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POT7-RFP

Plasmid

#65323

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe-puro-TGFβRII-HA

Plasmid

#37040

Depositor

Sam Thiagalingam

Publication

Papageorgis et al Cancer Res. 2011 Feb 1;71(3):998-1008. E

Insert

TGFβRII

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

5'LTR

Available since

Jan. 10, 2013

Availability

Academic Institutions and Nonprofits only
pCR8-DJ1-M26I

Plasmid

#29357

Depositor

Mark Cookson

Publication

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Use

Gateway Cloning

Mutation

Met26Ile

Available since

March 31, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-RPS6KL1

Plasmid

#23842

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RPS6KL1 (RPS6KL1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-TPK1

Plasmid

#23659

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TPK1 (TPK1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-ADCK4

Plasmid

#23711

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ADCK4 (ADCK4 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CSNK1A1L

Plasmid

#23784

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CSNK1A1L (CSNK1A1L Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-MGC42105

Plasmid

#23596

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MGC42105 (NIM1 Human)

Use

Gateway Cloning

Mutation

Y5F

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PHKA1

Plasmid

#23471

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PHKA1 (PHKA1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pAIP53

Plasmid

#258140

Purpose

Cloning vector with an N-terminal fusion of 10x His-tagged SUMO-1, followed by a Golden Gate assembly site for seamless insertion. No cloning scars

Depositor

Christopher Bahl

Publication

Cárcamo Noriega et al Protein Sci. 2026 Sep;35(9):e70746. doi:

Insert

Smt3 (SMT3 )

Tags

HisTag (N terminal on insert)

Expression

Bacterial

Promoter

T7

Available since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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pAVEC0_N-mEGFP

Plasmid

#231255

Purpose

For expressing fusion proteins with N terminally tagged mEGFP in plants

Depositor

Jacob Brunkard

Publication

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mEGFP

Expression

Plant

Mutation

N/A

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_N-mTurquoise2

Plasmid

#231286

Purpose

For transiently expressing fusion proteins with N terminally tagged mTurquoise2 in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Publication

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mTurquoise2

Expression

Plant

Mutation

N/A

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC2_C-mTurquoise2

Plasmid

#231294

Purpose

For transiently expressing fusion proteins with C terminally tagged mTurquoise2 in plants (includes p19 silencing suppressor)

Depositor

Jacob Brunkard

Publication

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mTurquoise2

Expression

Plant

Mutation

N/A

Available since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCGFP-BC

Plasmid

#161959

Purpose

Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).

Depositor

Eric Gouaux

Publication

Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1

Type

Empty backbone

Tags

GFPuv His8

Expression

Bacterial

Promoter

T7

Available since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLCS110

Plasmid

#209331

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mStayGold fusion protein.

Depositor

Lucia Strader

Publication

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRC3EGFP

Plasmid

#193790

Purpose

Expresses EGFP under the control of PR promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Publication

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

EGFP

Expression

Bacterial

Promoter

PR promoter

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pICE HIS3

Plasmid

#122877

Purpose

HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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Syt2

Plasmid

#186688

Purpose

Expresses Synaptotagmin 2 in E. Coli

Depositor

Axel Brunger

Publication

Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

Insert

Synaptotagmin 2

Tags

GST and thrombin site

Expression

Bacterial

Promoter

T7

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSB_init_Sb68

Plasmid

#159423

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb68

Depositor

Dianfan Li

Publication

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb68

Tags

c-Myc and poly-His

Expression

Bacterial

Available since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pJ2042.2_invRep

Plasmid

#140688

Purpose

3-color stripe (inverted reporters)

Depositor

Yolanda Schaerli

Publication

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

3-color stripe (inverted reporters)

Use

Synthetic Biology

Expression

Bacterial

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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