We narrowed to 9,182 results for: Pol
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Plasmid#63128PurposeExpression of Aplysia californica AChBPDepositorInsertAChBP
ExpressionInsectPromoterPolyhedrinAvailable SinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-RPG
Plasmid#85931PurposeConstitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cellsDepositorInsertpPB-CMV-DsRed-polyA-CAG-NHEJ. Puro-T2A-EGFP
UseDual-reporter surrogateExpressionMammalianAvailable SinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD24-rnc-sfGFP
Plasmid#51560PurposeGene encoding E. coli RNaseIII cloned as a N terminal translational fussion with superfolder GFP. Expression driven by arabinose (backbone is pBAD24).DepositorInsertrnc (rnc E. coli)
TagsGLESTCRHASLAVLADERRFSA and superfolder GFPExpressionBacterialMutationContains additonal aa at the end of the sfGFP in …Available SinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
ZAK (ZAKA-c057)
Plasmid#98225PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertZAKA (MAP3K20 Human)
UseBaculovirusTagsHis-6-TEVMutationpfam00069:Pkinase 16-257PromoterpolyhedrinAvailable SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUWR-CadTS-C
Plasmid#58326PurposeDestination vector for inserting ubi-CadTS control to Drosophila melanogaster genomeDepositorInsertE-cadherin tension sensor control
ExpressionInsectPromoterpoly-ubiquitinAvailable SinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOCC292
Plasmid#118881Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mRuby3, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-mRuby3-3C-HIS6-stop-HindIII cassette
TagsmRuby3, HIS6, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC291
Plasmid#118867Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mRuby3DepositorInsertNcoI-HIS6-3C-mRuby3-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease, mRuby3ExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET23a-HsUBB
Plasmid#69562PurposeBacterial expression; in vitro transcription/translation (T7)DepositorAvailable SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
CFP-FRBx5-His
Plasmid#103784Purposeexpresses His-tagged iPOLYMER component in E. coli for protein purificationDepositorInsertCFP-FRBx5-His
ExpressionBacterialPromoterT7Available SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCSG28-SH3-588
Plasmid#185920PurposeExpresses SH3-588, a designed poly-proline peptide recruiting catechol O-methyltransferaseDepositorInsertSH3-588
Tags6x HIS tagExpressionBacterialMutationDesigned enzyme with novel interface between huma…PromoterT7Available SinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
YFP-FKBPx5
Plasmid#103775Purposeexpresses iPOLYMER component in mammalian cellsDepositorInsertYFP-FKBPx5
TagsEYFPExpressionMammalianPromoterCMVAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOCC72
Plasmid#118866Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEVDepositorInsertNcoI-HIS6-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease, mGFP, cleavable…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC61
Plasmid#118877Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
TagsmGFP, HIS6, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
YFP-FKBPx5-His
Plasmid#103785Purposeexpresses His-tagged iPOLYMER component in E. coli for protein purificationDepositorInsertYFP-FKBPx5-His
TagsEYFP, His-tagExpressionBacterialPromoterT7Available SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOCC28
Plasmid#118873Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-MBP-3C--NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease and MBP, cleavab…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET29b-eGFP-dspB(E184Q)-6xHis
Plasmid#175783PurposePlasmid for overexpression of recombinant His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
TagsHexahistidine tag and eGFPExpressionBacterialMutationE184QPromoterT7Available SinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP–COSMO–PB
Plasmid#169684PurposeCOSMO responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody variant
Tagsmodified STIM1–PB tagExpressionMammalianMutationY104WPromoterCMVAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pG23A
Plasmid#102884PurposePlasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.DepositorInsertCdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
TagsAID(71-114) and mCherryExpressionYeastPromoterNative promoterAvailable SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-LacI-p300
Plasmid#179270PurposeExpresses GFP-LacI-p300 fusion plasmidDepositorAvailable SinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-PARG shRNA
Plasmid#23260DepositorInsertpoly(ADP-ribose) glycohydrolase shRNA
UseLentiviral and RNAiExpressionMammalianAvailable SinceMarch 31, 2010AvailabilityAcademic Institutions and Nonprofits only