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pCIneo-V5-SBP-MBP-HsNot4-del190-274_AG

Plasmid

#148782

Purpose

Mammalian Expression of HsNot4-del190-274

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4-del190-274 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-NvG-HsNot8_13-240_U

Plasmid

#147649

Purpose

Bacterial Expression of HsNot8_13-240

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsNot8_13-240 (CNOT8 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1674-1962-dsRNAres_AC

Plasmid

#148402

Purpose

Insect Expression of DmNot1_1674-1962-dsRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmNot1_1674-1962-dsRNAres (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

2 silent mutations compared to the sequence given…

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1711-2505-dsRNAres_R

Plasmid

#147433

Purpose

Insect Expression of DmNot1_1711-2505-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1711-2505-dsRNAres (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

4 silent mutations compared to the sequence given…

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1-1148_I

Plasmid

#146604

Purpose

Insect Expression of DmNot1_1-1148

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1-1148 (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

4 silent and two non silent N605S, K759M mutation…

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot7-M141R_R

Plasmid

#147379

Purpose

Mammalian Expression of HsNot7-M141R

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot7-M141R (CNOT7 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-NvG-HsNot7_9-260_R

Plasmid

#147369

Purpose

Bacterial Expression of HsNot7_9-260

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot7_9-260 (CNOT7 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4_1-274_AG

Plasmid

#148750

Purpose

Mammalian Expression of HsNot4_1-274

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_1-274 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMCh-607

Plasmid

#92258

Purpose

Plasmid for expression of NHA-tagged human TNRC6C CED 7W7A with seven mutated W-motifs in mammalian cells

Depositor

Witold Filipowicz, Marina Chekulaeva

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

TNRC6C CED (TNRC6C Human)

Use

Tags

NHA

Expression

Mammalian

Mutation

C-terminal effector domain (CED): aa 1369-1690 wi…

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMCh-633

Plasmid

#92253

Purpose

Plasmid for expression of GST-tagged human TNRC6C 7W7A CED with seven mutated W-motifs in mammalian cells

Depositor

Witold Filipowicz, Marina Chekulaeva

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

TNRC6C CED (TNRC6C Human)

Use

Tags

GST

Expression

Mammalian

Mutation

C-terminal effector domain (CED): aa 1369-1690 wi…

Promoter

EF1A

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1-V1251R_R

Plasmid

#147376

Purpose

Mammalian Expression of HsNot1iso1-V1251R

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1-V1251R (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

A deletion of 5 AA 822-827 SKMKPS-> T and one …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-HsHelz_1475-1642del1595-1609-GB1His6_AH

Plasmid

#148889

Purpose

Bacterial Expression of HsHelz_1475-1642-del1595-1609

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1475-1642-del1595-1609 (HELZ Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-HsHelz_1643-1792del1746-1764-GB1His6_AH

Plasmid

#148892

Purpose

Bacterial Expression of HsHelz_1643-1792-del1746-1764

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1643-1792-del1746-1764 (HELZ Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-HsHelz_1793-1942del1890-1907-GB1His6_AH

Plasmid

#148895

Purpose

Bacterial Expression of HsHelz_1793-1942-del1890-1907

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1793-1942-del1890-1907 (HELZ Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9-(BB)-2A-Puro-HsHelz-sgRNA_AH

Plasmid

#148897

Purpose

Mammalian Expression of HsHelz-sgRNA

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz-sgRNA (HELZ Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLIB-2STREP-HsHelz_1051-1942-GSSG-MBP_AG

Plasmid

#148795

Purpose

Mammalian Expression of HsHelz_1051-1942-GSSG

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1051-1942-GSSG (HELZ Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-V5-SBP-MBP-HsNot9shRNA2res_AG

Plasmid

#148828

Purpose

Mammalian Expression of HsNot9-shRNA2res

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot9-shRNA2res (CNOT9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-V5-SBP-MBP-HsNot9-V181E-shRNA2res_AG

Plasmid

#148829

Purpose

Mammalian Expression of HsNot9-V181E-shRNA2res

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot9-V181E-shRNA2res (CNOT9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-HsNot10_25-707-HsNot11_257-498-CvH_AF

Plasmid

#148731

Purpose

Bacterial Expression of HsNot10_25-707-HsNot11_257-498

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot10_25-707-HsNot11_257-498 (CNOT10 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-HsNot9_19-285_AF

Plasmid

#148733

Purpose

Bacterial Expression of HsNot9_19-285

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot9_19-285 (CNOT9 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge