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We narrowed to 14,449 results for: eng

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Showing: 8181 - 8200 of 14449 results

  4. pMP650

    Plasmid
    #135000
    Purpose
    VP64-m6A Reader. Activates transcription near Gm6ATC sites. pUBC-DpnI(aa146-254)-VP64-V5 pGK-ZeoR
    Depositor
    Ahmad Khalil
    Publication
    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.
    Insert
    pUBC-DpnI(aa146-254)-VP64-V5 pGK-ZeoR
    Use
    Lentiviral and Synthetic Biology
    Tags
    DpnI(aa146-254), V5 tag, and VP-64
    Expression
    Mammalian
    Mutation
    DpnI truncated to aa146-254
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    135000-plasmid-map-sequence-id-267928

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  5. pNOC_hfnCas12a_Nlux_(-HH)crRNA NR1

    Plasmid
    #176250
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1 without the hammerhead ribozym
    Depositor
    John van der Oost
    Publication
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized fnCas12a
    Use
    CRISPR and Synthetic Biology; Expression in micro…
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176250-plasmid-map-sequence-id-346579

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  6. pCJ203

    Plasmid
    #162678
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaF2-A75; codon optimized for expression in E.…
    Promoter
    T7/lac
    Available since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162678-plasmid-map-sequence-id-321524

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  10. S1 1029 CP ABEMax strategy 1

    Plasmid
    #135357
    Purpose
    Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the N-terminus
    Depositor
    Alex Hewitt
    Publication
    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ABEMax-nSpyCas9 aa 1029
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    WT
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135357-plasmid-map-sequence-id-267707

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  11. 1029-AIDx-CP

    Plasmid
    #135341
    Purpose
    Expression of circular permutant nSpyCas9 at amino acid position 1029 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.
    Depositor
    Alex Hewitt
    Publication
    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CP 1029 nSpyCas9-hAIDx
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    WT
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135341-plasmid-map-sequence-id-267692

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  13. PA-ecliptic pHluorin

    Plasmid
    #137837
    Purpose
    A photoactivatable GEVI variant originated from Bongwoori-R3
    Depositor
    Bradley Baker
    Publication
    Lee et al Front Cell Neurosci. 2019 Oct 29;13:482.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Photoactivatable-ecliptic pHluorin
    Expression
    Mammalian
    Mutation
    L64F, T65S, V163A, T203H
    Promoter
    CMV
    Available since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    137837-plasmid-map-sequence-id-274632

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  15. pRP_8xgRNA-FLuc

    Plasmid
    #135937
    Purpose
    Firefly luciferase reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation.
    Depositor
    Antonio Amelio
    Publication
    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    minCMV
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    135937-plasmid-map-sequence-id-271493

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Showing: 8181 - 8200 of 14449 results