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Mito-mEosEM

Plasmid

#132706

Purpose

application in Correlative light-electron microscopy

Depositor

Pingyong Xu

Article

Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

mitochondria targeting sequence

Tags

mEosEM

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Human IgM-Fc

Plasmid

#158214

Purpose

Express the Fc region of human IgM

Depositor

Junyu Xiao

Article

Li et al Science. 2020 Feb 28;367(6481):1014-1017

Insert

IGHM (IGHM Human)

Tags

twin strep

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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mEosEM-Lamin A

Plasmid

#132707

Purpose

application in Correlative light-electron microscopy

Depositor

Pingyong Xu

Article

Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

lamin A

Tags

mEosEM

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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FIRES-PBCS-CLV3

Plasmid

#228697

Purpose

Sensor cassette FIRE1, FIRE fluorescent ratiometric sensor

Depositor

Carolyn Sangokoya

Article

Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

Insert

FIRE1 SENSOR

Use

Synthetic Biology and Unspecified

Tags

mClover3

Expression

Mammalian

Promoter

hpgk

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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FIREC-PBCS-MC2

Plasmid

#228698

Purpose

Control cassette FIRE1, FIRE fluorescent ratiometric sensor

Depositor

Carolyn Sangokoya

Article

Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

Insert

FIRE1 CONTROL

Use

Synthetic Biology and Unspecified

Tags

mCherry

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV_miniABEmaxNG(GG)

Plasmid

#187062

Purpose

miniABEmax(GG)-SpCas9-NG

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

miniABEmax(GG)-SpCas9-NG

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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GSK3beta/pET29b

Plasmid

#16315

Depositor

Peter Klein

Article

Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

Insert

GSK3beta (gsk3b.L Frog)

Expression

Bacterial

Available Since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
Human pIgR(SC)

Plasmid

#158216

Purpose

Express the ectdomain (SC) of human pIgR

Depositor

Junyu Xiao

Article

Li et al Science. 2020 Feb 28;367(6481):1014-1017

Insert

PIGR (PIGR Human)

Tags

8×his

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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FIRES-PBCS-E670

Plasmid

#228699

Purpose

Sensor cassette FIRE2, FIRE fluorescent ratiometric sensor

Depositor

Carolyn Sangokoya

Article

Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

Insert

FIRE2 SENSOR

Use

Synthetic Biology and Unspecified

Expression

Mammalian

Promoter

hpgk

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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FIREC-PBCS-MR3

Plasmid

#228700

Purpose

Control cassette FIRE2, FIRE fluorescent ratiometric sensor

Depositor

Carolyn Sangokoya

Article

Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

Insert

FIRE2 CONTROL

Use

Synthetic Biology and Unspecified

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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NLS-mCherry

Plasmid

#221216

Purpose

control plasmid-part B of SPARK-OFF

Depositor

Xiaokun Shu

Article

Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

Insert

NLS-mCherry

Expression

Mammalian

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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PX853

Plasmid

#62885

Purpose

N-term SpCas9 piece of inducible split-5 (Cas9(N)-FRB-NES split-5).

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa 2-573)

Use

CRISPR

Tags

FRB and NES PTK2

Expression

Mammalian

Promoter

CBh

Available Since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

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pMAD7-crRNA-2

Plasmid

#170135

Purpose

For crRNA expression in monocotyledons protoplasts

Depositor

Caixia Gao

Article

Lin et al J Genet Genomics. 2021 May 3. pii: S1673

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBBR1-kan-Ptet-plu-gamma-ET_rhi145

Plasmid

#180427

Purpose

Genome engineering of Agrobacterium

Depositor

Jun Fu

Article

Bian et al Appl Environ Microbiol. 2022 Mar 8;88(5)

Insert

plu-gamma-ET_rhi145

Expression

Bacterial

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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MXW-PGK-IRES-EGFP

Plasmid

#37648

Depositor

Chang-Zheng Chen

Article

Yue et al RNA Biol. 2011 Nov-Dec;8(6):1115-23.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

mPGK

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pTORGm43GW

Plasmid

#112902

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin.

Depositor

Sebastian Schornack

Article

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-SOX2

Plasmid

#35388

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE-Sox2

Use

Lentiviral

Expression

Mammalian

Available Since

March 29, 2012

Availability

Academic Institutions and Nonprofits only
pBBR1-kan-Ptet-plu-gamma-ETh_rhi483

Plasmid

#180429

Purpose

Genome engineering of Agrobacterium

Depositor

Jun Fu

Article

Bian et al Appl Environ Microbiol. 2022 Mar 8;88(5)

Insert

plu-gamma-ETh_rhi483

Expression

Bacterial

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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Cas9m2

Plasmid

#47314

Purpose

Cas9 D10A+H840A

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

Cas9m2

Use

CRISPR

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pHR: pPGK PYL1-sfGFP-HP1A

Plasmid

#174132

Purpose

Expression of PYL1-sfGFP-HP1A

Depositor

Stanley Qi

Article

Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

Insert

PYL1-sfGFP-HP1A

Use

Lentiviral

Promoter

PGK

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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