Addgene

Showing: 821 - 840 of 1289 results

Results per page:

Sort results by:

pBN074

Plasmid

#167955

Purpose

express E. coli E69 (O9a:K30) Wzc with Y705E, Y706E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y705E, Y706E

Use

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y705E, Y706E

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1514

Plasmid

#155147

Purpose

Expresses MlaFEDCB operon with MlaF C-terminus deleted [1-246], His-tag on MlaD

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-246)-mlaE-[6xHis-2xQH-TEV-mlaD]-mlaCB operon

Use

Tags

Expression

Mutation

Promoter

araBAD

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1965

Plasmid

#155152

Purpose

Expresses MlaF(1-246)-(6 aa linker)-GCN4

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-246)-GCN4_Dimerization_Domain

Use

Tags

Expression

Mutation

deleted amino acids 247-269 in MlaF; added dimeri…

Promoter

araBAD

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1966

Plasmid

#155153

Purpose

Expresses MlaF(1-246)-(10 aa linker)-GCN4

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-246)-GCN4_Dimerization_Domain

Use

Tags

Expression

Mutation

deleted amino acids 247-269 in MlaF; added dimeri…

Promoter

araBAD

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1967

Plasmid

#155154

Purpose

Expresses MlaF(1-246)-(14 aa linker)-GCN4

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

mlaF(1-246)-GCN4_Dimerization_Domain

Use

Tags

Expression

Mutation

deleted amino acids 247-269 in MlaF; added dimeri…

Promoter

araBAD

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE30-VRN1-R249E-R289E-R296E

Plasmid

#159532

Purpose

Expresses Arabidopsis thaliana VERNALIZATION1 mutant, with R249E, R289E and R296E mutations, in E. coli

Depositor

Joshua Mylne

Article

King et al J Biol Chem. 2013 Feb 1;288(5):3198-207.

Insert

VERNALIZATION1 (VRN1 Mustard Weed)

Use

Tags

Six-Histidine tag

Expression

Bacterial

Mutation

Three charge reversal mutations, namely R249E R28…

Promoter

T5

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX2T AtFC1

Plasmid

#136363

Purpose

Bacterial expression of recombinant Arabidopsis thaliana ferrochelatase1

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

Full-length cDNA of Arabidopsis thaliana ferrochelatase1 (FC1 Mustard Weed)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Lac

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCASP-SptP120-K55-HilA

Plasmid

#153329

Purpose

Triggers secretion of SptP120 fused to the K55 DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-K55 DARPin

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCASP-SptP120-E3_5-HilA

Plasmid

#153326

Purpose

Triggers secretion of SptP120 fused to the E3_5 DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-E3_5 DARPin

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCASP-SptP120-K27-HilA

Plasmid

#153328

Purpose

Triggers secretion of SptP120 fused to the K27 DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-K27 DARPin

Use

Salmonella expression

Tags

Expression

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET48 AtGUN1-PS

Plasmid

#136358

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 918)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 918 (GUN1 Mustard Weed)

Use

Tags

TRX-His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET48 AtGUN1-SMR1

Plasmid

#136361

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 709 to 918)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 709 to 918 (GUN1 Mustard Weed)

Use

Tags

TRX-His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET48 AtGUN1-SMR2

Plasmid

#136362

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 781 to 918)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 781 to 918 (GUN1 Mustard Weed)

Use

Tags

TRX-His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET48 AtGUN1-PPR1

Plasmid

#136359

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 708)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 708 (GUN1 Mustard Weed)

Use

Tags

TRX-His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET48 AtGUN1-PPR2

Plasmid

#136360

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 619)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 619 (GUN1 Mustard Weed)

Use

Tags

TRX-His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CIB-VP64 (B1016)

Plasmid

#92037

Purpose

Expresses CIB1 (Full-length, with endogenous NLS) fused to VP64 activation domain (4xVP16 inserts)

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CIB1-VP64 (CIB1 Mustard Weed)

Use

Tags

Fusion of plant CIB1 with VP64 activation domain.…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
CRY-GalVP16l (B694)

Plasmid

#92030

Purpose

expresses CRY2 (Full length, mutant NLS) fused to Gal4DNA (residues 1-147) fused to VP16 activation domain (long form)

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CRY2 (CRY2 Mustard Weed)

Use

Tags

GalBD-VP16

Expression

Mammalian

Mutation

NLS sequence of CRY2 mutated

Promoter

CMV

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBN095

Plasmid

#173765

Purpose

express E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation

Use

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

delete motif 3

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBN215

Plasmid

#167962

Purpose

express E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E

Use

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y715E, Y717E, Y718E

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBN261

Plasmid

#167966

Purpose

express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E

Use

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y713E, Y715E, Y717E

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge