We narrowed to 8,430 results for: APP

1. pSPL3-ABCC8-2

   Plasmid

   #135916

   Purpose

   Encodes ABCC8 exon 2 wild type for the analysis of splicing variants

   Depositor

   Diana Valverde Pérez

   Article

   Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1

   Insert

   ATP-binding Cassette subfamily C member 8 (ABCC8 Human)
   

   Use

   Exon trapping

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCENPTΔC-dCas9-3xGFP

   Plasmid

   #198325

   Purpose

   Expresses CENPTΔC-dCas9-3xGFP protein in mammalian cells. When coupled with a guide RNA against a high repeat target locus, this can be applied to seed ectopic kinetochores at the target locus

   Depositor

   Sarah McClelland

   Article

   Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

   Insert

   CENPTΔC (CENPT Human)
   

   Use

   Lentiviral

   Tags

   EGFP x 3 and dCas9

   Expression

   Mammalian

   Mutation

   truncation - encodes only amino acids 1-375

   Promoter

   CMV-TetO

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. BPK1520-LRRK2-G2019S-ng

   Plasmid

   #180436

   Purpose

   Transiently expressing a nicking sgRNA to facilitate the introduction of LRRK2-G2019S mutation in PE3 approach

   Depositor

   Frank Soldner

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   ncRNA targeting LRRK2-G2019S (LRRK2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBPK1520-SNCA-A53T-ng

   Plasmid

   #181739

   Purpose

   Transiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A53T mutation in PE3 approach

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   Prime editing ngRNA for SNCA-A53T (SNCA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBPK1520-HEK3-CTTins-ng

   Plasmid

   #180019

   Purpose

   Transiently expressing a nicking sgRNA to facilitate the introduction of HEK3 CTT insertion in PE3 approach

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   Prime editing ngRNA for HEK3-CTTins (EPHA8 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U7

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBPK1520-SNCA-A30P-ng

   Plasmid

   #180018

   Purpose

   Transiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A30P mutation in PE3 approach

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   Prime editing ngRNA for SNCA-A30P (SNCA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-CAG-mCherry-Tandem-PSAM4-GlyR

   Plasmid

   #208361

   Purpose

   Expression of the inhibitory chemogenetic tool PSAM4-GlyR and mCherry reporter. Application of ultrapotent agonists induces neuronal silencing.

   Depositor

   David Bennett

   Article

   Perez-Sanchez et al Sci Transl Med. 2023 Oct 4;15(716):eadh3

   Insert

   mCherry-TANDEM-PSAM4-GlyR
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSPL3-ABCC8-27

   Plasmid

   #135919

   Purpose

   Encodes ABCC8 exon 27 wild type for the analysis of splicing variants

   Depositor

   Diana Valverde Pérez

   Article

   Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1

   Insert

   ATP-binding Cassette subfamily C member 8 (ABCC8 Human)
   

   Use

   Exon trapping

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FRIG-myc-Sema4D-CD4

   Plasmid

   #51606

   Purpose

   expresses myc-tagged Sema4D/CD4 non-cleavable chimera with extracellular Sema4D, intracellular CD4 in lentiviral backbone

   Depositor

   Suzanne Paradis

   Article

   Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

   Insert

   Sema4D/CD4-FLAG (Sema4d Mouse, Human)
   

   Use

   Lentiviral

   Tags

   FLAG and myc

   Expression

   Mammalian

   Mutation

   Sema4D amino acids 660-861 were replaced with CD4…

   Promoter

   RSV

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

10. ERAP1-rs27895(T)

    Plasmid

    #206143

    Purpose

    Expresses the rs27895-T allele of ERAP1, myc-tagged.

    Depositor

    Dennis Ko

    Article

    Schott et al Cell Genom. 2022 Nov 9;2(11):100207. doi

    Insert

    ERAP1 (ERAP1 Human)
    

    Use

    Tags

    myc

    Expression

    Mutation

    G346 changed to D346

    Promoter

    Available since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL3-MTS-ND1-W86-R17-G1333N-UGI-Puro

    Plasmid

    #198851

    Purpose

    Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND1) by swapping Trp codon TGA with stop codon TAA in C6 cells

    Depositor

    Bin Shen

    Article

    Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

    Insert

    ND1-W86-R17 TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGL3-MTS-ND6-W82-R15-G1333C-UGI-Puro

    Plasmid

    #198861

    Purpose

    Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND6) by swapping Trp codon TGA with stop codon TAA in C6 cells

    Depositor

    Bin Shen

    Article

    Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

    Insert

    ND6-W82-R15 TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL3-MTS-ND5-W68-R15-G1333N-UGI-Puro

    Plasmid

    #198859

    Purpose

    Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND5) by swapping Trp codon TGA with stop codon TAA in C6 cells

    Depositor

    Bin Shen

    Article

    Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

    Insert

    ND5-W68-R15 TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGL3-MTS-ND4-W24-R17-G1333N-UGI-Puro

    Plasmid

    #198857

    Purpose

    Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND4) by swapping Trp codon TGA with stop codon TAA in C6 cells

    Depositor

    Bin Shen

    Article

    Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

    Insert

    ND4-W24-R17 TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL3-MTS-ND3-Q26-R19-G1397N-UGI-Puro

    Plasmid

    #198855

    Purpose

    Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND3) by swapping Gln codon CAA with stop codon TAA in C6 cells

    Depositor

    Bin Shen

    Article

    Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

    Insert

    ND3-Q26-R19 TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL3-MTS-ND2-W30-R18-G1333N-UGI-Puro

    Plasmid

    #198853

    Purpose

    Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND2) by swapping Trp codon TGA with stop codon TAA in C6 cells

    Depositor

    Bin Shen

    Article

    Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

    Insert

    ND2-W30-R18 TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL3-MTS-CYTB-W77-R14-G1333N-UGI-Puro

    Plasmid

    #198849

    Purpose

    Employ DdCBE-mediated mtDNA editing to ablate mtProtein(CYTB) by swapping Trp codon TGA with stop codon TAA in C6 cells

    Depositor

    Bin Shen

    Article

    Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

    Insert

    CYTB-W77-R14 TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL3-MTS-COX3-W16-R15-G1333N-UGI-Puro

    Plasmid

    #198847

    Purpose

    Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX3) by swapping Trp codon TGA with stop codon TAA in C6 cells

    Depositor

    Bin Shen

    Article

    Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

    Insert

    COX3-W16-R15 TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL3-MTS-COX1-W25-R18-G1333N-UGI-Puro

    Plasmid

    #198843

    Purpose

    Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX1) by swapping Trp codon TGA with stop codon TAA in C6 cells

    Depositor

    Bin Shen

    Article

    Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

    Insert

    COX1-W25-R18 TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-MTS-ATP6-W68-R17-G1333N-UGI-Puro

    Plasmid

    #198839

    Purpose

    Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP6) by swapping Trp codon TGA with stop codon TAA in C6 cells

    Depositor

    Bin Shen

    Article

    Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

    Insert

    ATP6-W68-R17 TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge