2,159 results

1. pCRII-TOPO-dgat1a in situ probe

   Plasmid

   #232137

   Purpose

   zebrafish dgat1a anti-sense in situ probe, linearize with NotI, SP6 polymerase

   Depositor

   Steven Farber

   Article

   Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

   Insert

   dgat1a (dgat1a Zebrafish)
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   SP6/T7

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCRII-TOPO-dgat1b in situ probe

   Plasmid

   #232138

   Purpose

   zebrafish dgat1b anti-sense in situ probe, linearize with NotI, SP6 polymerase

   Depositor

   Steven Farber

   Article

   Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

   Insert

   dgat1b
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   SP6/T7

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCRII-TOPO-mogat2 in situ probe

   Plasmid

   #232139

   Purpose

   zebrafish mogat2 anti-sense in situ probe, linearize with NotI, SP6 polymerase

   Depositor

   Steven Farber

   Article

   Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

   Insert

   mogat2 (mogat2 Zebrafish)
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   SP6/T7

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCRII-dgat2 in situ probe

   Plasmid

   #232134

   Purpose

   zebrafish dgat2 anti-sense in situ probe, linearize with BamHI, T7 polymerase

   Depositor

   Steven Farber

   Article

   Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

   Insert

   dgat2 (dgat2 Zebrafish)
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   SP6/T7

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLL5.0_shRNAHsCavin1_Del4UC1_EGFP

   Plasmid

   #229691

   Purpose

   Lentiviral expression of shRNA targeting Cavin1 + reconstitution of zebrafish mutant Cavin1 (Del4UC1)

   Depositor

   Alpha Yap

   Article

   Brooks et al Mol Biol Cell. 2023 Nov 1;34(12):ar120.

   Inserts

   shRNA for hCavin1 (CAVIN1 Human)
   mutant cavin1b (Del4UC1) (cavin1b Zebrafish)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Del4UC1

   Promoter

   LTR viral promoter

   Available since

   Jan. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLL5.0_shRNAHsCavin1_DrCavin1b_EGFP

   Plasmid

   #229690

   Purpose

   Lentiviral expression of shRNA targeting Cavin1 + reconstitution of zebrafish Cavin1 (Cavin1 rescue)

   Depositor

   Alpha Yap

   Article

   Brooks et al Mol Biol Cell. 2023 Nov 1;34(12):ar120.

   Inserts

   shRNA for hCavin1 (CAVIN1 Human)
   cavin1b (cavin1b Zebrafish)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   LTR viral promoter

   Available since

   Jan. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCS2TAL3-RR-zrif1_exon8

   Plasmid

   #194434

   Purpose

   Right (RR) TALEN for mutating zebrafish rif1 gene

   Depositor

   Christopher Sansam

   Article

   Masser et al eLife 2023 (Reviewed Preprint) 12:RP8767

   Insert

   zrif1_ex8_RIGHT (rif1 Zebrafish)
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   CMV IE94

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCS2TAL3-DD-zrif1_exon8

   Plasmid

   #194435

   Purpose

   Left (DD) TALEN for mutating zebrafish rif1 gene

   Depositor

   Christopher Sansam

   Article

   Masser et al eLife 2023 (Reviewed Preprint) 12:RP8767

   Insert

   zrif1_ex8_LEFT (rif1 Zebrafish)
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   CMV IE94

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUC57-GATA1

   Plasmid

   #194436

   Purpose

   In Situ hybridization probes

   Depositor

   Christopher Sansam

   Article

   Masser et al eLife 2023 (Reviewed Preprint) 12:RP8767

   Insert

   GATA1a (gata1a Zebrafish)
   

   Use

   In situ hybridization

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC57 β-Globin

    Plasmid

    #194437

    Purpose

    In Situ hybridization probes

    Depositor

    Christopher Sansam

    Article

    Masser et al eLife 2023 (Reviewed Preprint) 12:RP8767

    Insert

    β-globin
    

    Use

    In situ hybridization

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCS2+-Zeo-miR-430-G-G mm

    Plasmid

    #221728

    Purpose

    Expression of Zeocin resistance marker followed by miR-430 target site insertion with G-G mismatch at position 6 in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    BleoR-miR-430-G-G mm
    

    Use

    Fish expression

    Tags

    Expression

    Mutation

    G-G mismatch of miR-430 site

    Promoter

    simian CMV IE94

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCS2+-Zeo-miR-430-10-11 mm

    Plasmid

    #221727

    Purpose

    Expression of Zeocin resistance marker followed by miR-430 target site insertion with mismatches at position 10 and 11 in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    BleoR-miR-430-10-11 mm
    

    Use

    Fish expression

    Tags

    Expression

    Mutation

    Mismatch at positions 10 and 11 of miR-430 site

    Promoter

    simian CMV IE94

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCS2+-Zeo-miR-430

    Plasmid

    #221726

    Purpose

    Expression of Zeocin resistance marker followed by perfect miR-430 target site insertion in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    BleoR-miR-430
    

    Use

    Fish expression

    Tags

    Expression

    Mutation

    Promoter

    simian CMV IE94

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCS2+-FLAG3-hsN-drAgo2

    Plasmid

    #221717

    Purpose

    Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for human Ago2 N-domain.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    hsN-drAgo2 (ago2 Zebrafish)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    Swap of N-domain for human Ago2 N-domain

    Promoter

    simian CMV IE94

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCS2+-FLAG3-drAgo2D-A

    Plasmid

    #221713

    Purpose

    Expression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2D-A (ago2 Zebrafish)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    D683A in active site

    Promoter

    simian CMV IE94

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p104Tol2-tp1:Cas9-t2A-GFP, 4xU6:sgRNA

    Plasmid

    #227773

    Purpose

    Expression of tp1 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs in zebrafish

    Depositor

    Bushra Raj

    Article

    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

    Inserts

    Cas9-t2A-GFP
    4xU6:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6 and tp1

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p155ME-loxP-dsR-loxP-Cas9-t2A-GFP

    Plasmid

    #227774

    Purpose

    Middle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloning

    Depositor

    Bushra Raj

    Article

    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

    Insert

    loxP-dsR-loxP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p103DestTol2pA2-4xU6sgRNA

    Plasmid

    #227778

    Purpose

    Destination vector with U6 driven 4 sgRNAs

    Depositor

    Bushra Raj

    Article

    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

    Insert

    4xU6:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p128Tol2-Olactb:Cas9-t2A-GFP, 4xU6:sgRNA

    Plasmid

    #226834

    Purpose

    Expression of b-actin driven Cas9-GFP and U6-driven 4 sgRNAs in zebrafish

    Depositor

    Bushra Raj

    Article

    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

    Inserts

    Cas9-t2A-GFP
    4xU6:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Olactb and U6

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTol1 - 14xUAS:CaSR-EGFP

    Plasmid

    #191502

    Purpose

    A UAS construct used to create transgenic fish that express CaSR-EGFP when crossed to a fish carrying a Gal4.

    Depositor

    Michael Granato

    Article

    Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

    Insert

    CaSR-EGFP-SV40
    

    Use

    Creation of transgenic fish using tol1 transgenes…

    Tags

    Expression

    Mutation

    Promoter

    14X UAS

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCS2-hcar1-4-mKate2

    Plasmid

    #226712

    Purpose

    overexpression red fluorescent hcar1-4 protein in zebrafish larvae

    Depositor

    Philipp Niethammer

    Article

    Ma et al Cell Rep. 2023 Jan 31;42(1):111974. doi:

    Insert

    hcar1-4
    

    Use

    Unspecified; Zebrafish expression

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGEX-6P--Dr-aCateninFL

    Plasmid

    #191740

    Purpose

    Expresses FL D rerio catenin in bacteria

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

    Insert

    CTNNA1 (ctnna1 Zebrafish)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDestTol2pA2-5.0dand5:EGFP

    Plasmid

    #223327

    Purpose

    Expresses EGFP in Kupffer's vesicle cells in zebrafish embryos

    Depositor

    Hiroyuki Takeda

    Article

    Ikeda et al Dev Growth Differ. 2022 Apr;64(3):163-17

    Insert

    5.0kb dand5 promoter (dand5 Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-FLAG3-drAgo2D-A

    Plasmid

    #221734

    Purpose

    Expression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in mammalian cells.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2D-A (ago2 Zebrafish)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D683A in active site

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3-FLAG3-drAgo2DY-EF

    Plasmid

    #221733

    Purpose

    Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in mammalian cells.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2DY-EF (ago2 Zebrafish)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D651E and Y680F in active site

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-FLAG3-drAgo2Y-F

    Plasmid

    #221732

    Purpose

    Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in mammalian cells.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2Y-F (ago2 Zebrafish)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Y680F in active site

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-FLAG3-drAgo2D-E

    Plasmid

    #221731

    Purpose

    Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in mammalian cells.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2D-E (ago2 Zebrafish)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D651E in active site

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-FLAG3-drAgo2

    Plasmid

    #221730

    Purpose

    Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in mammalian cells.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2 (ago2 Zebrafish)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCS2+-FLAG3-drAgo2Y-F

    Plasmid

    #221715

    Purpose

    Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2Y-F (ago2 Zebrafish)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    Y680F in active site

    Promoter

    simian CMV IE94

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCS2+-FLAG3-drAgo2D-E

    Plasmid

    #221714

    Purpose

    Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    drAgo2D-E (ago2 Zebrafish)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    D651E in active site

    Promoter

    simian CMV IE94

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge