We narrowed to 15,275 results for: grn
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Plasmid#158711PurposePlasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNADepositorInserteforCP (for crRNA cloning)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterTetOAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
plentiCRISPR-sgGOT1
Plasmid#72874PurposeThe lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).DepositorInsertsgGOT1
UseLentiviralAvailable sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRN3P_AID-Dam-only
Plasmid#136065Purposein vitro transcription for mRNADepositorInsertAID-Dam only
UseIn vitro transcriptionAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
SiC-V1-Scr
Plasmid#133042PurposeSiC-V1 vector with scramble (Scr) sgRNA. This lentivirus construct is used for stably expressing spCas9 with a dTomato reporter in a cell line of interest.DepositorInsertSpCas9
UseCRISPR and LentiviralTagsdTomatoAvailable sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-shRFP
Plasmid#125778Purposeconstitutive expression of a short-hairpin RNA targeting RFPDepositorInsertshRFP
UseCRISPRAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMZ284
Plasmid#52225PurposeExpression of sgRNA precursor targeted against ade6+DepositorInsertrrk1-sgRNA
UseCRISPRExpressionYeastPromoterrrk1Available sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PX458 V3
Plasmid#226957PurposeCBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-hGFAP-mCherry-shPtbp1
Plasmid#178589PurposeExpression of mCherry and a shRNA against Ptbp1 under the human GFAP promoterDepositorInsertmiRE-Ptbp1
UseAAVExpressionMammalianAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMOD_B2615
Plasmid#91078PurposeModule B, Promoter: AtU6, Gene: BsaI ccdb cassette for single gRNA spacer cloning, Terminator: Pol IIIDepositorInsertBsaI ccdb cassette for single gRNA spacer cloning
UseCRISPRPromoterAtU6Available sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
U6>Control(F+E)
Plasmid#60006PurposeU6 promoter driving control (F+E) sgRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertControl (F+E) sgRNA
UseCRISPRPromoterCiinte.U6Available sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJA50
Plasmid#59931PurposegRNA for cleavage at unc-58(e665) locus in C elegansDepositorInsertunc-58(e665) gRNA
UseCRISPRPromoterU6Available sinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
Multiplex-CRISPR-miR-200FAM-T2A-GFP
Plasmid#179856Purposeto delete miR200 family genes including miR-141, miR-200c, miR-429 and miR-200bDepositorInsertUseCRISPR and LentiviralExpressionMammalianAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNuc-cis
Plasmid#202796PurposeRK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killingDepositorInsertTevSpCas9, sgRNA cassette, oriT, CenArsHis
ExpressionBacterialAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAT007
Plasmid#171634PurposePlasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2DepositorInsertsspyCas9 sgRNA-TRAC
mTagBFP2
UseCRISPR and LentiviralExpressionMammalianPromoterCAG and U6Available sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRDA_186
Plasmid#133458PurposeU6 promoter expresses customizable Spyo-guide; PGK promoter expresses blasticidin resistance and 2A site provides EGFPDepositorInsertcontrol guide
UseCRISPR and LentiviralExpressionMammalianAvailable sinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Anep8_Cas9
Plasmid#117169PurposeExpressed Cas9 in Aspergillus with LIC tags for easy gRNA cloningDepositorTypeEmpty backboneUseCRISPR and Synthetic Biology; Filamentous fungi e…PromoterPkiAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSWAP_Entry_T2
Plasmid#184864PurposepSwap plasmid part containing the T2 sgRNA module for the Swap and Drop recombination system.DepositorInsertlacZalpha,ccdB
ExpressionBacterialAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSWAP_Entry_T1
Plasmid#184863PurposepSwap plasmid part containing the T1 sgRNA module for the Swap and Drop recombination system.DepositorInsertlacZalpha,ccdB
ExpressionBacterialAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Biomolecular FeedBack (FB)
Plasmid#109391PurposeThis construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.DepositorInsertpJ23100mut-dCas9-T- phtpG1-sgRNA
ExpressionBacterialAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMCRi
Plasmid#207524PurposePlasmid for CRISPRi; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpRDepositorInsertxylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR
UsePseudomonas vectorAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only