We narrowed to 10,108 results for: Coli

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pQCascade-IS2

Plasmid

#140625

Purpose

Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS2. The crRNA-IS2 targets IS2 loci in Escherchia coli.

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS2

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD2 (OTU, aa 132-314)

Plasmid

#61410

Purpose

Expresses human OTUD2 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD2 (YOD1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-131 and aa 315-348.

Promoter

T7

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-N23PP/L28F/G51PEKN

Plasmid

#118585

Purpose

Bacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Expression

Bacterial

Mutation

N23 mutated to P23, L28 mutated to F28, G51 mutat…

Promoter

T7

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pHelA12

Plasmid

#176493

Purpose

Expresses the N-terminal His6-tagged SARS-CoV helicase in E coli.

Depositor

Xiaodong Cheng, Jian-Dong Huang

Article

Tanner et al J Biol Chem. 2003 Oct 10;278(41):39578-8

Insert

SARS-CoV NSP13 (ORF1ab SARS-CoV)

Tags

His6 and T7

Expression

Bacterial

Mutation

V570I

Promoter

T7

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Sumo3 PolQM1 deltains3

Plasmid

#78459

Purpose

Inducible expression in E. coli

Depositor

Sylvie Doublie, Susan Wallace

Article

Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

Insert

Polymerase theta (POLQ Human)

Tags

His and SUMO3

Expression

Bacterial

Mutation

delta 1-1791; delta 2497-2529

Promoter

T7

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only
pET29a-hNABP2-N

Plasmid

#128308

Purpose

Express hNABP2 N-terminus (1–138 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

hNABP2 N terminal fragment (OB fold) (NABP2 Human)

Tags

His

Expression

Bacterial

Mutation

N-terminal domain (1–138 aa)

Promoter

T7

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP2NhNABP1C

Plasmid

#128313

Purpose

Express chimeric protein hNABP2 N-terminus and hNABP1 C-terminus in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

chimeric protein hNABP2N+hNABP1C (NABP2, NABP1 Human)

Tags

His

Expression

Bacterial

Mutation

chimeric protein hNABP2N+hNABP1C, Y85C (please se…

Promoter

T7

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pRD404

Plasmid

#163109

Purpose

Expression of mNeonGreen_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

mNeonGreen_H-NSdbd

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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His 3C Kinase dead MKK6 1-334

Plasmid

#87882

Purpose

E. coli expression of MKK6 kinase dead

Depositor

Peter Mace

Article

Weijman et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

MKK6 (MAP2K6 Human)

Tags

3C protease site and 6xHis

Expression

Bacterial

Mutation

K82A, kinase dead mutant

Promoter

T7

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pTrc99S-ssDsbA-PD-4xDQNAT

Plasmid

#128399

Purpose

Periplasmic expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

H. influenzae protein D

Tags

4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

Expression

Bacterial

Promoter

trc

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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hSRAP.TWIN1.a13-69

Plasmid

#49815

Purpose

expression vector for SRAP(13-69) in pTWIN1 vector

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein aa 13-69 (SRA1 Human)

Tags

intein & chitin binding domain

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Available Since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pTAMAHISTEV_PfeAR480A-Q482A

Plasmid

#128946

Purpose

expresses R480A Q482A mutant of PfeA in E. coli

Depositor

James Naismith

Article

Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

Insert

ferric enterobactin receptor

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-75), …

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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p-OiRS3GG

Plasmid

#98589

Purpose

Expresses default target RNA (gI intron) and pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).

Depositor

Lydia Contreras

Article

Vazquez-Anderson et al Nucleic Acids Res. 2017 May 19;45(9):552

Inserts

group I intron
PheS cassette to be selectively replaced by unique asRNA probes

Expression

Bacterial

Promoter

pBAD and pLtetO

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-human LIC1 G domain

Plasmid

#74598

Purpose

used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 1-389

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)

Tags

N-terminal GST and Strep II

Expression

Bacterial

Promoter

T7

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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hSRAP.TWIN1.a13-87

Plasmid

#49816

Purpose

expression plasmid for SRAP(13-87) in pTWIN1 vector

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein aa 13-87 (SRA1 Human)

Tags

intein & chitin binding domain

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Available Since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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hSRAP.TWIN1.a13-215

Plasmid

#49817

Purpose

expression vector for SRAP(13-215) in pTWIN1 vector

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein aa 13-215 (SRA1 Human)

Tags

intein & chitin binding domain

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Available Since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pAJE90-Ma-tfmW-RSA2

Plasmid

#251520

Purpose

Machinery plasmid expressing Methanomethylophilus alvus aaRS/tRNA for TAG suppression and incorporation of trifluoromethyl-tryptophan (tfm-Trp) into sfGFP150TAG in E. coli

Depositor

Ryan Mehl

Article

Augustin et al J Am Chem Soc. 2026 Feb 12. doi: 10.1021

Insert

Ma-tfmW-RSA2

Expression

Bacterial

Promoter

Gln S

Available Since

March 16, 2026

Availability

Academic Institutions and Nonprofits only

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pET-28a ALDOC-K14R

Plasmid

#249431

Purpose

For E. coli expression of ALDOC-K14R

Depositor

Sheng-cai Lin

Article

Sun et al Nat Metab. 2025 Oct 22. doi: 10.1038/s42

Insert

ALDOC (ALDOC Human)

Tags

6xHis

Expression

Bacterial

Mutation

K14R

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pCW57-GFP-P2A-EcASPase

Plasmid

#252079

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of E.coli derived aspartate lyase in combination with turbo GFP using the P2A self-cleaving peptide.

Depositor

Lydia Finley

Article

Brunner et al Mol Cell. 2026 Feb 26:S1097-2765(26)0009

Insert

Codon optimized E.coli derived aspartate ammonia-lyase

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Expression

Mammalian

Mutation

Codon optimized

Promoter

TRE

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-His-PPIL1

Plasmid

#237554

Purpose

E. coli recombinant protein expression

Depositor

David Libich

Article

Parra et al Biochemistry. 2025 Aug 5;64(15):3173-317

Insert

Peptidyl-prolyl cis-trans isomerase-like 1 (PPIL1 Human)

Tags

8xHis - TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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