We narrowed to 16,448 results for: Jun

1. pCMV-eA3A-BE5-(NG)-PX458-EGFP

   Plasmid

   #229687

   Purpose

   CRISPR CBE plasmid (C to T edits): eA3A-BE5-NG engineered to include the sgRNA cloning backbone from PX458 and EGFP. Includes BbsI sites for sgRNA cloning downstream of U6 promoter.

   Depositor

   Deborah Marsh

   Article

   Dickson et al Hum Mol Genet. 2023 Jun 27:ddad105. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pInducer20 2xHA LTB4R

   Plasmid

   #226810

   Purpose

   Tet-inducible lentiviral vector for 2xHA LTB4R expression

   Depositor

   Ramiro Iglesias-Bartolome

   Article

   Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

   Insert

   LTB4R (LTB4R Human)
   

   Use

   Lentiviral

   Tags

   2xHA tag followed by a GDPPVAT linker

   Expression

   Mutation

   Sequence optimized from NP_858043.1

   Promoter

   Available since

   Jan. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR1A 2xHA GPR153

   Plasmid

   #226808

   Purpose

   Gateway entry vector for 2xHA GPR153

   Depositor

   Ramiro Iglesias-Bartolome

   Article

   Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

   Insert

   GPR153 (GPR153 Human)
   

   Use

   Gateway entry

   Tags

   2xHA tag followed by a GDPPVAT linker

   Expression

   Mutation

   Sequence optimized from NP_997253.2

   Promoter

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR1A 2xHA LTB4R

   Plasmid

   #226806

   Purpose

   Gateway entry vector for 2xHA LTB4R

   Depositor

   Ramiro Iglesias-Bartolome

   Article

   Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

   Insert

   LTB4R (LTB4R Human)
   

   Use

   Gateway entry

   Tags

   2xHA tag followed by a GDPPVAT linker

   Expression

   Mutation

   Sequence optimized from NP_858043.1

   Promoter

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pInducer20 2xHA GPR137

   Plasmid

   #226813

   Purpose

   Tet-inducible lentiviral vector for 2xHA GPR137 expression

   Depositor

   Ramiro Iglesias-Bartolome

   Article

   Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

   Insert

   GPR137 (GPR137 Human)
   

   Use

   Lentiviral

   Tags

   2xHA tag followed by a GDPPVAT linker

   Expression

   Mutation

   Sequence optimized from NP_001164351

   Promoter

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-GST-BcPC-PLC

   Plasmid

   #224255

   Purpose

   Express N-terminal HRV 3C protease cleavable GST fused to Bacillus cereus PC-PLC in E.coli

   Depositor

   Chiaki Murakami

   Article

   Murakami et al Anal Biochem. 2017 Jun 1;526:43-49. doi:

   Insert

   Bacillus cereus phospholipase C (plC, plC Bacillus cereus ATCC 14579)
   

   Use

   Tags

   GST and HRV-3C (LEVLFQGP) site

   Expression

   Bacterial

   Mutation

   The gene of Bacillus cereus ATCC 14579 was used f…

   Promoter

   lac promoter

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmiRGLO-Zc3h12a 3’UTR

   Plasmid

   #222662

   Purpose

   Luciferase reporter vector containing mouse Zc3h12a 3'UTR

   Depositor

   Silvia Monticelli

   Article

   Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

   Insert

   Zc3h12a 3'UTR (Zc3h12a Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3-FLAG-Regnase-3-HA_full-length

   Plasmid

   #222656

   Purpose

   Mammalian expression vector to express full-length Regnase-3 tagged with FLAG at N-term and HA at C-term

   Depositor

   Silvia Monticelli

   Article

   Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

   Insert

   Zc3h12c (Zc3h12c Mouse)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3-FLAG-Regnase-3_1-547 aa

   Plasmid

   #222657

   Purpose

   Mammalian expression vector to express 1-547 aa of Regnase-3 tagged with FLAG at N-term

   Depositor

   Silvia Monticelli

   Article

   Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

   Insert

   Zc3h12c 1-547 aa (Zc3h12c Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   mutated histidine 548 to a stop codon

   Promoter

   CMV

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3-FLAG-Regnase-3_1-409 aa

    Plasmid

    #222658

    Purpose

    Mammalian expression vector to express 1-409 aa of Regnase-3 tagged with FLAG at N-term

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12c 1-409 aa (Zc3h12c Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    mutated proline 410 to a stop codon

    Promoter

    CMV

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3-FLAG-Regnase-3_1-244 aa

    Plasmid

    #222659

    Purpose

    Mammalian expression vector to express 1-244 aa of Regnase-3 tagged with FLAG at N-term

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12c 1-244 aa (Zc3h12c Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    mutated asparagine 245 to a stop codon

    Promoter

    CMV

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCKC017

    Plasmid

    #226622

    Purpose

    pLenti wt hTdT template for libraries 1-2

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Insert

    Terminal deoxynucleotidyl transferase (DNTT Human)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    synonymous mutation in D2, synonymous mutation in…

    Promoter

    CMV

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pScalps-Puro-FLAG-HA-Regnase-3 D252N

    Plasmid

    #222664

    Purpose

    Lentiviral vector to express mouse Regnase-3 D252N mutant tagged with FLAG-HA at N-term

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12c (Zc3h12c Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    mutated aspartic acid 252 to asparagine to abroga…

    Promoter

    SFFV

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. p40651-EFSNS-FMR1_e1ΔCGG-24xms2

    Plasmid

    #222969

    Purpose

    MS2 mRNA reporter with FMR1 5' UTR (CGG repeats removed) and exon 1 sequence

    Depositor

    Xinyu Zhao

    Article

    Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

    Insert

    FMR1-ΔCGG 5' UTR and exon1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    31 CGG repeats deleted from 5' UTR

    Promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCKC775

    Plasmid

    #226626

    Purpose

    piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT S. pyogenes, Human)
    EF1a promoter, sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    7xTCF-LEF and EF1a

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCKC764

    Plasmid

    #226625

    Purpose

    piggyBAC pCAG-hCas9-T2A-Unbiased3

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT Chicken, S. pyogenes, Human)
    EF1a promoter, blasticidin resistance
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and EF1a

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCKC776

    Plasmid

    #226627

    Purpose

    piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT S. pyogenes, Human)
    EF1a promoter, mCherry
    

    Use

    Synthetic Biology

    Tags

    oxygen dependent degron (ODD)

    Expression

    Mammalian

    Mutation

    Promoter

    4xHRE_YB TATA and EF1a

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDH-Regnase-3 D252N-T2A-copGFP

    Plasmid

    #222661

    Purpose

    Lentiviral vector to express mouse Regnase-3 D252N

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12c (Zc3h12c Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    mutated aspartic acid 252 to asparagine to abroga…

    Promoter

    EF1α

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDH-Regnase-3 WT-T2A-copGFP

    Plasmid

    #222660

    Purpose

    Lentiviral vector to express mouse Regnase-3 WT

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12c (Zc3h12c Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    mutated stop codon prior to a T2A peptide

    Promoter

    EF1α

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3-Regnase-1 D141N

    Plasmid

    #222654

    Purpose

    Mammalian expression vector to express mouse Regnase-1 D141N mutant

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12a (Zc3h12a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutated aspartic acid 141 to asparagine to abroga…

    Promoter

    CMV

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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