Addgene

We narrowed to 2,075 results for: Pam

Showing: 1641 - 1680 of 2075 results

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pUA66 PcspA GFP Linker

Plasmid

#105604

Purpose

Expresses GFP under temperature sensitive promoter PcspA

Depositor

Pamela Silver

Article

Stirling et al Mol Cell. 2017 Nov 16;68(4):686-697.e3.

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PcspA

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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FRB-polySH35

Plasmid

#175234

Purpose

Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

NCK1 (NCK1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-DAT-myc

Plasmid

#107457

Purpose

Mammalian expression plasmid for codon optimized hDAT with internal MYC tag inserted in ECL2

Depositor

Erik Procko

Article

Young et al bioRxiv 2021.04.19.440442

Insert

Dopamine Transporter (DAT) (SLC6A3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pET23a-LIR-1 D1-D2

Plasmid

#12103

Depositor

Pamela Bjorkman

Article

Willcox et al Nat Immunol. 2003 Sep . 4(9):913-9.

Insert

Leukocyte immunoglobulin-like receptor 1 (LILRB1 Human)

Use

Tags

Expression

Bacterial

Mutation

Amino acids 1−198*

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pBluescriptKS(-)-FRB

Plasmid

#104475

Purpose

Plasmid carrying FRB with C-terminal HA tag.

Depositor

Benjamin Glick

Article

Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

Insert

FRB (MTOR Human)

Use

Phagemid

Tags

HA tag

Expression

Mutation

includes only the FRB domain

Promoter

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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FRB-polySH33

Plasmid

#175233

Purpose

Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

NCK1 (NCK1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-FKBP-HA no-pA

Plasmid

#82598

Purpose

HA epitope tag fused to C-terminus of human FK506 binding protein 12 (FKBP12) without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FKBP-HA (no pA)

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pBluescriptKS(-)-FRBx2

Plasmid

#104476

Purpose

Plasmid carrying FRBx2 with C-terminal HA tag.

Depositor

Benjamin Glick

Article

Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

Insert

FRBx2 (MTOR Human)

Use

Phagemid

Tags

HA tag

Expression

Mutation

includes only the FRB domain

Promoter

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJD427

Plasmid

#154371

Purpose

Expresses FKBP12-XB and SspBcore-FRB from constitutive promoters

Depositor

Joseph Davis

Article

Davis et al ACS Chem Biol. 2011 Nov 18;6(11):1205-13

Insert

proB-RBS(B0032)-SspBcore-FRB-terminator(B0011)-proC-RBS(B0032)-FKBP12-sspBXB-terminator(B0011)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPS1601

Plasmid

#8921

Depositor

Pamela Silver

Article

Valentini et al Genetics. 2002 Feb . 160(2):393-405.

Insert

PAB1 (PAB1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pPS832

Plasmid

#1357

Depositor

Pamela Silver

Article

Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

Insert

GSP1 (GSP1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pPS311

Plasmid

#8903

Depositor

Pamela Silver

Article

Flach et al Mol Cell Biol. 1994 Dec . 14(12):8399-40

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pPS1358

Plasmid

#8918

Depositor

Pamela Silver

Article

Kessler et al Genes Dev. 1997 Oct 1. 11(19):2545-56.

Insert

HRP1 (HRP1 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only

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PAS529 (TJF072)

Plasmid

#65603

Purpose

pETDuet-1 Containing FadD Q338R-His

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Use

Tags

6x His

Expression

Bacterial

Mutation

Q338R

Promoter

T7 lacO

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only
PAS504 (TJF067)

Plasmid

#65571

Purpose

pETDuet-1 Containing FadD F447S

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD with F447S mutation

Use

Tags

Expression

Bacterial

Mutation

F447S

Promoter

T7 lacO

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only
pPS1597

Plasmid

#8920

Depositor

Pamela Silver

Article

Valentini et al Genetics. 2002 Feb . 160(2):393-405.

Insert

TIF51A (HYP2 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pXD80ENSF7.2-Dadh-ΔElen0470(ElA2)

Plasmid

#225317

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0470) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0470)

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pPYRMandi-ABI

Plasmid

#233649

Purpose

Cellular colocalization assay (PYRMandi/ABI) for the CIP Mandi (mandipropamid)

Depositor

Richard Wombacher

Article

Poschko et al ACS Chem Biol. 2025 Jan 27. doi: 10.1021

Insert

TOMM20-mCherry-PYRMandi-P2A-eGFP-ABI

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF7.3-Dadh-ΔElen0473(ElA2)

Plasmid

#225318

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0473) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0473)

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF7.6-Dadh-ΔElen0476(ElA2)

Plasmid

#225321

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0476) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0476)

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF7.1-Dadh-ΔElen0469(ElA2)

Plasmid

#225315

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0469) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0469)

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF7.4-Dadh-ΔElen0474(ElA2)

Plasmid

#225319

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0474) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0474)

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF7.7-Dadh-ΔElen0477(ElA2)

Plasmid

#225322

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0477) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0477)

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF7.8-Dadh-ΔElen0478(ElA2)

Plasmid

#225323

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0478) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0478)

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF7.9-Dadh-ΔElen0481(ElA2)

Plasmid

#225324

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0481) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0481)

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF7.10-Dadh-ΔElen0482(ElA2)

Plasmid

#225325

Purpose

Expresses Eggerthella lenta A2 Dadh (ΔElen_0482) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh (ΔElen_0482)

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD70DA8-DadhA(cHis8)BC-DadRS-noAccs(ElA2)

Plasmid

#225326

Purpose

Expresses Eggerthella lenta A2 Dadh in Eggerthella lenta DSM 2243

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF7-DadhA(cHis8)BC-DadRS-Accs(ElA2)

Plasmid

#225302

Purpose

Expresses Eggerthella lenta A2 Dadh in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTE5417_p3WJdB_promoter dropout vector

Plasmid

#221191

Purpose

Promoter probe vector with dropout sequence for promoter sequences with 3WJdB reporter

Depositor

Tobias Erb

Article

Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Promoter Placeholder/Dropout Vector

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FKBP-GFP-emerin

Plasmid

#222415

Purpose

To express emerin with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al bioRxiv 2024.05.31.596797

Insert

Emerin (EMD Human)

Use

Tags

FKBP-GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCL1177

Plasmid

#217961

Purpose

Dual cistronic expression vector for the RAPID-release approach. H3.2-mCherry is released from mitochondria to the nucleus upon addition of rapamycin.

Depositor

Andrew Bowman

Article

Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

Insert

Histone H3 (HIST2H3PS2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FB406

Plasmid

#203627

Purpose

Promoter of the TAKA-amylase A gene from A. oryzae, maltose/starch-inducible.

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

PamyB

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PAS531 (TJF074)

Plasmid

#65605

Purpose

pETDuet-1 Containing FadD V4F, W5L -His

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Use

Tags

6x His

Expression

Bacterial

Mutation

V4F, W5L

Promoter

T7 lacO

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only
PAS509 (TJF032 L448S)

Plasmid

#65576

Purpose

pETDuet-1 Containing FadD L448S

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Use

Tags

Expression

Bacterial

Mutation

L448S

Promoter

T7 lacO

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only
PAS532 (TJF075)

Plasmid

#65606

Purpose

pETDuet-1 Containing FadD F447S-His

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Use

Tags

6x His

Expression

Bacterial

Mutation

F447S

Promoter

T7 lacO

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
PAS534 (TJF077)

Plasmid

#65608

Purpose

pETDuet-1 Containing FadD D372G-His

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Use

Tags

6x His

Expression

Bacterial

Mutation

D372G

Promoter

T7 lacO

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only
PAS535 (TJF078)

Plasmid

#65609

Purpose

pETDuet-1 Containing FadD Y9H-His

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Use

Tags

6x His

Expression

Bacterial

Mutation

Y9H

Promoter

T7 lacO

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only
PAS530 (TJF073)

Plasmid

#65604

Purpose

pETDuet-1 Containing FadD H376R-His

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Use

Tags

6x His

Expression

Bacterial

Mutation

H376R

Promoter

T7 lacO

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only
PAS503 (TJF066)

Plasmid

#65570

Purpose

pETDuet-1 Containing FadD V4F, W5L

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD with V4F, W5L mutations

Use

Tags

Expression

Bacterial

Mutation

V4F, W5L

Promoter

T7 lacO

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only
PAS516 (TJF083)

Plasmid

#65583

Purpose

pETDuet-1 Containing FadD F447R S379R

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Use

Tags

Expression

Bacterial

Mutation

F447R S379R

Promoter

T7 lacO

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

Showing: 1641 - 1680 of 2075 results

Results per page:

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