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GEARBOCS-Vamp2-Tag-HA

Plasmid

#218188

Purpose

To tag endogenous mouse VAMP2 with HA

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Vamp2 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAF146

Plasmid

#222377

Purpose

Express Tasmanian devil PDL1 protein (saha_PDL1_FL_DN11026_c0_g1_i1)

Depositor

Andrew S. Flies

Article

Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

Insert

PDL1

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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Vector 1174K

Plasmid

#221017

Purpose

Fluorescent based sex separator in Anopheles species mosquitoes (SEPARATOR)

Depositor

Omar Akbari

Article

Weng et al bioRxiv [Preprint]. 2024 Jul 18:2024.07.

Insert

Engineered dobulesex splicing module (Anopheles gambiae)

Use

Synthetic Biology

Expression

Insect

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pSL1360

Plasmid

#219777

Purpose

pDEF-TurboID-GFPmut2, empty vector for creating C-terminal fusion proteins.

Depositor

Scott Lindner

Article

Rios et al bioRxiv [Preprint]. 2024 Mar 16:2024.02.

Insert

TurboID/GFPmut2

Use

Plasmodium, rodent malaria parasites

Promoter

None

Available Since

Aug. 2, 2024

Availability

Academic Institutions and Nonprofits only

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CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

Plasmid

#222987

Purpose

Bacterial expression of iGeoCas9(C1) construct

Depositor

Jennifer Doudna

Article

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

Use

CRISPR

Tags

CL7 and His

Expression

Bacterial

Promoter

tac

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18-LucFF

Plasmid

#220496

Purpose

To generate substrates to monitor Ku overloading and its impact on transcription at the DNA ends vicinity.

Depositor

Sébastien Britton

Article

Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

Insert

Firefly Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/T0-eGFP NFATC2IP

Plasmid

#221433

Purpose

Mammalian expression vector of eGFP tagged NFATC2IP

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Tags

eGFP

Expression

Mammalian

Mutation

1st Methionine of NFATC2IP deleted

Promoter

CMV

Available Since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/T0-3xFLAG-NFATC2IP

Plasmid

#221208

Purpose

Mammalian expression vector of triple Flag tagged NFATC2IP

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Tags

3XFlag

Expression

Mammalian

Mutation

1st Methionine deleted

Promoter

CMV

Available Since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLX-EF1a_NKX2-1_W208L

Plasmid

#221486

Purpose

NKX2-1 W208L DNA binding mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

W208L

Promoter

EF1a

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_W208L

Plasmid

#221474

Purpose

Gateway cloning of NKX2-1 W208L DNA binding mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway Cloning

Mutation

W208L

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_V205F

Plasmid

#221473

Purpose

Gateway cloning of NKX2-1 V205F DNA binding mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway Cloning

Mutation

V205F

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_DelNTerm_160-371AA

Plasmid

#221475

Purpose

Gateway cloning of NKX2-1 N-terminal truncation mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway Cloning

Mutation

Deletion of 1-159AA N-terminal domain

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_V205F

Plasmid

#221485

Purpose

NKX2-1 V205F DNA binding mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

V205F

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-SFFV_NKX2-1

Plasmid

#221496

Purpose

NKX2-1 short isoform lentiviral overexpression using SFFV promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

SFFV

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pSL1419

Plasmid

#219778

Purpose

pDEF-TurboID-GFPmut2, negative control for cytosolic expression

Depositor

Scott Lindner

Article

Rios et al bioRxiv [Preprint]. 2024 Mar 16:2024.02.

Insert

TurboID/GFPmut2

Use

Plasmodium, rodent malaria parasites

Promoter

PbEF1alpha promoter

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-LOVPLD-mCherry-OSBP(PH)

Plasmid

#219561

Purpose

Golgi-targeted LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

LOVPLD-mCherry-OSBP(PH)

Tags

mCherry

Expression

Mammalian

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB11785

Plasmid

#212699

Purpose

gRNA targeting to the intergradtion site E4

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E4

Expression

Yeast

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB10769

Plasmid

#212694

Purpose

gRNA targeting to the intergradtion site E2

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E2

Expression

Yeast

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB12719

Plasmid

#212702

Purpose

gRNA targeting to the intergradtion site F3

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site F3

Expression

Yeast

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB12720

Plasmid

#212704

Purpose

gRNA targeting to the gene 4HPPD locus

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the gene 4HPPD locus

Expression

Yeast

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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