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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,399 results for: BLI

Showing: 8241 - 8260 of 9399 results

  1. PX458_3XHA_den1FnCas9_KRAB_T2A_eGFP

    Plasmid
    #200910
    Purpose
    3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression
    Depositor
    Debojyoti Chakraborty
    Insert
    3XHA_den1FnCas9_KRAB_eGFP
    Use
    CRISPR
    Tags
    3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFP
    Expression
    Mammalian
    Mutation
    Changed Glutamic Acid to Arginine at 1369 position
    Promoter
    CBH
    Available Since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    200910-plasmid-map-sequence-id-407164

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  3. pGS1021

    Plasmid
    #59575
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    Twin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
    Use
    E. coli cloning vector
    Tags
    Twin-Strep tag
    Promoter
    None
    Available Since
    Aug. 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    59575-plasmid-map-sequence-id-92981

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  6. pKT-mIDH1(R132H)-Myc-FLAG-IRES-Kat

    Plasmid
    #133779
    Purpose
    Expresses IDH1 with a R132H mutation and has Myc and FLAG tags. IDH1(R132H) also has a katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    Isocitrate dehydrogenase 1 (IDH1 Human)
    Tags
    Myc and FLAG
    Expression
    Mammalian
    Mutation
    Arginine is mutated to histidine at amino acid po…
    Available Since
    Oct. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    133779-plasmid-map-sequence-id-261114

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  8. pEDF-PhdRS

    Plasmid
    #127445
    Purpose
    Double mutant of wild type mmPylRS designed for incorporation of non-cannonical amino acids in to M13 bacteriophage
    Depositor
    Wenshe Liu
    Article
    Tharp et al Nat Commun. 2020 Mar 13;11(1):1392. doi:
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Expression
    Bacterial
    Mutation
    N346A-C348A mutations on PylRS
    Promoter
    araBAD
    Available Since
    April 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    127445-plasmid-map-sequence-id-250213

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  9. pUASp YFP Ras85D WT

    Plasmid
    #52242
    Purpose
    Drosophila expression of YFP-Ras85D
    Depositor
    Matthew Scott
    Insert
    Ras85D (Ras85D Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Promoter
    GAL4
    Available Since
    March 20, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. pVRC8400-SARS-CoV-2-S2P-BA.4/5-AVI

    Plasmid
    #192112
    Purpose
    Expresses SARS-CoV-2 S2P protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Insert
    SARS-CoV-2-S2P-BA.4/5 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    T19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …
    Available Since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    192112-plasmid-map-sequence-id-374988

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  13. pCDNA3-MARS-p85(iSH2)

    Plasmid
    #205236
    Purpose
    Expresses PLEKAH5 aa 143-271 (K163A and R164A) fused to the iSH2 domain of mouse p85beta
    Depositor
    Jeremy Baskin
    Inserts
    PLEKHA5(143-271)-K163A/R164A (PLEKHA5 Human)
    p85(iSH2) (Pik3r1 Mouse)
    Tags
    Nuclear Export Sequence and mCherry
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available Since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205236-plasmid-map-sequence-id-404737

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  18. pUASp YFP Ras85D DN

    Plasmid
    #52246
    Purpose
    Drosophila expression of YFP-Ras85D dominant negative mutant
    Depositor
    Matthew Scott
    Insert
    Ras85D (Ras85D Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    S17N; DN mutation
    Promoter
    GAL4
    Available Since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 8241 - 8260 of 9399 results