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pEM705 Gsi (Ser)

Plasmid

#109352

Purpose

GsX chimera with Gαi C-terminus (Pertussis toxin insensitive mutant)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gsi chimera

Tags

None

Expression

Mammalian

Mutation

Changed Cys at position 391 to Serine (Pertussis …

Promoter

CAG

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Blast NT shRNA

Plasmid

#123306

Purpose

Expression of a non-targeting shRNA

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

Non-targeting shRNA

Use

Lentiviral and RNAi

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_EYFP

Plasmid

#167858

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCMU-PMr

Plasmid

#61180

Purpose

Fluorescent reporter for plasma membrane AtPIP2a-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtPIP2a

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pSb_init containing the MBP sybody Sb_MBP#1

Plasmid

#132699

Purpose

expresses the MBP sybody Sb_MBP#1 in E.coli, to use as positive control in ELISA

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

sybody_MBP#1

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pShuttle-FEN1hWT

Plasmid

#35027

Depositor

Sheila Stewart

Article

Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

Insert

FEN1 (FEN1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
EcSTH

Plasmid

#211923

Purpose

This plasmid contains human codon optimized sequence of EcSTH which can be used to increase NADH/NAD ratio and decrease NADPH/NADP ratio in mammalian cells.

Depositor

Binghui Li

Article

Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

Insert

EcSTH

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Blasto

Plasmid

#167904

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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RUBY-ABE#2

Plasmid

#232176

Purpose

T-DNA vector with ecTadA8e-zCas9D10A and corresponding sgRNA to correct RUBYm2 and recover a vivid red color visible to the naked eyes.

Depositor

Yiping Qi

Article

Sexton et al Plant Physiol. 2025 Jul 3;198(3):kiaf265

Insert

2x35s-ecTadA8e-SpCas9-D10A-AtU3-sgRNA-mis4-gRNA scaffold

Use

CRISPR

Tags

3xflag and NLS

Expression

Plant

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-Jmjd3-F

Plasmid

#17440

Purpose

Expression of Jmjd3 in mammalian cells

Depositor

Kai Ge

Article

Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

Insert

JMJD3 (Kdm6b Mouse)

Tags

6xMyc and Flag

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

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Kras-Src FRET biosensor

Plasmid

#78302

Purpose

FRET biosensor. To monitor Src kinase activity in mammalian cells by FRET

Depositor

Yingxiao Wang

Article

Ouyang et al Proc Natl Acad Sci U S A. 2008 Sep 23;10

Insert

Src biosensor

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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hSERT-N1-pEYFP

Plasmid

#70105

Purpose

mammalian expression of hSERT with C-terminal YFP fusion

Depositor

Harald Sitte

Article

Just et al J Biol Chem. 2004 Feb 20;279(8):6650-7.

Insert

SERT (SLC6A4 Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-H2B-mScarlet-I

Plasmid

#182297

Purpose

Localization of mScarlet-I fluorescent protein to histone 2B

Depositor

Severine Degrelle, Thierry Fournier

Article

Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

Insert

mScarlet-I

Tags

mScarlet-I

Expression

Mammalian

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lse_C2c2_WT

Plasmid

#83486

Purpose

Bacterial expression of WT L. seeligeri C2c2 CRISPR effector

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lse_C2c2_WT

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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YF-Rac1(CA)

Plasmid

#20150

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP-Rac1(CA) (RAC1 Human)

Expression

Mammalian

Mutation

constitutively active mutation

Available Since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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pMpGE013

Plasmid

#108681

Purpose

All-in-one genome editing vector in M. polymorpha (hygromycin resistant)

Depositor

Takayuki Kohchi, Shigeo Sugano

Article

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc3-NEDD8

Plasmid

#19943

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

NEDD 8 (Neural precursor cell Expressed, Developmentally Down-regulated 8 (NEDD8 Human)

Tags

myc3

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
p5E-EF1a/b-actin

Plasmid

#82583

Purpose

5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expression

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

EF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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hSERT-N1-pECFP

Plasmid

#70106

Purpose

mammalian expression of hSERT with C-terminal CFP fusion

Depositor

Harald Sitte

Article

Just et al J Biol Chem. 2004 Feb 20;279(8):6650-7.

Insert

SERT (SLC6A4 Human)

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
p3E-FRT-KanR-FRT pA

Plasmid

#82601

Purpose

3' entry vector containing FRT-flanked kanamycin resistance cassette (KanR) with pA for FLP-induced kanamycin resistance, drug selection

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRT-Kan-FRT

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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