2,159 results

1. pCS2+-drAgo2

   Plasmid

   #221711

   Purpose

   Expression of zebrafish Ago2 in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   drAgo2 (ago2 Zebrafish)
   

   Use

   Fish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. hzHDAC10

   Plasmid

   #225542

   Purpose

   Expresses histone deacetylase 10 (HDAC10) humanized catalytic domain, also known as hzHDAC10, in Escherichia coli cells.

   Depositor

   David Christianson

   Article

   Herbst-Gervasoni et al ACS Chem Biol. 2020 Aug 21;15(8):2154-21

   Insert

   Histone Deacetylase 10 humanized catalytic domain (hdac10 Zebrafish)
   

   Use

   Tags

   His6-MBP tag with cleavable TEV site

   Expression

   Bacterial

   Mutation

   Mutated A24E and D94A

   Promoter

   T7

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. p5E-dr_Unc (JDW 1365)

   Plasmid

   #224482

   Purpose

   Gateway compatible 5' entry clone containing the Zebrafish unc-503 promoter with a minimal c-fos promoter and b-globin intron

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   Unc-503 promoter
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Zed_vector_tol2_+15.2prox1a:EGFP_XCA:DsRed2

   Plasmid

   #218206

   Purpose

   Zebrafish +15.2prox1a enhancer reporter in the FCLV from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vector

   Depositor

   Katarzyna Koltowska

   Article

   Panara et al Development. 2024 May 1;151(9):dev202525

   Insert

   +15.2prox1a
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2

   Plasmid

   #218207

   Purpose

   Zebrafish -2.1prox1a_1 (mouse conserved) enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vector

   Depositor

   Katarzyna Koltowska

   Article

   Panara et al Development. 2024 May 1;151(9):dev202525

   Insert

   -2.1prox1a_1
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Zed_vector_tol2_-2.1prox1a_2:EGFP_XCA:DsRed2

   Plasmid

   #218208

   Purpose

   Zebrafish -2.1prox1a_2 (not mouse conserved) enhancer reporter in the facial lymphatics at 5 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vector

   Depositor

   Katarzyna Koltowska

   Article

   Panara et al Development. 2024 May 1;151(9):dev202525

   Insert

   -2.1prox1a_2
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Tol2_-87prox1a:basEGFP_Acry:GFP

   Plasmid

   #218213

   Purpose

   Zebrafish -87prox1a enhancer reporter in the lymphatics at 5 dpf. Acry_GFP as a control in the lens

   Depositor

   Katarzyna Koltowska

   Article

   Panara et al Development. 2024 May 1;151(9):dev202525

   Insert

   -87prox1a
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDR274-gsc2-sgRNA

   Plasmid

   #184818

   Purpose

   Used to synthesize gRNA targeting exon 2 of the gsc2 gene

   Depositor

   Marnie Halpern

   Article

   Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

   Insert

   gsc2-sgRNA
   

   Use

   CRISPR; Template for sgrna synthesis

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPRISM-QF2-he1.1:YFP-rln3a-HA

   Plasmid

   #184820

   Purpose

   Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.

   Depositor

   Marnie Halpern

   Article

   Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

   Insert

   rln3a-HA
   

   Use

   CRISPR; For integration of qf2 by homology-direct…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TOPO-nmba

    Plasmid

    #184821

    Purpose

    Used to synthesize RNA probe for in situ hybridization for nmba

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    nmba (nmba Zebrafish)
    

    Use

    Template for synthesis of nmba probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TOPO-nmbb

    Plasmid

    #184822

    Purpose

    Used to synthesize RNA probe for in situ hybridization for nmbb

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    nmbb (nmbb Zebrafish)
    

    Use

    Template for synthesis of nmbb probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TOPO-nts

    Plasmid

    #184823

    Purpose

    Used to synthesize RNA probe for in situ hybridization for nts

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    nts (nts Zebrafish)
    

    Use

    Template for synthesis of nts probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GBH-2aG4-insBFP.1

    Plasmid

    #188025

    Purpose

    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

    Depositor

    Karl Clark

    Insert

    Targeted 2aGal4VP16 protein trap; secondary marker insulin BFP
    

    Use

    Targetable insertional mutagen and reporter system

    Tags

    Expression

    Mutation

    Promoter

    Endogenuous Promoter; insulin promotor

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GBH-2aG4-insGFP.1

    Plasmid

    #188028

    Purpose

    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

    Depositor

    Karl Clark

    Insert

    Targeted 2aGal4VP16 protein trap; secondary marker insulin GFP
    

    Use

    Targetable insertional mutagen and reporter system

    Tags

    Expression

    Mutation

    Promoter

    Endogenuous Promoter; insulin promotor

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GBH-2aG4-insGFP.2

    Plasmid

    #188029

    Purpose

    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

    Depositor

    Karl Clark

    Insert

    Targeted 2aGal4VP16 protein trap; secondary marker insulin GFP
    

    Use

    Targetable insertional mutagen and reporter system

    Tags

    Expression

    Mutation

    Promoter

    Endogenuous Promoter; insulin promotor

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GBH-R-insBFP.2

    Plasmid

    #188008

    Purpose

    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

    Depositor

    Karl Clark

    Insert

    Targeted mRFP protein trap; secondary marker insulin BFP
    

    Use

    Targetable insertional mutagen and reporter system

    Tags

    Expression

    Mutation

    Promoter

    Endogenuous Promoter; insulin promotor

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GBH-R-insBFP.3

    Plasmid

    #188009

    Purpose

    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

    Depositor

    Karl Clark

    Insert

    Targeted mRFP protein trap; secondary marker insulin BFP
    

    Use

    Targetable insertional mutagen and reporter system

    Tags

    Expression

    Mutation

    Promoter

    Endogenuous Promoter; insulin promotor

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GBH-R-insGFP.1

    Plasmid

    #188010

    Purpose

    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

    Depositor

    Karl Clark

    Insert

    Targeted mRFP protein trap; secondary marker insulin GFP
    

    Use

    Targetable insertional mutagen and reporter system

    Tags

    Expression

    Mutation

    Promoter

    Endogenuous Promoter; insulin promotor

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GBH-R-insGFP.2

    Plasmid

    #188011

    Purpose

    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

    Depositor

    Karl Clark

    Insert

    Targeted mRFP protein trap; secondary marker insulin GFP
    

    Use

    Targetable insertional mutagen and reporter system

    Tags

    Expression

    Mutation

    Promoter

    Endogenuous Promoter; insulin promotor

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GBH-R-insGFP.3

    Plasmid

    #188012

    Purpose

    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

    Depositor

    Karl Clark

    Insert

    Targeted mRFP protein trap; secondary marker insulin GFP
    

    Use

    Targetable insertional mutagen and reporter system

    Tags

    Expression

    Mutation

    Promoter

    Endogenuous Promoter; insulin promotor

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GBH-2aG4-gcryBFP.1

    Plasmid

    #188013

    Purpose

    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

    Depositor

    Karl Clark

    Insert

    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin BFP
    

    Use

    Targetable insertional mutagen and reporter system

    Tags

    Expression

    Mutation

    Promoter

    Endogenuous Promoter; gamma crystallin promotor

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. GBH-2aG4-gcryBFP.2

    Plasmid

    #188014

    Purpose

    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

    Depositor

    Karl Clark

    Insert

    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin BFP
    

    Use

    Targetable insertional mutagen and reporter system

    Tags

    Expression

    Mutation

    Promoter

    Endogenuous Promoter; gamma crystallin promotor

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GBH-2aG4-gcryGFP.1

    Plasmid

    #188016

    Purpose

    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

    Depositor

    Karl Clark

    Insert

    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin GFP
    

    Use

    Targetable insertional mutagen and reporter system

    Tags

    Expression

    Mutation

    Promoter

    Endogenuous Promoter; gamma crystallin promotor

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GBH-2aG4-gcryGFP.2

    Plasmid

    #188017

    Purpose

    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

    Depositor

    Karl Clark

    Insert

    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin GFP
    

    Use

    Targetable insertional mutagen and reporter system

    Tags

    Expression

    Mutation

    Promoter

    Endogenuous Promoter; gamma crystallin promotor

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GBH-2aG4-gcryGFP.3

    Plasmid

    #188018

    Purpose

    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

    Depositor

    Karl Clark

    Insert

    Targeted 2aGal4VP16 protein trap; secondary marker gamma crystallin GFP
    

    Use

    Targetable insertional mutagen and reporter system

    Tags

    Expression

    Mutation

    Promoter

    Endogenuous Promoter; gamma crystallin promotor

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GBH-2aG4-cmlc2BFP.1

    Plasmid

    #188019

    Purpose

    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

    Depositor

    Karl Clark

    Insert

    Targeted 2aGal4VP16 protein trap; secondary marker cmlc2 BFP
    

    Use

    Targetable insertional mutagen and reporter system

    Tags

    Expression

    Mutation

    Promoter

    Endogenuous Promoter; cmlc2 promotor

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GBH-2aG4-cmlc2BFP.2

    Plasmid

    #188020

    Purpose

    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

    Depositor

    Karl Clark

    Insert

    Targeted 2aGal4VP16 protein trap; secondary marker cmlc2 BFP
    

    Use

    Targetable insertional mutagen and reporter system

    Tags

    Expression

    Mutation

    Promoter

    Endogenuous Promoter; cmlc2 promotor

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GBH-2aG4-cmlc2BFP.3

    Plasmid

    #188021

    Purpose

    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

    Depositor

    Karl Clark

    Insert

    Targeted 2aGal4VP16 protein trap; secondary marker cmlc2 BFP
    

    Use

    Targetable insertional mutagen and reporter system

    Tags

    Expression

    Mutation

    Promoter

    Endogenuous Promoter; cmlc2 promotor

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. GBH-2aG4-cmlc2GFP.1

    Plasmid

    #188022

    Purpose

    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

    Depositor

    Karl Clark

    Insert

    Targeted 2aGal4VP16 protein trap; secondary marker cmlc2 GFP
    

    Use

    Targetable insertional mutagen and reporter system

    Tags

    Expression

    Mutation

    Promoter

    Endogenuous Promoter; cmlc2 promotor

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GBH-2aG4-cmlc2GFP.2

    Plasmid

    #188023

    Purpose

    Cloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafish

    Depositor

    Karl Clark

    Insert

    Targeted 2aGal4VP16 protein trap; secondary marker cmlc2 GFP
    

    Use

    Targetable insertional mutagen and reporter system

    Tags

    Expression

    Mutation

    Promoter

    Endogenuous Promoter; cmlc2 promotor

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge