We narrowed to 12,911 results for: LIC

1. pTub-alpha1-EB3-NeonGreen-T2A-iLifeact-mCherry

   Plasmid

   #175261

   Purpose

   labelling of microtubule plus end and F-actin structures

   Depositor

   Sebastián Dupraz, Frank Bradke

   Article

   Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

   Insert

   EB3-NeonGreen-T2A-iLifeact-mCherry (MAPRE3 Human)
   

   Use

   Tags

   mCherry (on iLifeact) and mNeonGreen (on EB3)

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   pTub-alpha1

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKM50

   Plasmid

   #123656

   Purpose

   Encodes Renilla luciferase under the Aedes aegypti ubiquitin UbL40 promoter for constitutive expression in mosquito cells

   Depositor

   Kevin Maringer

   Article

   Fredericks et al PLoS Negl Trop Dis. 2019 Nov 6;13(11):e0

   Insert

   Aedes aegypti UbL40 promoter
   

   Use

   Luciferase

   Tags

   Expression

   Insect

   Mutation

   Promoter

   UbL40 promoter

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONOR223 R2pH-LAMP1-3xFLAG

   Plasmid

   #157941

   Purpose

   gateway donor vector encoding ratiometric sensor of lysosomal lumenal pH

   Depositor

   Massimiliano Stagi

   Article

   Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

   Insert

   R2pH-LAMP1-3xFLAG (Lamp1 Mouse)
   

   Use

   Gateway system

   Tags

   3XFLAG, mCherry, and pHluorin

   Expression

   Mutation

   Promoter

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-FLEX-ArchT-GFP

   Plasmid

   #58851

   Purpose

   AAV-mediated expression of ArchT-GFP under the EF1a promoter, in floxed/reversed (Cre-dependent) manner

   Depositor

   Edward Boyden

   Article

   Han et al Front Syst Neurosci. 2011;5:18. Epub 201

   Insert

   ArchT-GFP
   

   Use

   AAV and Cre/Lox

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV14_NHP2L1(15.5kD)

   Plasmid

   #73065

   Purpose

   expression clone for human NHP2L1 (15.5kD) with C-terminal 3xFLAG-tag

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   NHP2L1 (15.5kD) (SNU13 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGM79

   Plasmid

   #220171

   Purpose

   CRISRPi guide RNA against MARCHF5

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   MARCHF5 (sgRNA) (MARCHF5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHIV-ARF-zsGreen

   Plasmid

   #110729

   Purpose

   Mammalian Expression of p14ARF IRES zsGreen

   Depositor

   Robert Judson-Torres

   Article

   Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

   Insert

   p14ARF IRES zsGreen (CDKN2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_hE2F7mg

   Plasmid

   #73073

   Purpose

   human E2F7 minigene (exons 11,12,13/introns cassette)

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   E2F7 (E2F7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   partial gene, exons 11,12,13 spaced by introns

   Promoter

   CMV

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-3xNLS-(G2SG2)2-Cre (JDW 1149)

   Plasmid

   #224514

   Purpose

   A Gateway compatible middle entry clone containing a 3x nuclear localization signal followed by a multiple cloning site and a flexible linker fused to Cre

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   3xNLS-(G2SG2)2-Cre
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBabe_puro_DEST_Flag_SOX2

    Plasmid

    #45270

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    MCOPS3 (SOX2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

11. SBC009876

    Plasmid

    #226283

    Purpose

    Expresses PhCFAT and ObEGS1 from trc promoter. Biosynthesis of phenylpropenes from (hydroxy)cinnamyl alcohols.

    Depositor

    Nigel Scrutton

    Article

    Robinson et al Metab Eng. 2020 Jul;60:168-182. doi: 10.

    Inserts

    Coniferyl alcohol acyltransferase
    Eugenol synthase 1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pD2sE_SC10

    Plasmid

    #219716

    Purpose

    DV2 stable E dimer with fusion loop mutation

    Depositor

    Brian Kuhlman

    Article

    Kudlacek et al Sci Adv. 2021 Oct 15;7(42):eabg4084. doi

    Insert

    DV2 sE SC10
    

    Use

    Tags

    8xHis and human serum albumin (HSA)

    Expression

    Mammalian

    Mutation

    G106D, A259W, T262R, F279W, T280P

    Promoter

    Available since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. CaMKIV-DCTK75E

    Plasmid

    #126423

    Purpose

    A kinase-dead mutant of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)

    Depositor

    Douglas Black

    Article

    Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi

    Insert

    calcium/calmodulin-dependent protein kinase IV kinase-dead mutant (CAMK4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed lysine 75 to glutamic acid

    Promoter

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-CARMA2sh-HA

    Plasmid

    #97072

    Purpose

    Expresses CARMA2sh/CARD14 isoform 3 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.3−3xFLAG−AGO2(S387A)

    Plasmid

    #220462

    Purpose

    Express S387 phospho-null mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    Human AGO2 (AGO2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Serine 387 to alanine

    Promoter

    CMV

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-N1/mCherry-DMDEx23

    Plasmid

    #211367

    Purpose

    Expresses a DMD exon 23 skipping reporter in mammalian cells (mCherry-DMDEx23)

    Depositor

    Ulrich Lächelt

    Article

    Lessl et al Bioconjug Chem. 2023 Dec 20;34(12):2263-

    Insert

    mCherry-DMDEx23
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    mCherry interrupted by mdx dystrophin exon 23 bet…

    Promoter

    CMV

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1/NDUFV1/myc-His

    Plasmid

    #127490

    Purpose

    Expresses myc-tagged NDUFV1 in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFV1 (NDUFV1 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-FRT-TO-FH-Nsp13_GC3opt

    Plasmid

    #157715

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp13_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCS2-GFP-MKLP1

    Plasmid

    #140566

    Purpose

    Expresses GFP tagged MLKP1

    Depositor

    Heidi Hehnly

    Article

    Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

    Insert

    MKLP1 (KIF23 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Polymerase expression - pCMV-ePhi29 (LM2802)

    Plasmid

    #208967

    Purpose

    Unfused ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    ePhi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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