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Addgene

We narrowed to 22,800 results for: promoter

Showing: 8201 - 8220 of 22800 results
  1. PLVX_SPTA1_IGF2BP1_IRES_PURO

    Plasmid
    #100016
    Purpose
    To over-express IGF2BP1 in Adult Erythroblasts using tissue specific promoter with IRES for puromycin selection.
    Depositor
    Insert
    Insulin like growth factor 2 mRNA binding protein 1 (IGF2BP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SPTA1 erythroid specific promoter
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. LacI-pLacI-pIII pm

    Plasmid
    #218100
    Purpose
    Phagemid that expresses LacI, and pIII under the Lac promoter
    Depositor
    Insert
    pIII
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Lac promoter
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. miABE

    Plasmid
    #205412
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Insert
    pU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D61A+E85R+H369R+S387R+S457R
    Promoter
    hU6, CAG, CMV
    Available since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pLX_TRC311/NLS-Cas13d-P2A-Blast

    Plasmid
    #212196
    Purpose
    CasRx (NLS-RfxCas13d-NLS) with 2A-Blasticidin for RNA targeting. CasRx-2A-Blasticidin is driven by EF1a promoter. EGFP is driven by SV40
    Depositor
    Insert
    CasRx (NLS-RfxCas13d-NLS)-HA with 2A-Blasticidin
    Use
    CRISPR and Lentiviral
    Tags
    HA, NLS, and blasticidin S deaminase
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pCAGGS-PHFF-sh-hBVRA

    Plasmid
    #100285
    Purpose
    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cells
    Depositor
    Inserts
    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for human BVRA
    Use
    Tags
    FLAG, T7 and Mitochondria-targeting sequence (MTS…
    Expression
    Mammalian
    Mutation
    All genes are codon-optimized for expression in h…
    Promoter
    CAG promoter and H1 promoter
    Available since
    Sept. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. pCBh_3x Flag-NLS-enRhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197027
    Purpose
    Vector encoding a human codon-optimized enRhCas12f1 driven by CBh promoter, guide RNAs compatible with enRhCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Insert
    humanized enRhCas12f1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L270R
    Promoter
    CBh, CMV, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. miniCRISPRoff-v1

    Plasmid
    #197030
    Purpose
    Vector encoding a human codon-optimized miniCRISPRoff-v1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Insert
    humanized miniCRISRPoff-v1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D52R / T132R / D228A / D406A
    Promoter
    CBh, CMV, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. gGBEv6.3-SpCas9

    Plasmid
    #202629
    Purpose
    vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpCas9 nickase driven by EF1a promoter, guide RNA compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Insert
    nSpCas9(D10A)-MPGv6.3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    MPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202…
    Promoter
    hU6, EF1a, CBh
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 8201 - 8220 of 22800 results