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HJP-224-actin5C^Syn21Gal80^IVS-Syn21myr-tdTomato-10UAS-mCD8GFP-p10w

Plasmid

#162481

Purpose

A bicistronic construct - FloxGal80 and UAS-mCD8GFP

Depositor

Benjamin White

Article

Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

Insert

Cre80Tom-GFP

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7b-T2A-tdTomato-p10

Plasmid

#172917

Purpose

LexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Insert

jGCaMP7b-T2A-tdTomato

Use

Tags

6xHis and T2A-tdTomato

Expression

Insect

Mutation

Promoter

hsp70

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7s-T2A-tdTomato-p10

Plasmid

#172920

Purpose

LexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Insert

jGCaMP7s-T2A-tdTomato

Use

Tags

6xHis and T2A-tdTomato

Expression

Insect

Mutation

Promoter

hsp70

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7f-T2A-tdTomato-p10

Plasmid

#172919

Purpose

LexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Insert

jGCaMP7f-T2A-tdTomato

Use

Tags

6xHis and T2A-tdTomato

Expression

Insect

Mutation

Promoter

hsp70

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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AiP11980 - pAAV-DLX2.0-minRho-dTomato-10aa-H2B-WPRE3-BGHpA (Alias: CN1980)

Plasmid

#229934

Purpose

DLX2.0 is an enhancer sequence, designed to drive nuclear dTomato expression in forebrain GABAergic neurons

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

dTomato-10aa-H2B

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pB6-Hipp11-H2B-sfGFP-T2A-tdTomato-cre reporter-3pA stop-FNF-DTA

Plasmid

#183027

Purpose

B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-H2B-sfGFP-T2A-tdTomato-WPRE-bpA allele targeting vector, low copy number

Depositor

Mario Capecchi

Insert

H2B-sfGFP-T2A-tdTomato

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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CAG-loxP-3xpA-loxP-H2B-sfGFP+pCI syntron-T2A-tdTomato+RbG and HsbG syntrons

Plasmid

#172480

Purpose

H2B-sfGFP with a synthetic intron from the pCI expression vector, T2A, and tdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin gene

Depositor

Mario Capecchi

Insert

H2B-sfGFP-T2A-tdTomato

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
KHBD00516

Plasmid

#39617

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG7508 (ato Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only
His-myc-Med20 pBacPAK8

Plasmid

#15370

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

Insert

Med20

Use

Baculovirus construction

Tags

His and myc

Expression

Mutation

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pET32b-FLJ23445

Plasmid

#15434

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

FLJ23445

Use

Tags

His and Trx

Expression

Bacterial

Mutation

N/A

Promoter

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-GAS41

Plasmid

#15428

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

GAS41 (Yeats4 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Promoter

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-MED17

Plasmid

#15410

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

Insert

MED17 (MED17 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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FLAG-Med29 pBacPAK8

Plasmid

#15369

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

Insert

Med29 (MED29 Human)

Use

Baculovirus construction

Tags

FLAG

Expression

Mutation

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-MED29

Plasmid

#15409

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

Insert

MED29 (MED29 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-MED8

Plasmid

#15406

Depositor

Joan Conaway, Ronald Conaway

Article

Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

Insert

MED8 (MED8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-MED6

Plasmid

#15411

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

Insert

MED6 (Med6 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pET19b-MED17

Plasmid

#15412

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

Insert

MED17 (MED17 Human)

Use

Tags

His

Expression

Bacterial

Mutation

N/A

Promoter

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pGEX4T-2 MED8

Plasmid

#15436

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED8 (MED8 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

N/A

Promoter

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-FLJ23445

Plasmid

#15429

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

FLJ23445 (Thoc7 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pET19b-MED29

Plasmid

#15415

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

Insert

MED29 (MED29 Human)

Use

Tags

His

Expression

Bacterial

Mutation

N/A

Promoter

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only