

   Use

   Lentiviral

   Tags

   mClover3

   Expression

   Mutation

   W428A/W377A (m6A binding defects), and siRNA resi…

   Promoter

   Ef1a

   Available since

   July 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRSF_his6_sumo_hRNMT_RAM

   Plasmid

   #213528

   Purpose

   Expresses the human RNMT-RAM complex

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Inserts

   human RNMT (RNMT Human)
   RAM
   

   Use

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRSF_his6_sumo_SARS_CoV2_NSP14

    Plasmid

    #213518

    Purpose

    Expresses NSP14 of SARS-CoV-2

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    N-terminal His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSF_his6_sumo_OC43_NSP14

    Plasmid

    #213519

    Purpose

    Expresses NSP14 of hCoV OC43

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    hCoV OC43 NSP14 (ORF1ab hCoV OC43)
    

    Use

    Tags

    His-SUMO tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRSF_his6_sumo_NL63_NSP14

    Plasmid

    #213520

    Purpose

    Expresses NSP14 of hCoV NL63

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    hCoV NL63 NSP14 (ORF1ab hCoV NL63)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRSF_his6_sumo_229E_NSP14

    Plasmid

    #213521

    Purpose

    Expresses NSP14 of hCoV 229E

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    hCoV 229E NSP14
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTXBI-mClover-YTH domain

    Plasmid

    #177123

    Purpose

    Bacterial expression of YTH domain of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification

    Depositor

    Wenbo Li

    Article

    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

    Insert

    YTHDC1 (YTH domain) (YTHDC1 Human)
    

    Use

    Tags

    Mxe GyrA intein-Chitin binding domain for protein…

    Expression

    Bacterial

    Mutation

    YTH domain only (IDR1 and IDR2 deletion)

    Promoter

    T7

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTXBI-mclover-YTHDC1-FL

    Plasmid

    #177121

    Purpose

    Bacterial expression of Full-length YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification

    Depositor

    Wenbo Li

    Article

    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

    Insert

    YTHDC1 (YTHDC1 Human)
    

    Use

    Tags

    Mxe GyrA intein-Chitin binding domain for protein…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTXBI-mclover-YTH-IDR2

    Plasmid

    #177122

    Purpose

    Bacterial expression of YTH-IDR2 fragment of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification

    Depositor

    Wenbo Li

    Article

    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

    Insert

    YTHDC1 (YTH and IDR2 domains) (YTHDC1 Human)
    

    Use

    Tags

    Mxe GyrA intein-Chitin binding domain for protein…

    Expression

    Bacterial

    Mutation

    YTH and IDR2 domains only (IDR1 deletion)

    Promoter

    T7

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pShuttle-FEN1mED

    Plasmid

    #35033

    Depositor

    Sheila Stewart

    Article

    Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

    Insert

    FEN1mWT
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    PT MUTANT E160D

    Promoter

    CMV

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pffDronpa2F-peroxisome

    Plasmid

    #214608

    Purpose

    Expresses reversibly switchable fluorescent protein ffDronpa2F targeted to peroxisome

    Depositor

    Peter Dedecker

    Article

    Valenta et al Talanta. 2024 Mar

    Insert

    ffDronpa2F-peroxisome
    

    Use

    Tags

    peroxisome targeting sequence (PTS)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSKB3.EG10680

    Plasmid

    #11436

    Depositor

    Sung-Hou Kim

    Article

    Shin et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    OsmC (osmC E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 10, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pRSF_his6_sumo_SARS_CoV2_NSP14_D90A/E92A

    Plasmid

    #213527

    Purpose

    Expresses NSP14 of SARS-CoV-2 with two point mutations D90A and E92A

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    NSP14 of SARS-CoV-2 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    D90A and E92A

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge